Polyhydroxybutyrate production by Cupriavidus necator in a corn biorefinery concept

Mello, Ariane Fátima Murawski de; Vandenberghe, Luciana Porto de Souza; Machado, Clara Matte Borges; Valladares-Diestra, Kim Kley; Carvalho, Júlio César de; Soccol, Carlos Ricardo

doi:10.1016/j.biortech.2022.128537

Cited by 15 publications

(2 citation statements)

References 39 publications

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“…Bioethanol production has emerged as a promising solution to mitigate these challenges by offering a renewable alternative to fossil fuels (Oyebanji and Kirikkaleli, 2022). Derived from organic matter such as biomass and agricultural residues, bioethanol presents multifaceted benefits including reduced greenhouse gas emissions, economic growth and enhanced energy security (Thiyagarajan et al, 2023;de Mello et al, 2023). The utilization of diverse feedstocks for bioethanol production, including cellulosic biomass, sugarcane and maize, underscores its flexibility and potential as a substitute for traditional fossil sources (Abdulsalam et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

From Trash to Treasure: The Prospect of Producing Bioethanol from Wastepaper through Pretreatment with Sulphuric Acid

Abdulsalam,

Mustapha,

Bolaji

et al. 2024

PHA

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Bioethanol is a prospective alternative to nonrenewable energy sources and this study aimed to produce bioethanol from waste paper using a pretreatment technique. Freshly fermented palm wine was analyzed using standard microbiological techniques to identify the microorganisms used in the study. The pre-treatment process utilized sulphuric acid and sodium hydroxide at varying concentrations (5%, 10%, 25%, 40% and 50%). The substrates had glucose concentrations ranging from 0.2 to 0.9 ppm and the peak yield was recorded at 10% sulphuric acid pretreatment. Bioethanol was produced through fractional distillation and sugar fermentation with Saccharomyces cerevisiae. The viability of bioethanol production using waste paper has been demonstrated as a sustainable method of waste management and a potential solution to energy shortages, particularly in developing countries.

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Section: Introductionmentioning

confidence: 99%

From Trash to Treasure: The Prospect of Producing Bioethanol from Wastepaper through Pretreatment with Sulphuric Acid

Abdulsalam,

Mustapha,

Bolaji

et al. 2024

PHA

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“…It has been shown to accumulate up to 90% of PHB based on dry weight under restricted nutrition [ 1 ]. Its broad carbon source utilization spectrum, detailed genomic information, and efficient gene editing system [ 2 , 3 ] position C. necator as a promising candidate for applications in environmental protection and bio-resource conversion [ 4 ]. Studies have shown that C. necator can utilize waste oil, lignocellulose-derived sugar, and inedible rice to produce PHB [ 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Optimizing Hexose Utilization Pathways of Cupriavidus necator for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions

Wang,

Luo,

Yao

et al. 2023

IJMS

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Cupriavidus necator is a versatile microbial chassis to produce high-value products. Blocking the poly-β-hydroxybutyrate synthesis pathway (encoded by the phaC1AB1 operon) can effectively enhance the production of C. necator, but usually decreases cell density in the stationary phase. To address this problem, we modified the hexose utilization pathways of C. necator in this study by implementing strategies such as blocking the Entner–Doudoroff pathway, completing the phosphopentose pathway by expressing the gnd gene (encoding 6-phosphogluconate dehydrogenase), and completing the Embden–Meyerhof–Parnas pathway by expressing the pfkA gene (encoding 6-phosphofructokinase). During heterotrophic fermentation, the OD600 of the phaC1AB1-knockout strain increased by 44.8% with pfkA gene expression alone, and by 93.1% with gnd and pfkA genes expressing simultaneously. During autotrophic fermentation, gnd and pfkA genes raised the OD600 of phaC1AB1-knockout strains by 19.4% and 12.0%, respectively. To explore the effect of the pfkA gene on the production of C. necator, an alanine-producing C. necator was constructed by expressing the NADPH-dependent L-alanine dehydrogenase, alanine exporter, and knocking out the phaC1AB1 operon. The alanine-producing strain had maximum alanine titer and yield of 784 mg/L and 11.0%, respectively. And these values were significantly improved to 998 mg/L and 13.4% by expressing the pfkA gene. The results indicate that completing the Embden–Meyerhof–Parnas pathway by expressing the pfkA gene is an effective method to improve the growth and production of C. necator.

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From Knallgas Bacterium to Promising Biomanufacturing Host: The Evolution of Cupriavidus necator

Casey,

Diaz-Garcia,

et al. 2024

Advances in Biochemical Engineering/Biotechnology

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Polyhydroxybutyrate production by Cupriavidus necator in a corn biorefinery concept

Cited by 15 publications

References 39 publications

From Trash to Treasure: The Prospect of Producing Bioethanol from Wastepaper through Pretreatment with Sulphuric Acid

From Trash to Treasure: The Prospect of Producing Bioethanol from Wastepaper through Pretreatment with Sulphuric Acid

Optimizing Hexose Utilization Pathways of Cupriavidus necator for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions

From Knallgas Bacterium to Promising Biomanufacturing Host: The Evolution of Cupriavidus necator

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