Biopolymers are gaining much interest and are considered appropriate substitutes for petroleum-based plastics. It is used for various applications in the medical, agriculture, and packaging fields. The bacterial strains from river soil samples were isolated and screened for biopolymer production. The objective of the present study was to produce polyhydroxybutyrate (PHB) from the isolated strains under optimized conditions in a shake flask. The optimization was done with the software tool Design expert -Response surface methodology (RSM). The optimum environmental conditions were: initial pH 7.0, Carbon Source 35g/l, and Nitrogen source 1g/l. Under these controlled conditions, biopolymer yield was obtained 2.25g/L. The FT-IR spectra of extracted biopolymer indicate symmetric (O-C-O) presence at 1600cm -1 and asymmetric absorption peak at 1400 cm -1 . The TGA result indicates the thermal degradation of the obtained biopolymer was at 106 ℃. The obtained results suggest that these biopolymers may be similar to PHB.
INTRODUCTION:Biopolymer is a biodegradable polymeric material obtained from various biological sources such as plants, microorganisms, etc. It is the fastest and most efficient developing method to provide alternatives to fossil-fuel-based polymers and the best way to reduce global warming and pollution caused by conventional plastics 1 . Various kinds of biopolymers are available based on their source, degradation type, structural and functional backbone.