Polyhydroxyalkanoates production from fermented domestic wastewater using phototrophic mixed cultures

Almeida, Juliana Roda; Serrano, Esteban; Fernández, Maikel; Fradinho, Joana; Oehmen, Adrian; Reis, Maria A. M.

doi:10.1016/j.watres.2021.117101

Cited by 26 publications

(23 citation statements)

References 28 publications

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“…This study analyses the bacterial communities of reported phototrophic mixed culture (PMC) systems that were enriched in phototrophic purple bacteria (PPB) for the production of PHA. For simplification, these systems were divided into two sets: one set regarding the operation of PMCs fed with synthetic medium, where acetate was the sole carbon source (Table 1, cultures S1 to S5) [15][16][17]23] and a second set, regarding the operation of PMCs that were fed with a real waste feedstock composed of a fermented mixture of domestic wastewater supplemented with 1% v/v molasses (Table 1, cultures R1 to R6) [24]. A local pond sediment was the original inoculum of cultures S1 to S5, and R0 was the original inoculum used to seed cultures R1 to R6 and was collected from a high-rate algal pond treating domestic wastewater at El Torno WWTP (Aqualia) at Chiclana, Andalucia, Spain.…”

Section: Phototrophic Mixed Cultures Operationmentioning

confidence: 99%

“…Cultures R1 to R6 were operated under 12 h light/12 h dark conditions and all were fed with a real waste feedstock consisting of a fermented mixture of domestic wastewater supplemented with 1% molasses. The feed was mostly composed of VFA (acetic, propionic, butyric and valeric acid) except for the R2 and R5 feedstock, which contained leftover sugars due to incomplete upstream feedstock fermentation [24].…”

Section: Fermented Real Waste Feeding (Cultures R1 To R6)mentioning

confidence: 99%

“…Cultures R1 to R3 were subjected to a permanent feast regime, and despite the reactors being open, the constant carbon presence led to a very low microalgae content (thus minimal oxygen production) and resulted in anaerobic conditions [24]. Under this environment, community changes occurred due to variations in the OLR and fluctuations of the feedstock composition.…”

Section: Permanent Feast Operation (R1 To R3)mentioning

confidence: 99%

“…The decrease in the OLR is another factor that impacted the microbial culture composition in the systems fed with fermented wastewater. R3 was operated under low OLR with the permanent presence of carbon, but at low concentrations (<2 Cmmol/L) [24]. Considering the sequencing results, the culture became highly enriched in Rhodopseudomonas (>84% abundance), with a moderate presence of Rhizobium (11.2%), a minor presence of Rhodobacter (1.3%) and no Chromatiaceae detection (Table 3).…”

Section: Permanent Feast Operation (R1 To R3)mentioning

confidence: 99%

“…Feast and Famine Operation (R4 to R6) Cultures R4 to R6 were operated under a feast and famine regime in an open system that, unlike cultures S1 to S5, was not sparged with an inert gas. During the feast phase, low oxidation reduction potential (ORP) levels were achieved in R4 to R6, while during the famine phase, the ORP would increase due to surface oxygen transfer and oxygen production by microalgae [24]. In cultures R4 to R6, the OLR gradually increased (Table 1), which extended the feast phase length, leading to fewer hours of oxygen availability during the famine phase.…”

Section: Permanent Feast Operation (R1 To R3)mentioning

confidence: 99%

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Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures

et al. 2022

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Phototrophic mixed cultures (PMC) are versatile systems which can be applied for waste streams, valorisation and production of added-value compounds, such as polyhydroxyalkanoates (PHA). This work evaluates the influence of different operational conditions on the bacterial communities reported in PMC systems with PHA production capabilities. Eleven PMCs, fed either with acetate or fermented wastewater, and selected under either feast and famine (FF) or permanent feast (PF) regimes, were evaluated. Overall, results identified Chromatiaceae members as the main phototrophic PHA producers, along with Rhodopseudomonas, Rhodobacter and Rhizobium. The findings show that Chromatiaceae were favoured under operating conditions with high carbon concentrations, and particularly under the PF regime. In FF systems fed with fermented wastewater, the results indicate that increasing the organic loading rate enriches for Rhodopseudomonas, Rhizobium and Hyphomicrobiaceae, which together with Rhodobacter and Chromatiaceae, were likely responsible for PHA storage. In addition, high-sugar feedstock impairs PHA production under PF conditions (fermentative bacteria dominance), which does not occur under FF. This characterization of the communities responsible for PHA accumulation helps to define improved operational strategies for PHA production with PMC.

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Section: Phototrophic Mixed Cultures Operationmentioning

confidence: 99%

Section: Fermented Real Waste Feeding (Cultures R1 To R6)mentioning

confidence: 99%

Section: Permanent Feast Operation (R1 To R3)mentioning

confidence: 99%

Section: Permanent Feast Operation (R1 To R3)mentioning

confidence: 99%

Section: Permanent Feast Operation (R1 To R3)mentioning

confidence: 99%

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Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures

et al. 2022

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Light–dark cycles improve the 3HV molar fraction in PHBV produced by photoheterotrophic bacteria from odd‐chain fatty acids and cheese whey

Montiel‐Corona,

Puyol,

Buitrón

2024

J of Chemical Tech & Biotech

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BACKGROUNDPoly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHBV) is the most promising biopolymer for replacing petroleum‐derived plastics from polyhydroxyalkanoates (PHA) copolymers. The mechanical properties of PHBV improve as the content of the 3‐hydroxyvalerate monomer (3HV) increases. However, there is no information about the effect of light/dark cycles on PHA composition produced by phototrophic purple bacteria. This work aimed to improve the molar fraction of 3HV in the PHBV copolymer formed by a mixed phototrophic culture enriched in Rhodopseudomonas palustris, combining the effect of the illumination regime and using odd‐chain fatty acids and cheese whey as carbon sources.RESULTSUnder continuous lighting, the PHBV concentration (mg L−1) and 3HV molar fraction (%) were 508 ± 25 mg L−1 (1%), 193 ± 10 mg L−1 (22%), 252 ± 10 mg L−1 (1%), and 286 ± 10 mg L−1 (3%) for valerate, propionate, lactate, and fermented cheese whey, respectively. Interestingly, under light/dark cycles, the 3HV molar fraction increased 23‐fold when using valerate (light/dark cycle of 30 min), 12‐fold with lactate, and 1.5‐fold with fermented cheese whey. The PHBV content (% w/w) reached was 20–25%, 14–16%, 12–15%, and 8–12% for valerate, propionate, lactate, and fermented cheese whey, respectively.CONCLUSIONCombining odd‐chain fatty acids and light–dark cycles was an excellent strategy to improve the 3HV molar fraction in PHBV. This study shows for the first time that the 3HV molar fraction production is positively affected by light/dark cycles using odd‐chain fatty acids. Using light/dark cycles brings a double benefit: improving the molar fraction of 3HV in the polymer and decreasing the illumination‐associated cost. © 2024 Society of Chemical Industry (SCI).

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Microalgae-based domestic wastewater treatment: a review of biological aspects, bioremediation potential, and biomass production with biotechnological high-value

de Cassia Soares Brandão,

Oliveira,

dos Santos

et al. 2023

Environ Monit Assess

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Polyhydroxyalkanoates production from fermented domestic wastewater using phototrophic mixed cultures

Cited by 26 publications

References 28 publications

Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures

Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures

Light–dark cycles improve the 3HV molar fraction in PHBV produced by photoheterotrophic bacteria from odd‐chain fatty acids and cheese whey

Microalgae-based domestic wastewater treatment: a review of biological aspects, bioremediation potential, and biomass production with biotechnological high-value

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