Polymer-dispersed reduced graphene oxide nanosheets and Prussian blue modified biosensor for amperometric detection of sarcosine

Rajarathinam, Thenmozhi; Kwon, Minho; Thirumalai, Dinakaran; Kim, Seonghye; Lee, Seulah; Yoon, Jung‐Sik; Paik, Hyun‐jong; Kim, Suhkmann; Lee, Jaewon; Ha, Hong Koo; Chang, Seung‐Cheol

doi:10.1016/j.aca.2021.338749

Cited by 33 publications

(14 citation statements)

References 37 publications

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“…Qamar et al reported a non-ionic block copolymer (Lugalvan BNO12) for dispersing graphene in aqueous solutions [21]. Our group also reported the dispersion of graphene in aqueous solutions using block copolymers such as poly(sodium 4-styrene sulfonate-r-LAHEMA) to prepare a sarcosine biosensor [22].…”

Section: Introductionmentioning

confidence: 69%

Disposable Voltammetric Sensor Modified with Block Copolymer-Dispersed Graphene for Simultaneous Determination of Dopamine and Ascorbic Acid in Ex Vivo Mouse Brain Tissue

et al. 2021

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Dopamine (DA) and ascorbic acid (AA) are two important biomarkers with similar oxidation potentials. To facilitate their simultaneous electrochemical detection, a new voltammetric sensor was developed by modifying a screen-printed carbon electrode (SPCE) with a newly synthesized block copolymer (poly(DMAEMA-b-styrene), PDbS) as a dispersant for reduced graphene oxide (rGO). The prepared PDbS–rGO and the modified SPCE were characterized using a range of physical and electrochemical techniques including Raman spectroscopy, scanning electron microscopy, transmission electron microscopy, cyclic voltammetry, electrochemical impedance spectroscopy, and linear sweep voltammetry. Compared to the bare SPCE, the PDbS–rGO-modified SPCE (PDbS–rGO/SPCE) showed better sensitivity and peak-to-peak separation for DA and AA in mixed solutions. Under the optimum conditions, the dynamic linear ranges for DA and AA were 0.1–300 and 10–1100 µM, and the detection limits were 0.134 and 0.88 µM (S/N = 3), respectively. Furthermore, PDbS–rGO/SPCE exhibited considerably enhanced anti-interference capability, high reproducibility, and storage stability for four weeks. The practical potential of the PDbS–rGO/SPCE sensor for measuring DA and AA was demonstrated using ex vivo brain tissues from a Parkinson’s disease mouse model and the control.

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Section: Introductionmentioning

confidence: 69%

Disposable Voltammetric Sensor Modified with Block Copolymer-Dispersed Graphene for Simultaneous Determination of Dopamine and Ascorbic Acid in Ex Vivo Mouse Brain Tissue

et al. 2021

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“…Approximately 6 µL of the prepared LOXENs was drop-casted onto the SPCE surface. Prior to drop-casting, the SPCE was modified with the nanozyme ( Section S1 in Supplementary material ) following our previous report [ 31 ]. The obtained biosensors, LOXENs/PB/SPCEs, were finally dried at 4 °C for 12 h before use.…”

Section: Methodsmentioning

confidence: 99%

Robust Nanozyme-Enzyme Nanosheets-Based Lactate Biosensor for Diagnosing Bacterial Infection in Olive Flounder (Paralichthys olivaceus)

et al. 2021

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Bacterial infections in fish farms increase mass mortality and rapid detection of infection can help prevent its widespread. Lactate is an important biomarker for early diagnosis of bacterial infections in farmed olive flounder (Paralichthys olivaceus). To determine the lactate levels, we designed a disposable amperometric biosensor based on Prussian blue nanozyme and lactate oxidase (LOX) entrapped in copolymer-reduced graphene oxide (P-rGO) on screen-printed carbon electrodes. Because LOX is inherently unstable, P-rGO nanosheets were utilized as a base matrix to immobilize it. After optimization in terms of enzyme loading, operating potential, and pH, the biosensor displayed maximum current responses within 5 s at the applied potential of –0.1 V vs. internal Ag/AgCl. The biosensor had Langmuir-type response in the lactate concentration range from 10 µM to 1.6 mM, a dynamic linear response range of 10–100 µM, a sensitivity of 15.9 µA mM−1 cm−2, and a lower detection limit of 3.1 µM (S/N = 3). Additionally, the biosensor featured high reproducibility, good selectivity, and stability till four weeks. Its practical applicability was tested in olive flounder infected by Streptococcus parauberis against the uninfected control. The results were satisfactory compared to those of a standard colorimetric assay kit, validating our method.

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“…[24] During the fabrication of FET-based chemiresistive biosensors, the semiconducting sensing interface usually needs to be functionalized for the immobilization of various biorecognition elements. [25] Consequently, abundant carboxyl groups can be uniformly modified on the semiconducting sensing interface based on the 𝜋-𝜋 stacking interaction between the six-membered ring of graphene and the substance, [26][27][28] which can be expected to supply a large number of evenly distributed functional groups for the immobilization of enzymes.…”

Section: Introductionmentioning

confidence: 99%

A Flexible and Wearable Chemiresistive Biosensor Fabricated by Laser Inducing for Real‐Time Glucose Analysis of Sweat

Sun

Song

Zhao

et al. 2023

Adv Materials Inter

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In this study, a flexible and wearable chemiresistive biosensor (FWCB) is developed for the real‐time analysis of glucose in sweat on the human skin surface based on a novel detection strategy of p‐type reduced graphene oxide (rGO) sensing film, which met the requirements of rapid, nondestructive testing. The proposed FWCB is fabricated in the form of interdigital electrodes (IEs) made of laser‐induced graphene (LIG) synthesized by the laser inducing of a polyimide (PI) film. Additionally, a semiconducting rGO sensing film modified on the surface of IEs is synthesized by thermal reduction of graphene oxide (GO), which is functionalized with glucose oxidase (GOx) by chemical cross‐linking to obtain GOx/FWCB. Moreover, the key parameters for FWCB fabrication are optimized, and the sensing strategy of the proposed GOx/FWCB is also investigated. The results show that the proposed GOx/FWCB can be used for the detection of glucose in the range of 0.01–3.0 mM with satisfactory selectivity, and the limit of detection (LOD) is calculated to be as low as 0.8 µM (S/N = 3). These dramatic advantages endow the proposed FWCB with broad application prospects in the field of portable, wearable, and real‐time detection of glucose in human sweat for health monitoring.

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Polymer-dispersed reduced graphene oxide nanosheets and Prussian blue modified biosensor for amperometric detection of sarcosine

Cited by 33 publications

References 37 publications

Disposable Voltammetric Sensor Modified with Block Copolymer-Dispersed Graphene for Simultaneous Determination of Dopamine and Ascorbic Acid in Ex Vivo Mouse Brain Tissue

Disposable Voltammetric Sensor Modified with Block Copolymer-Dispersed Graphene for Simultaneous Determination of Dopamine and Ascorbic Acid in Ex Vivo Mouse Brain Tissue

Robust Nanozyme-Enzyme Nanosheets-Based Lactate Biosensor for Diagnosing Bacterial Infection in Olive Flounder (Paralichthys olivaceus)

A Flexible and Wearable Chemiresistive Biosensor Fabricated by Laser Inducing for Real‐Time Glucose Analysis of Sweat

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