Polylysine Crosslinked AIE Dye Based Fluorescent Organic Nanoparticles for Biological Imaging Applications

Liu, Meiying; Zhang, Xiqi; Yang, Bin; Liu, Liangji; Deng, Fengjie; Zhang, Xiaoyong; Wei, Yen

doi:10.1002/mabi.201400140

Cited by 42 publications

(33 citation statements)

References 74 publications

(92 reference statements)

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“…[1][2][3][4][5][6][7][8][9] Especially fluorescent organic nanoparticles (FONs) have aroused considerable interest in biological applications because of their biocompatibility, rich chemical modification, biodegradability, and high fluorescence quantum yield (FQY). [10][11][12][13][14][15] FONs, such as fluorescent macromolecules, fluorescent polymeric nanoparticles, and fluorescent supermacromolecular nanoassemblies, have been modified for further conjugation with biomolecules and/or fluorophores. [16,17] Methods such as living radical polymerization (LRP) to form core-shell structures or attaching fluorescent dyes in polymeric spheres have been reported.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Amphiphilic Hyperbranched AIE‐active Fluorescent Organic Nanoparticles and Their Application in Biological Application

Wang

Xü

et al. 2015

Macromolecular Bioscience

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Aggregation-induced emission (AIE) dyes have recently attracted much attention for biomedical applications for their remarkable AIE properties. However, the hydrophobic nature of AIE dyes made them difficult to be dispersed in physiological solution and problematic for biomedical application directly. Great efforts have been made to overcome this problem, and different strategies for preparation of water dispersible AIE based nanoprobes had been explored previously. However, a facile and effective strategy is still highly desirable and of great importance for the biomedical applications of AIE dye based on nanoprobes. In this work, the fabrication of amphiphilic hyperbranched fluorescent organic nanoparticles with a core-shell structure based on an AIE dye [tetraphenylethene acrylate (TPE-O-E)] and a hyperbranched polyamino compound [polyethylene imine (PEI)] through Michael addition reaction is described for the first time. The AIE dye as well as the final product PEI-TPE-O-E was characterized in detail by a number of techniques. To test their biomedical application potential, the cell viability as well as cell imaging properties of the PEI-TPE-O-E was also examined. The results showed that the PEI-TPE-O-E organic nanoparticles presented high water dispersiblity, ultrabright fluroerescence, low cytotoxicity and excellent biocompatibility, making them promising for biological imaging and gene delivery applications.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Amphiphilic Hyperbranched AIE‐active Fluorescent Organic Nanoparticles and Their Application in Biological Application

Wang

Xü

et al. 2015

Macromolecular Bioscience

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“…Silole fluorogens are among the most prominent AIE dyes, and BODIPY-based dyes with AIE properties have also been described to exhibit shifted photoluminescence spectra depending on solvent polarity [336]. Multiple fluorescent AlE-dye labeled polymeric NPs have been described [337–340], often with facile synthesis in one-pot reaction schemes [341–343], and such materials have been used for intravital two-photon imaging of vasculature [344]. Relatively broad excitation and emission spectra represent a substantial limitation of this approach, however.…”

Section: The Nuts and Bolts Of Ivm Nanoparticle Imagingmentioning

confidence: 99%

Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior

Miller

Weissleder

2017

Advanced Drug Delivery Reviews

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Therapeutic nanoparticles (NPs) can deliver cytotoxic chemotherapeutics and other drugs more safely and efficiently to patients; furthermore, selective delivery to target tissues can theoretically be accomplished actively through coating NPs with molecular ligands, and passively through exploiting physiological “enhanced permeability and retention” features. However, clinical trial results have been mixed in showing improved efficacy with drug nano-encapsulation, largely due to heterogeneous NP accumulation at target sites across patients. Thus, a clear need exists to better understand why many NP strategies fail in vivo and not result in significantly improved tumor uptake or therapeutic response. Multicolor in vivo confocal fluorescence imaging (intravital microscopy; IVM) enables integrated pharmacokinetic and pharmacodynamic (PK/PD) measurement at the single-cell level, and has helped answer key questions regarding the biological mechanisms of in vivo NP behavior. This review summarizes progress to date and also describes useful technical strategies for successful IVM experimentation.

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“…The physical method is mainly to encapsulate the AIE dyes with biocompatible amphiphilic polymers to afford FPNs; [25][26][27] nevertheless, dye leakage or surface coating detachment is the main obstacle in this non-covalent system. 20,[34][35][36] Glycidyl methacrylate (GM) is an attractive monomer due to its economic and biological value. [19][20][21]30,31 Despite many impressive advances in fabricating AIE based macromolecules, more versatile and robust strategies are still highly demanded.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of cross-linked fluorescent polymer nanoparticles and their cell imaging applications

et al. 2015

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Novel aggregation induced emission (AIE) dye based cross-linked amphiphilic fluorescent polymers have been prepared facilely by a one pot method. This was carried out first by free radical polymerization between the AIE monomer (PhE) and glycidyl methacrylate (GM), then by the ring-opening reaction between GM and polyethyleneimine (PEI) to obtain the cross-linked polymer. The resultant cross-linked amphiphilic polymer was prone to self-assemble into stable nanoparticles with high water dispersibility due to the surplus amino groups and hydroxyl groups covered on the surface, which can also be further functionalized. The thus obtained nanoparticles demonstrated strong orange fluorescent emission with a quantum yield of about 41% owing to the AIE dyes in the cores of the nanoparticles. Biocompatibility evaluation and cell uptake behaviour of the nanoparticles were further investigated to explore their potential biomedical applications and the demonstrated excellent biocompatibility made them promising for cell imaging. † Electronic supplementary information (ESI) available: Detailed information about CMC, hydrodynamic size changes and photostability of PhE-GM-PEI FPNs; GPC traces of PhE-GM-PEI (eluent: DMF). See

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Polylysine Crosslinked AIE Dye Based Fluorescent Organic Nanoparticles for Biological Imaging Applications

Cited by 42 publications

References 74 publications

Synthesis of Amphiphilic Hyperbranched AIE‐active Fluorescent Organic Nanoparticles and Their Application in Biological Application

Synthesis of Amphiphilic Hyperbranched AIE‐active Fluorescent Organic Nanoparticles and Their Application in Biological Application

Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior

Fabrication of cross-linked fluorescent polymer nanoparticles and their cell imaging applications

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