2024
DOI: 10.1101/2024.01.22.576751
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Polymer-extracted structure of the mechanosensitive channel MscS reveals the role of protein-lipid interactions in the gating cycle

Elissa Moller,
Madolyn Britt,
Fei Zhou
et al.

Abstract: Membrane protein structure determination is not only technically challenging but is further complicated by the removal or displacement of lipids, which can result in non-native conformations or a strong preference for certain states at the exclusion of others. This is especially applicable to mechanosensitive channels (MSCs) that evolved to gate in response to subtle changes in membrane tension, such as MscS, a model bacterial system for MSC gating with homologs found across all phyla of walled organisms. MscS… Show more

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“…We could not obtain samples of sufficient amounts and quality to perform high-resolution studies of MsbA with these polymer-based membrane mimics. Further optimization is needed to achieve samples compatible with high-resolution cryo-EM reconstructions of ABC transporters 35 .…”
Section: Discussionmentioning
confidence: 99%
“…We could not obtain samples of sufficient amounts and quality to perform high-resolution studies of MsbA with these polymer-based membrane mimics. Further optimization is needed to achieve samples compatible with high-resolution cryo-EM reconstructions of ABC transporters 35 .…”
Section: Discussionmentioning
confidence: 99%