Polymer Material Design by Microfluidics Inspired by Cell Biology and Cell‐Free Biotechnology

Thiele, Julian

doi:10.1002/macp.201600429

Cited by 21 publications

(11 citation statements)

References 130 publications

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“…11,12 Exploiting these advantages, engineered microparticles with controlled sizes, monodispersity, diverse morphologies, and specific functions can be generated, and are playing an increasingly important role in biomedical fields. 5,13 For instance, as drug delivery vehicles, 6,14,15 microcapsules or multi-core microparticles can be prepared with well-defined structures and compositions that allow for high encapsulation efficiency and well-controlled release of the encapsulants. As cell carriers, 16 hydrogel microparticles can be produced to act as extracellular matrix (ECM) to protect cells from the surrounding environment and maintain efficient nutrient and metabolic exchanges for long term cell culture.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic fabrication of microparticles for biomedical applications

et al. 2018

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Droplet microfluidics offers exquisite control over the flows of multiple fluids in microscale, enabling fabrication of advanced microparticles with precisely tunable structures and compositions in a high throughput manner. The combination of these remarkable features with proper materials and fabrication methods has enabled high efficiency, direct encapsulation of actives in microparticles whose features and functionalities can be well controlled. These microparticles have great potential in a wide range of bio-related applications including drug delivery, cell-laden matrices, biosensors and even as artificial cells. In this review, we briefly summarize the materials, fabrication methods, and microparticle structures produced with droplet microfluidics. We also provide a comprehensive overview of their recent uses in biomedical applications. Finally, we discuss the existing challenges and perspectives to promote the future development of these engineered microparticles.

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Section: Introductionmentioning

confidence: 99%

Microfluidic fabrication of microparticles for biomedical applications

et al. 2018

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“…Many approaches have been developed to fabricate hollow microcapsules, such as layer-by-layer technique, [10,11] microfluidics, [12][13][14] template methods, [15,16] suspen sion polymerization [17][18][19] etc. However, it proves to be difficult to obtain hollow microcapsules with controllable mechanical properties merely by the methods mentioned above.…”

Section: Doi: 101002/macp201800395mentioning

confidence: 99%

Hollow Microcapsules with Controlled Mechanical Properties Templated from Pickering Emulsion Droplets

Wang

Chen

Sun

et al. 2019

Macro Chemistry & Physics

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Hollow microcapsules with controlled mechanical strength are obtained from Pickering emulsion templates. The shell of the hollow microcapsules comprising UV‐curable resin and reactive silica nanoparticles enables tuning of the mechanical properties in a controlled manner. The results show that the particle stabilizer can be packed regularly on the oil–water interface and the shell thickness of microcapsules can be increased to 1–2 µm compared with traditional preparation methods. Hollow particles with thicker walls can prevent collapse and breakage. In this work, a nanoindentation device is used to measure the mechanical properties of hollow particles, and the results show a clear trend of reduced modulus and hardness decreases with increasing ratio of prepolymers in the reaction mixture. When the mass of PUA varies from 10% to 30%, the hardness decreases from 0.09 to 0.04 GPa, while the reduced modulus decreases from 0.17 to 0.06 GPa. The control of mechanical properties of the microcapsule can enhance its uses in conventional areas as well as in new fields such as precise micro force sensors etc.

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“…Characterization using light microscopy during the formation of prepolymer droplets helped to identify any irregularities in shape or size, following which the T-junction microfluidic device was washed, set up again, and then the protocol restarted to ensure the collected prepolymer droplets were up to the standard. It was crucial to let the continuous phase flow first through the set-up to ensure there was no presence of oxygen, as it can inhibit free-radical photopolymerization and promote formation of heterogeneous polymer networks (Thiele 2017). Once homogeneous prepolymer droplets were identified, they were collected in several falcon tubes until the continuous phase, more likely, or the discontinuous phase, less likely, ran out from the syringes.…”

Section: Cryogel Microcarrier Synthesis and Characterizationmentioning

confidence: 99%

Synthesis and Characterization of cryogel microcarriers for sustained local delivery of anticancer therapeutics to Glioblastoma multiforme

Azhar¹

2019

Preprint

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Poly(ethylene glycol) diacrylate (PEGDA)-based cryogel microcarriers incorporated with variable proportion of 3-Sulpho-propyl acrylate (SPA) were synthesized to deliver the broad-spectrum anticancer drug, doxorubicin, in a controlled and sustained method across the blood-brain barrier (BBB) for treatment of glioblastoma multiforme (GBM). Combination of these two polymeric materials was intended to allow the delivery of doxorubicin molecules through the brain parenchyma without prompting multidrug resistance mechanism by the BBB. Due to it its binding affinity to protonated doxorubicin molecules, molar percentage of incorporated SPA into the cryogel microcarriers was hypothesized to be proportional to the level of doxorubicin uptake. To enable droplet formation, fluorinated oil with perfluoropolyether surfactant served as the continuous phase. The prepolymer droplets were fabricated using a T-junction microfluidic device and were crosslinked via photopolymerization. Light microscopy was used for characterization of the microcarriers before and after cryogelation, with regards to size and shape. Doxorubicin loading and release studies were performed to evaluate drug elution rates for each type of microcarrier suspension. Loading of cryogel microcarriers was done at room temperature for a period of 7 days whereas the release study was carried out at 37°C in an incubator for 28 days. Significant differences (p < 0.0001) in uptake and release of doxorubicin, compared to the control, were seen in all SPA incorporated cryogels while those cryogels without any proportion of SPA incorporation had no significant difference ( p > 0.05). Cryogel suspensions with a SPA to PEGDA molar percentage of 60% or less were coherent with the proposed hypothesis, however, microcarriers that had a higher proportion of SPA did not seem to follow this trend. In conclusion, doxorubicin loading and release in cryogel microcarriers is not entirely dependent on its binding affinity with available SPA functional groups, but also on other physiological and mechanical parameters as well.

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Polymer Material Design by Microfluidics Inspired by Cell Biology and Cell‐Free Biotechnology

Cited by 21 publications

References 130 publications

Microfluidic fabrication of microparticles for biomedical applications

Microfluidic fabrication of microparticles for biomedical applications

Hollow Microcapsules with Controlled Mechanical Properties Templated from Pickering Emulsion Droplets

Synthesis and Characterization of cryogel microcarriers for sustained local delivery of anticancer therapeutics to Glioblastoma multiforme

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