Polyhydroxyalkanoates, Their Processing and Biomedical Applications

Asare, Emmanuel; Gregory, David A.; Fricker, Annabelle T. R.; Marcello, Elena; Paxinou, Alexandra; Taylor, Caroline S.; Haycock, John W.; Roy, Ipsita

doi:10.1201/9781003087663-14

Cited by 10 publications

(12 citation statements)

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“…Important parameters of cell matrices (scaffolds) are the development of the surface, including porosity, roughness, and hydrophilicity, which together determine adhesive properties, affecting the attachment, differentiation, and development of eukaryotic cells [ 72 ]. The influence of the composition of PHA and the methods of processing these polymers on the biological and functional properties of products made from them for biomedical purposes have been described by many authors [ 73 , 74 , 75 , 76 ].…”

Section: Resultsmentioning

confidence: 99%

Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646

et al. 2022

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Synthesis of P(3HB-co-3HV-co-4HV) copolymers by the wild-type strain Cupriavidus necator B-10646 on fructose or sodium butyrate as the main C-substrate with the addition of γ-valerolactone as a precursor of 3HV and 4HV monomers was studied. Bacterial cells were cultivated in the modes that enabled production of a series of copolymers with molar fractions of 3HV (from 7.3 to 23.4 mol.%) and 4HV (from 1.9 to 4.7 mol.%) with bacterial biomass concentration (8.2 ± 0.2 g/L) and PHA content (80 ± 2%). Using HPLC, DTA,DSC, X-Ray, SEM, and AFM, the physicochemical properties of copolymers and films prepared from them have been investigated as dependent on proportions of monomers. Copolymers are characterized by a reduced degree of crystallinity (Cx 38–49%) molecular weight characteristics Mn (45–87 kDa), and Mw (201–248 kDa) compared with P(3HB). The properties of the films surface of various composition including the porosity and surface roughness were studied. Most of the samples showed a decrease in the average pore area and an increase in their number with a total increase in 3HV and 4HV monomers. The results allow scaling up the productive synthesis of P(3HB-co-3HV-co-4HV) copolymers using Cupriavidus necator B-10646.

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Section: Resultsmentioning

confidence: 99%

Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646

et al. 2022

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“…For the final prototype we would envisage that the 3D printed scaffold would be topped with a randomly aligned electrospun or gyrospun fibrous mat, which would have fibre diameters of 100–150 nm, as has been performed in previous studies [ 15 , 145 ]. This is to mimic the topmost layer of the articular cartilage, where the fibres change direction to rest parallel to the surface of the articular cartilage, while maintaining the random fibre alignment in that plane [ 146 ].…”

Section: Resultsmentioning

confidence: 99%

“…The devices sold for clinical use include surgical films, meshes, plugs and patches, and are used for applications such as for the repair of inguinal hernias. PHAs are also being researched for a large number of further biomedical applications [ 15 ], including tissue repair patches [ 16 ], cardiac patches [ 17 ], cardiac valves [ 9 , 18 ], stents [ 19 ], nerve guide conduits [ 20 ], cartilage [ 21 ] and bone scaffolds [ 22 ], and drug carriers [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Additive Manufacturing of Polyhydroxyalkanoate-Based Blends Using Fused Deposition Modelling for the Development of Biomedical Devices

Gregory

Fricker

Mitrev

et al. 2023

JFB

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In the last few decades Additive Manufacturing has advanced and is becoming important for biomedical applications. In this study we look at a variety of biomedical devices including, bone implants, tooth implants, osteochondral tissue repair patches, general tissue repair patches, nerve guidance conduits (NGCs) and coronary artery stents to which fused deposition modelling (FDM) can be applied. We have proposed CAD designs for these devices and employed a cost-effective 3D printer to fabricate proof-of-concept prototypes. We highlight issues with current CAD design and slicing and suggest optimisations of more complex designs targeted towards biomedical applications. We demonstrate the ability to print patient specific implants from real CT scans and reconstruct missing structures by means of mirroring and mesh mixing. A blend of Polyhydroxyalkanoates (PHAs), a family of biocompatible and bioresorbable natural polymers and Poly(L-lactic acid) (PLLA), a known bioresorbable medical polymer is used. Our characterisation of the PLA/PHA filament suggest that its tensile properties might be useful to applications such as stents, NGCs, and bone scaffolds. In addition to this, the proof-of-concept work for other applications shows that FDM is very useful for a large variety of other soft tissue applications, however other more elastomeric MCL-PHAs need to be used.

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“…PHAs can be completely biodegraded by intra- or extracellular enzymes [ 5 , 6 , 7 ]. Their biodegradability [ 8 , 9 , 10 ] and biocompatibility properties allow them to be considered for a wide range of application areas such as environmental, medical, or pharmaceutical applications like drug delivery systems and tissue engineering [ 11 , 12 , 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Semi-Interpenetrating Materials Based on Poly(3-hydroxyalkanoate)s and Poly(ethyleneglycol) Diacrylate

Brelle

Anda

Ozturk

et al. 2022

Gels

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Biocompatible gels based on poly(3-hydroxyalkanoate)s (PHAs) were developed by radical polymerization in the presence of poly(ethylene glycol) diacrylate (PEGDA). In order to elaborate cross-linked networks based on PEGDA and PHAs, several PHAs were tested; saturated PHAs, such as poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) or poly(3-hydroxyoctanoate) (PHO), and an unsaturated PHA, poly(3-hydroxyoctanoate-co-3-hydroxyundecenoate) PHOU. The PHAxPEGDA1-x networks obtained in this work were studied by FTIR, Raman spectroscopy, DSC, TGA and NMR. The microscopic structure varied according to the mass proportions between the two polymers. Time Domain 1H DQ NMR measurements demonstrated that in the case of the unsaturated PHA, it was chemically crosslinked with PEGDA, due to the presence of double bonds in the lateral groups. The organogels were able to swell in organic solvents, such as THF, up to 2000% and in water up to 86%. It was observed by rheological analysis that the stiffness of the networks was dependent on the content of PHA and on the degree of cross-linking. The biocompatible characters of PHOU and PEGDA were not affected by the formation of the networks and these networks had the advantage of being non-cytotoxic to immortalized C2C12 myoblast cells.

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Polyhydroxyalkanoates, Their Processing and Biomedical Applications

Cited by 10 publications

References 1 publication

Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646

Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646

Additive Manufacturing of Polyhydroxyalkanoate-Based Blends Using Fused Deposition Modelling for the Development of Biomedical Devices

Biocompatible Semi-Interpenetrating Materials Based on Poly(3-hydroxyalkanoate)s and Poly(ethyleneglycol) Diacrylate

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