Polymer Complexes in Biological Applications

Hedrich, Jana; Wu, Yuzhou; Kuan, Seah Ling; Kuehn, Frauke; Pietrowski, Eweline; Sahl, Mike; Muth, Sandra; Müllen, Kläus; Luhmann, Heiko J.; Weil, Tanja; Schmidt, Manfred

doi:10.1007/12_2013_229

Cited by 1 publication

(1 citation statement)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is in contrast to the results obtained in high protein media where no knock-down of ApoB100 was detectable. Since it is well-known that siRNA and DNA may be released from polyelectrolyte complexes by a large excess of albumin, , PAGE was conducted on siRNA–brush complexes incubated with increasing concentrations of albumin. As shown in the Supporting Information, Figure S20, albumin concentrations as high as 40% (w/w) could not release siRNA out of the complex.…”

Section: Resultsmentioning

confidence: 99%

Cylindrical Brush Polymers with Polysarcosine Side Chains: A Novel Biocompatible Carrier for Biomedical Applications

et al. 2015

Self Cite

View full text Add to dashboard Cite

Cylindrical brush polymers constitute promising polymeric drug delivery systems (nanoDDS). Because of the densely grafted side chains such structures may intrinsically exhibit little protein adsorption (“stealth” effect) while providing a large number of functional groups accessible for bioconjugation reactions. Polysarcosine (PSar) is a highly water-soluble, nonionic and nonimmunogenic polypeptoid based on the endogenous amino acid sarcosine (N-methyl glycine). Here we report on the synthesis, characterization and biocompatibility of cylindrical brush polymers with either polysarcosine side chains or poly-l-lysine-b-polysarcosine side chains. The latter leads to block copolypept(o)id based core–shell cylindrical brushes with a cationic poly-l-lysine (PLL) core and a neutral polysarcosine corona. The cylindrical brush polymers were prepared by ring-opening polymerization of the respective N-carboxyanhydrides (NCA) from a macroinitiator chain. Preliminary experiments on complex formation with siRNA demonstrate that a core–shell cylindrical brush polymer may complex on average up to 270 RNA molecules amounting to a high loading efficiency of N+/P– = 1.1. No bridging between cylindrical brushes leading to larger aggregates is observed. In vitro studies on the silencing of the expression of ApoB100, which is abundantly expressed in AML-12 hepatocytes, induced by siRNA-cylindrical core–shell brush complexes showed high efficiency, leading to a knock-down efficiency of ApoB100 mRNA of 70%.

show abstract

Section: Resultsmentioning

confidence: 99%