Polymer solutions and entropic‐based systems for double‐stranded DNA capillary electrophoresis and microchip electrophoresis

Xu, Feng; Baba, Yoshinobu

doi:10.1002/elps.200405923

Cited by 53 publications

(33 citation statements)

References 177 publications

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“…System integration led to the 'lab-on-a-chip' concept with such multiple functions as PCR amplification, restriction digestion, separation, fraction collection, etc [10]. The progress in the field was summarized in several reviews in the past few years [9,[11][12][13][14][15][16][17][18][19][20][21].…”

Section: Genome Variabilitymentioning

confidence: 99%

Genotyping with microfluidic devices

Szantai

Guttman

2006

Electrophoresis

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In the past few years, electrophoresis microchips have been increasingly utilized to interrogate genetic variations in the human and other genomes. Microfluidic devices can be readily applied to speed up existing genotyping protocols, in particular the ones that require electric field-mediated separations in conjunction with restriction fragment analysis, DNA sequencing, hybridization-based techniques, allele-specific amplification, heteroduplex analysis, just to list the most important ones. As a result of recent developments, microfabricated electrophoresis devices offer several advantages over conventional slab-gel electrophoresis, such as small sample volume requirement, low reagent consumption, the option of system integration and easy multiplexing. The analysis speed of microchip electrophoresis is significantly higher than that of any other electric field-mediated separation techniques. State-of-the-art microfluidic bioanalytical devices already claim their place in most molecular biology laboratories. This review summarizes the recent developments in microchip electrophoresis methods of nucleic acids, particularly for rapid genotyping, that will most likely play a significant role in the future of clinical diagnostics.

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Section: Genome Variabilitymentioning

confidence: 99%

Genotyping with microfluidic devices

Szantai

Guttman

2006

Electrophoresis

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“…Fluidic chips are used in many fields, [1][2][3][4][5][6] such as chemistry and biology. 3D fluidic chips are presently attracting much attention because they can accommodate more channels.…”

Section: Introductionmentioning

confidence: 99%

Low-cost Methods for Making 3D Fluidic Polymer and Glass Chips Using Metal Templates

et al. 2015

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“…[37][38][39][40][41][42] These make the low viscous separation Medias (less than few hundreds cP) very essential for chip separation. 43) Several low viscous separation media have been studied for DNA separation on chip.…”

Section: Introductionmentioning

confidence: 99%

Low Viscous Separation Media for Genomics and Proteomics Analysis on Microchip Electrophoresis System

Jabasini

Murakami

Kaji

et al. 2006

Biological & Pharmaceutical Bulletin

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Microchip electrophoresis has widely grown during the past few years, and it has showed a significant result as a strong separation tool for genomic as well as proteomic researches. To enhance and expand the role of microchip electrophoresis, several studies have been proposed especially for the low viscous separation media, which is an important factor for the success of microchip with its narrow separation channels. In this paper we show an overview for the done researches in the field of low viscous media developed for the use in microchip electrophoresis. For genomic separation studies polyhydroxy additives have been used enhance the separation of DNA at low polymer concentration of HPMC (Hydroxypropylmethyl cellulose) which could keep the viscosity low. Mixtures of poly(ethylene oxide) as well as Hydroxyporpyl cellulose have been successfully introduced for chip separation. Furthermore high molecular mass polyacrylamides at low concentrations have been studied for DNA separation. A mixture of polymer nanoparticle with conventional polymers could show a better resolution for DNA at low concentration of the polymer. For the proteomic field isoelectric focusing on chip has been well overviewed since it is the most viscous separation media which is well used for the protein separation. The different types of isoelectric focusing such as the ampholyte-free type, the thermal type as well as the ampholyte-depended type have been introduced in this paper. Isoelectric focusing on chip with its combination with sodium dodecyl sulfate (SDS) page or free solution could give a better separation. Several application for this low viscous separation medias for either genomic or proteomic could clearly show the importance of this field.

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Polymer solutions and entropic‐based systems for double‐stranded DNA capillary electrophoresis and microchip electrophoresis

Cited by 53 publications

References 177 publications

Genotyping with microfluidic devices

Genotyping with microfluidic devices

Low-cost Methods for Making 3D Fluidic Polymer and Glass Chips Using Metal Templates

Low Viscous Separation Media for Genomics and Proteomics Analysis on Microchip Electrophoresis System

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