Polyhydroxyalkanoates: a review of microbial production and technology application

Alves, Aline de Andrade; Siqueira, Edmilson Clarindo de; Barros, M. P. S.; Silva, Páblo Eugênio da Costa e; Houllou, Laureen Michelle

doi:10.1007/s13762-022-04213-9

Cited by 32 publications

(37 citation statements)

References 91 publications

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“…Since biopolymers such as polysaccharides are commonly produced as storage materials in living cells ( Gonzalez-Gutierrez et al, 2010 ), the biopolymers can be easily depolymerized by certain species of microorganisms such as fungi, yeasts, and bacteria ( Sirohi et al, 2020 ). Hitherto, several kinds of biodegradable plastics produced by microbial cells, including polyhydroxyalkanoates (PHA) ( Alves et al, 2022 ), poly(butylene adipate-co-terephthalate) ( Jian et al, 2020 ), and polylactic acid (PLA) ( Taib et al, 2022 ), have attracted extensive attention worldwide. Among all types of biopolymers, poly(3-hydroxybutyrate) (PHB) has attracted tremendous research attention in recent years due to its high biodegradability ( Getachew and Woldesenbet, 2016 ) as well as significantly lower energy requirement and CO 2 emission during the production process ( Lopez-Arenas et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

A Review on Enhancing Cupriavidus necator Fermentation for Poly(3-hydroxybutyrate) (PHB) Production From Low-Cost Carbon Sources

Zhang

Jiang

Tsui

et al. 2022

Front. Bioeng. Biotechnol.

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In the context of a circular economy, bioplastic production using biodegradable materials such as poly(3-hydroxybutyrate) (PHB) has been proposed as a promising solution to fundamentally solve the disposal issue of plastic waste. PHB production techniques through fermentation of PHB-accumulating microbes such as Cupriavidus necator have been revolutionized over the past several years with the development of new strategies such as metabolic engineering. This review comprehensively summarizes the latest PHB production technologies via Cupriavidus necator fermentation. The mechanism of the biosynthesis pathway for PHB production was first assessed. PHB production efficiencies of common carbon sources, including food waste, lignocellulosic materials, glycerol, and carbon dioxide, were then summarized and critically analyzed. The key findings in enhancing strategies for PHB production in recent years, including pre-treatment methods, nutrient limitations, feeding optimization strategies, and metabolism engineering strategies, were summarized. Furthermore, technical challenges and future prospects of strategies for enhanced production efficiencies of PHB were also highlighted. Based on the overview of the current enhancing technologies, more pilot-scale and larger-scale tests are essential for future implementation of enhancing strategies in full-scale biogas plants. Critical analyses of various enhancing strategies would facilitate the establishment of more sustainable microbial fermentation systems for better waste management and greater efficiency of PHB production.

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Section: Introductionmentioning

confidence: 99%

A Review on Enhancing Cupriavidus necator Fermentation for Poly(3-hydroxybutyrate) (PHB) Production From Low-Cost Carbon Sources

Zhang

Jiang

Tsui

et al. 2022

Front. Bioeng. Biotechnol.

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“…Synthetic plastics have become an integral part of contemporary society and are present in almost all utensils and products used in everyday life. They were designed with high-performance and quality materials and, therefore, have a very long service life (Alves et al, 2022). However, the non-biodegradable nature of these plastics favors their accumulation in difficult-to-handle amounts, generating serious environmental problems (Tohme et al, 2018;Castro et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…The waste generated by these plastics represents about 8% by weight and 25% by volume of total municipal solid waste (Samui & Kanai, 2019). In this sense, there is a permanent need to replace conventional plastics with biodegradable plastics Tohme et al, 2018;Alves et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, bio-based polymers have been considered one of the main alternatives to petrochemical plastics (Samui and Kanai, 2019;Alves et al, 2022). Among the biopolymers, polyhydroxyalkanoates (PHAs) have been gaining importance among researchers due to their non-toxic, biodegradable, and biocompatible nature (Alves et al, 2022). They are classified into three different groups according to their monomeric units; short, medium, and long-chain-length PHAs.…”

Section: Introductionmentioning

confidence: 99%

“…The PHAs have recognized applications in the food, pharmaceutical, and biomedical sectors. Although PHAs are considered an alternative to petroleum-based polymers, the high cost of production limits their availability on a commercial scale (Amaro et al, 2019;Alves et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Co-production of polyhydroxyalkanoates and levan by Halomonas smyrnensis AAD6T

Siqueira

Houllou²

2022

RSD

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The simultaneous production of microbial polymers levan and poly[3-hydroxybutyrate] (PHB), a type of polyhydroxyalkanoates, was investigated in this work. The study involved the fermentation of sucrose and molasses by H. smyrnensis AAD6T (BAE2 strain) to produce PHB (intracellular) and levan (extracellular). Both polymers were isolated and characterized by FTIR. Levan was also characterized by thin-layer chromatography (TLC) and viscosimetric analysis. The amount of biomass was 25 g until the end of fermentation. The PHB rate was 0.015 g in both media and the average PHB productivity was 6.0 x 10-4 g PHB/g biomass. The highest rate of levan was 9 g/L in the range of 72–80 h, in the molasses-based medium. The FTIR spectra showed specific signals for each of the polymers, such as the peak at 1700 for the carbonyl group of esters for the PHB and signals at 900 and 800, which are typical signals for levan fructose rings. Furthermore, acid hydrolysis of levan revealed that it was formed only by fructose, as confirmed by TLC With this study, H. smyrnensis AAD6T BAE2 co-produced PHB and levan using a low-cost carbon source, showing great potential in reducing biopolymer manufacturing costs.

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Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers

de Siqueira,

de Andrade Alves,

da Costa e Silva

et al. 2023

Biotechnology Progress

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Polyhydroxyalkanoates (PHAs) and exopolysaccharides (EPSs) belong to a class of abundant biopolymers produced by various fermenting microorganisms. These biocompounds have high value‐added potential and can be produced concurrently. Co‐production of PHAs and EPSs is a strategy employed by researchers to reduce costs associated with large‐scale production. EPSs and PHAs are non‐toxic, biocompatible, and biodegradable, making them suitable for various industrial sectors, including packaging and the medical and pharmaceutical industries. These biopolymers can be derived from agro‐industrial residues, thus contributing to the bioeconomy by producing high‐value‐added products. This review investigates approaches for simultaneously synthesizing PHAs and EPSs using different carbon sources and microorganisms.

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Polyhydroxyalkanoates: a review of microbial production and technology application

Cited by 32 publications

References 91 publications

A Review on Enhancing Cupriavidus necator Fermentation for Poly(3-hydroxybutyrate) (PHB) Production From Low-Cost Carbon Sources

A Review on Enhancing Cupriavidus necator Fermentation for Poly(3-hydroxybutyrate) (PHB) Production From Low-Cost Carbon Sources

Co-production of polyhydroxyalkanoates and levan by Halomonas smyrnensis AAD6T

Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers

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