Polyhydroxyalkanoate production from whey by Pseudomonas hydrogenovora

Koller, Martin; Bona, Rodolfo; Chiellini, Emo; Fernandes, Elizabeth Grillo; Horvat, Predrag; Kutschera, Christoph; Hesse, Paula Johanna; Braunegg, Gerhart

doi:10.1016/j.biortech.2007.09.049

Cited by 196 publications

(104 citation statements)

References 20 publications

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“…H. mediterranei on hydrolyzed whey as substrate produced P(3HB:6 % 3HV), which improved to P(3HB:21.8 % 3HV:5.1 % 4HB) while the yield increased to 87.5 % when sodium valerate and c-butyrolactone was used to supplement the feed [38]. Pseudomonas can produce comparatively wider range of PHA on different wastes such as P(3HB:3HV) on whey [53], P(3HO:3HD:3HDD) on coprah oil [54], P(3HHx:3HO:3HD:3HDD:3HTD) on delignified rye grass hydrolysate [55] and P(3HHx:3HO:3HD:3HDD: 3HDDE:3HTD:3HTDE) on soy molasses [26]. As also observed with FAs, oily waste can provide a wider range of monomers in PHA [56,57].…”

Section: Substrate and Feeding Regimementioning

confidence: 99%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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Polyhydroxyalkanoates (PHAs) as an alternative to synthetic plastics have been gaining increasing attention. Being natural in their origin, PHAs are completely biodegradable and eco-friendly. However, consistent efforts to exploit this biopolymer over the last few decades have not been able to pull PHAs out of their nascent stage, inspite of being the favorite of the commercial world. The major limitations are: (1) the high production cost, which is due to the high cost of the feed and (2) poor thermal and mechanical properties of polyhydroxybutyrate (PHB), the most commonly produced PHAs. PHAs have the physicochemical properties which are quite comparable to petroleum based plastics, but PHB being homopolymers are quite brittle, less elastic and have thermal properties which are not suitable for processing them into sturdy products. These properties, including melting point (T m ), glass transition temperature (T g ), elastic modulus, tensile strength, elongation etc. can be improved by varying the monomeric composition and molecular weight. These enhanced characteristics can be achieved by modifications in the types of substrates, feeding strategies, culture conditions and/or genetic manipulations.

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Section: Substrate and Feeding Regimementioning

confidence: 99%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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“…In shaking flask experiments, the utilization of whey permeate as a cheap substrate was compared to the utilization of pure glucose and galactose for bacterial growth under balanced conditions as well as for the production of PHB under nitrogen limitation. The strain P. hydrogenovora DSM 1749 is able to cometabolize glucose and galactose from lactose-hydrolyzed whey permeate to produce PHB (Koller et al, 2008) This indicates that for an organism to efficiently use whey as substrate, it must be able to metabolize the lactose as its carbon source. Such organisms should produce sufficient lactose hydrolyzing enzymes (β-galactosidase) to hydrolyze the lactose to its monomers (Khanafari and Sepahei, 2007).…”

Section: Effect Of Different Carbon Sources On Phb Production By B Cmentioning

confidence: 99%

Production of Poly-β-hydroxybutyric acid (PHB) by Bacillus cereus

Belal¹,

Farid²

2016

Int.J.Curr.Microbiol.App.Sci

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“…19 A verificação de quais fontes de carbono são utilizadas por cada microrganismo serve para direcionar a escolha do substrato para a produção de PHAs em grandes volumes, isto é, cultura submersa em biorreatores. 20 Todos os microrganismos obtidos no presente trabalho utilizam a glicose como fonte de carbono e a maioria utiliza também sacarose e manitol (Tabela 2). Para verificar a utilização de outras fontes de carbono como óleo e soro de leite, os microrganismos foram cultivados em meio mínimo e a turbidez averiguada após 72 h. A turbidez do meio de cultura geralmente está relacionada com a concentração de células do microrganismo, mas no caso de cultivo em meio para síntese de PHAs, esta é relacionada com a quantidade intracelular do polímero, isto é, quanto mais turvo o meio, devido a opacidade da cultura presente, provavelmente houve mais síntese do bioplástico.…”

Section: Fontes De Carbono E Turbidez Em Meio Mínimounclassified

Poliphasic Screening of Polyhydroxyalkanoate-Producing Microorganisms

Silva¹,

Santos²,

Santos³

et al. 2016

Química Nova

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publicado na web em 10/05/2016 POLIPHASIC SCREENING OF POLYHYDROXYALKANOATE-PRODUCING MICROORGANISMS. Polyhydroxyalkanoates (PHAs) are fully biodegradable biopolyesters produced by many prokaryotes and accumulated as insoluble cytoplasmatic inclusions. The detection of these intracellular granule is usually provided using lipofilic azodyes, which are not specific. Another way to screen PHA-producing bacteria is through culture-independent molecular techniques such Polymerase Chain Reaction (PCR), and the search for new PHA-producer strains is essential to reduce the cost at industrial level. The application of these both methods is desirable. In the present study, 24 bacteria isolated from soil of the Atlantic forest in Maceió (AL, Brazil) and from agri-industrial sludge (Coruripe-AL, Brazil) were studied regarding to their capacity of growing in mineral salt medium, as indicative of PHA synthesis. All strains were submitted to biochemical characterization, whilst PCR has proved that isolates BMA-05, BMA-10, BMA-13 and BDL-07 has the gene phaC, which encodes a PHA synthase, the key for PHA synthesis. The amplification and sequencing of their 16S r-DNA region was able to identify these bacteria respectively as Pseudomonas fluorescens, Enterobacter aerogenes, Klebsiella oxytoca and Bacillus pumilus. The same minimal medium supplement with peptone demonstrated to induce growing of those strains.Keywords: polyhydroxyalkanoate; molecular identification; PhaC synthase. INTRODUÇÃOPara ser considerado um plástico degradável, a molécula deve apresentar cadeia com ou sem ramificação ou com grupos funcionais éster, amida ou acetal.1 Comumente, tais características não são atendidas pelos polímeros sintéticos, e sua degradação no ambiente dá-se muito lentamente. Por outro lado, os plásticos denominados biodegradáveis possuem os requisitos apresentados para tal e por isso são rapidamente decompostos por muitas espécies bacterianas e microalgas. Conforme descrito por Khanna e Srivastava, 2 distribuem-se em três categorias: a) a dos polímeros sintéticos suscetíveis a degradação e/ou ataque enzimático de microrganismos, mas que não atendem a todas as características necessárias para exploração comercial; b) a dos bioplásticos baseados no polissacarídeo amido, que são parcialmente degradáveis, pois os microrganismos degradam o amido mas não o polímero sintético que faz parte da composição desses produtos; c) a dos polihidroxialcanoatos (PHAs) produzidos por diversos microrganismos e armazenados intracelularmente como inclusões amorfas, mas que são totalmente degradáveis por organismos vivos.Nesse sentido, desde a década de 90 do século passado, o Brasil vem dedicando um considerável esforço para o desenvolvimento de processos visando a geração em larga escala e utilização de polihidroxialcanoatos (PHAs) em produtos.3 Cerca de 150 monômeros diferentes já foram identificados como constituintes de PHAs produzidos por bactérias a partir de diversas fontes de carbono. 4 Vale ressaltar que, na maioria das vezes, esse polímero biodegra...

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Polyhydroxyalkanoate production from whey by Pseudomonas hydrogenovora

Cited by 196 publications

References 20 publications

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Production of Poly-β-hydroxybutyric acid (PHB) by Bacillus cereus

Poliphasic Screening of Polyhydroxyalkanoate-Producing Microorganisms

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