Polyketides and Polyketide‐Containing Glycolipids of<i>Mycobacterium tuberculosis</i>: Structure, Biosynthesis and Biological Activities

Guilhot, Christophe; Daffé, Mamadou

doi:10.1002/9783527611614.ch2

Cited by 5 publications

(1 citation statement)

References 77 publications

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“…Rv1180/Pks3 and Rv1181/Pks4 (also known as Msl3) comprise a polyketide synthase needed to synthesize the mycolipenic side chains of PAT. 48,49 Interestingly, PAT is a surface lipid found only in pathogenic mycobacteria, 49,50 but the explanation for the PAT deficiency in BCG Pasteur has remained unknown. 3 Our data suggest that it is due to deficiency in the PAT biosynthetic machinery.…”

Section: Journal Of Proteome Researchmentioning

confidence: 99%

Comparison of the Membrane Proteome of Virulent Mycobacterium tuberculosis and the Attenuated Mycobacterium bovis BCG Vaccine Strain by Label-Free Quantitative Proteomics

et al. 2013

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The Mycobacterium tuberculosis (MTB) membrane is rich in antigens that are potential targets for diagnostics and the development of new vaccines. To better understand the mechanisms underlying MTB virulence and identify new targets for therapeutic intervention we investigated the differential composition of membrane proteomes between virulent M. tuberculosis H37Rv (MTB) and the Mycobacterium bovis BCG vaccine strain. To compare the membrane proteomes, we used LC-MS/MS analysis in combination with label-free quantitative (LFQ) proteomics, utilizing the area-under-curve (AUC) of the extracted ion chromatograms (XIC) of peptides obtained from m/z and retention time alignment of MS1 features. With this approach, we obtained relative abundance ratios for 2,203 identified membrane-associated proteins in high confidence. Of these proteins, 294 showed statistically significant differences of at least 2 fold, in relative abundance between MTB and BCG membrane fractions. Our comparative analysis detected several proteins associated with known genomic regions of difference between MTB and BCG as being absent, which validated the accuracy of our approach. In further support of our label-free quantitative data, we verified select protein differences by immunoblotting. To our knowledge we have generated the first comprehensive and high coverage profile of comparative membrane proteome changes between virulent MTB and its attenuated relative BCG, which helps elucidate the proteomic basis of the intrinsic virulence of the MTB pathogen.

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Section: Journal Of Proteome Researchmentioning

confidence: 99%

Comparison of the Membrane Proteome of Virulent Mycobacterium tuberculosis and the Attenuated Mycobacterium bovis BCG Vaccine Strain by Label-Free Quantitative Proteomics

et al. 2013

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Lipid and Lipoarabinomannan Isolation and Characterization

Daffé

Lanéelle

Spina

et al. 2021

Methods in Molecular Biology

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Lipid and Lipoarabinomannan Isolation and Characterization

Lanéelle

Nigou

Daffé

2015

Methods in Molecular Biology

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Mycobacteria are microorganisms that contain a very high content of structurally diverse lipids, some of them being biologically active substances. As such the lipid composition is commonly used to characterize mycobacterial strains at the species and type-species level. This chapter describes the methods that allow the purification of the most commonly isolated biologically active lipids and those used for analyzing extractable lipids and their constituents, cell wall-linked mycolic acids and lipoarabinomannan (LAM). The latter involve simple chromatographic and analytical techniques, such as thin-layer chromatography and gas chromatography coupled to mass spectrometry.

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Polyketides and Polyketide‐Containing Glycolipids ofMycobacterium tuberculosis: Structure, Biosynthesis and Biological Activities

Cited by 5 publications

References 77 publications

Comparison of the Membrane Proteome of Virulent Mycobacterium tuberculosis and the Attenuated Mycobacterium bovis BCG Vaccine Strain by Label-Free Quantitative Proteomics

Comparison of the Membrane Proteome of Virulent Mycobacterium tuberculosis and the Attenuated Mycobacterium bovis BCG Vaccine Strain by Label-Free Quantitative Proteomics

Lipid and Lipoarabinomannan Isolation and Characterization

Lipid and Lipoarabinomannan Isolation and Characterization

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