Polymer modification of lipases, substrate interactions, and potential inhibition

Advances in Thermoresponsive Polymers

“…For α-chymotrypsin, the widely applied method for polymer conjugation with epoxy-functional polymers involves amination of the epoxy group with a diamine followed by coupling the resulting amine-functionalized polymer to the enzyme by glutaraldehyde [114,115]. Although poly(N-isopropylacrylamide) bioconjugates have been investigated in numerous cases, its relatively large extent of hysteresis due to hydrogen bonding between PNIPAAm chains [46,47] and even full activity loss of the conjugated enzyme [122] may limit its application possibilities. In contrast, the lack of hydrogen bonding between PDEAAm chains may provide unique advantages for bioconjugations with functional PDEAAm.…”