1998
DOI: 10.1006/jmbi.1998.1830
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Polymer chain statistics and conformational analysis of DNA molecules with bends or sections of different flexibility

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Cited by 274 publications
(307 citation statements)
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“…4. The values for (dT) 30 , (dT) 40 , and (dT) 50 match each other and are larger than previous results between 0.75 and 4 nm (28,29,(31)(32)(33) (variations primarily due to differences in buffers and sequence). In theoretical models, l P is divided into intrinsic l P 0 and electrostatic l P el components, l P ϭ l P 0 ϩ l P el .…”
Section: And 4; See Sample Preparation and Characterization Inmentioning
confidence: 36%
“…4. The values for (dT) 30 , (dT) 40 , and (dT) 50 match each other and are larger than previous results between 0.75 and 4 nm (28,29,(31)(32)(33) (variations primarily due to differences in buffers and sequence). In theoretical models, l P is divided into intrinsic l P 0 and electrostatic l P el components, l P ϭ l P 0 ϩ l P el .…”
Section: And 4; See Sample Preparation and Characterization Inmentioning
confidence: 36%
“…Local changes in rigidity, curvature and/or topology of a DNA molecule induced by chemical compounds or by DNA binding proteins [7,8,9,10,11,12] have been also studied. However, the three dimensional conformation of a flexible biopolymer is not directly delivered by the AFM, but we show here that such information can be extracted from the AFM images.…”
mentioning
confidence: 99%
“…This occurs even though the sample is deposited and imaged entirely under buffer. This clearly demonstrates the ability of the surface to change the structure of DNA in addition to its well-known effect on the polymer chain statistics (6,7). The influence of surface binding is clearly the major experimental issue confronting microscopy at the molecular level, and one that AFM is ideally suited to investigate, given the simplicity of sample preparation and the ability to image in situ.…”
Section: Discussionmentioning
confidence: 99%
“…Rivetti et al (6,7) have studied the change in chain statistics that occurs as a molecule becomes trapped at a solid-liquid interface, while Fang et al showed how DNA dried onto mica after deposition from ethanol/water mixtures (8) exists in a mixture of B-and A-forms. There has not, as yet, been a clear demonstration of an internal conformational transition induced by surface effects alone.…”
Section: Introductionmentioning
confidence: 99%