Polymer ultrastructure governs AA9 lytic polysaccharide monooxygenases functionalization and deconstruction efficacy on cellulose nano-crystals

Magri, Silvia; Nazerian, Gulsen; Segato, Tiriana; Monclaro, Antonielle Vieira; Zarattini, Marco; Segato, Fernando; Polikarpov, Igor; Cannella, David

doi:10.1016/j.biortech.2021.126375

Cited by 13 publications

(4 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Solhi et al (2022) demonstrated that the solubilized fraction content of the non-sulfated reaction was approximately fivefold higher than that of the CNC reaction, displaying a much larger difference between the two cellulose substrates than was demonstrated in the current work. Magri et al (2022) used three C1-oxidizing LPMO9s on sulfated CNCs among which only one (MtLPMO9A) produced soluble products. Furthermore, at a higher concentration of CNCs (6.5 wt%), the amount of soluble fraction was amplified, and at this CNC concentration, the other two C1-oxidizing LPMOs (PcLPMO9D and MtLP-MO9D) also produced soluble products.…”

Section: Release Of Soluble Products By Ttlpmo9g On Cncs and Decncsmentioning

confidence: 99%

“…However, proofs of concept for other applications have also emerged. LPMO9s (alone or in combination with cellulases and xylanases) have been demonstrated to produce and oxidize cellulose nanomaterials (Villares et al, 2017;Hu et al, 2018;Moreau et al, 2019;Koskela et al, 2019;Karnaouri et al, 2020;Koskela et al, 2021;Muraleedharan et al, 2021;Rossi et al, 2021;Marjamaa et al, 2022;Magri et al, 2022;Chorozian et al, 2022). In addition, a family AA10 celluloseactive LPMO of bacterial origin has been used to carboxylate cellulose fibers and nanocrystals (Solhi et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Carboxylation of sulfated cellulose nanocrystals by family AA9 lytic polysaccharide monooxygenases

Llàcer Navarro,

Tõlgo,

Olsson

et al. 2023

Cellulose

View full text Add to dashboard Cite

Lytic polysaccharide monooxygenases (LPMOs) from the auxiliary activity 9 (AA9) family act on cellulose through an oxidative mechanism that improves cellulose saccharification in concert with other cellulolytic enzymes. Degradation and solubilization of cellulose chains are known to take place when various cellulose hierarchies, fibers, nanofibers, and cellulose nanocrystals (CNCs) are subjected to LPMOs, either alone or in combination with other cellulose acting enzymes. The use of LPMOs to modify and prepare CNCs has been proposed mostly in top-down synthesis from larger hierarchies. Here, we attempted a direct surface modification of CNCs with LPMOs with the aim of investigating the role played by the charged sulfate groups on CNCs. Sulfate half-ester groups are introduced during the preparation of CNCs from cellulose using sulfuric acid. It has been proposed that the charged sulfate groups hinder the binding of enzymes or affinity of charged reactants on the surface and hence reduce enzymatic and chemical reaction efficiency. We demonstrate the modification of commercial sulfated CNCs using a family AA9 LPMO. Conductometric titration and spectrometric characterization of the oxidized particles indicate that carboxylation of up to 10% was possible without degradation of the crystals. Unexpectedly, the carboxyl groups could only be introduced to the crystals containing sulfate groups, while desulfated crystals remained unfunctionalized. This was deemed to be due to that the sulfate groups limit the adsorption of the enzymes and hence modulate the cuts facilitated by the enzymes on the surface. This limits the release of chains from the surface and enables the carboxylation of the insoluble substrate rather than the release of the solubilized chains. This study highlights the importance of analyzing both the solid and soluble reaction products to gain insights into the oxidation mechanism. We demonstrated that 10% functionalization suffices for the use of CNCs in coupling chemistry.

show abstract

Section: Release Of Soluble Products By Ttlpmo9g On Cncs and Decncsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Carboxylation of sulfated cellulose nanocrystals by family AA9 lytic polysaccharide monooxygenases

Llàcer Navarro,

Tõlgo,

Olsson

et al. 2023

Cellulose

View full text Add to dashboard Cite

show abstract

“…The oxidation of cellulose oligomers (i.e., by LPMO enzymes) releases a blend of oxidized and native COS with different degrees of polymerization (Magri et al, 2022). Previously, we showed that Arabidopsis plants responded differently in the presence of native COS (e.g., cellobiose) or oxiCOS, the latter triggering a wider transcriptional reprogramming (Zarattini, Corso, et al, 2021).…”

Section: Oxicos Confer Thermotolerance In Tandem With Hsfa2mentioning

confidence: 99%

The oxidized cellooligosaccharides confer thermotolerance in Arabidopsis by priming ethylene via heat shock factor A2

Zarattini

Choaibi

Magri

et al. 2022

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

Global climate change, especially heatwaves and aridity, is a major threat to agricultural production and food security. This requires common efforts from the scientific community to find effective solutions to better understand and protect the plant's vulnerabilities to high temperatures. The current study demonstrates the potential of cellooligosaccharides (COS), which are native and oxidized signaling molecules released by lytic polysaccharide monooxygenases (LPMO) enzymes during cell wall degradation by microbial pathogens. The extracellular perception of COS leads to the activation of damage‐triggered immunity, often protecting the plant against biotic stress. However, how these signaling molecules affect abiotic stress tolerance is poorly understood. Here, we show that native COS and oxidized COS (oxiCOS) perception increase the transcript levels of several HEAT SHOCK FACTORS (HSFs) and HEAT SHOCK PROTEINS (HSPs) genes in Arabidopsis plants. However, only oxiCOS treatment triggers ethylene priming and increases thermotolerance. Furthermore, the function of the transcription factor HSFA2 is required for these processes. Altogether, our results indicate that the perception of Damage‐Associated Molecular Patterns (DAMPs) may improve tolerance to adverse abiotic conditions, like exposure to high temperatures.

show abstract

“…Another field that has been exploring LPMOs is nanofibrillated cellulose (NFC) production, Moreau et al (2019) found that treatment of birchwood by LPMO weakened the cellulose fibers cohesion keeping the cellulose crystallinity, showing this step prior to mechanical treatment favored the NFC production. In addition LPMOs can be used for cellulose functionalization (Magri et al, 2021) and, as mentioned above, in plant biomass photobioconversion processes (Blossom et al, 2020). Although, most of LPMOs studies are focused on natural polymers deconstruction, an emerging field of study/application for these enzymes is related to their role in plant pathogenesis and in mutualistic/commensalism symbiosis (Vandhana et al, 2022;Zarattini et al, 2021).…”

Section: Applications and New Perspectivesmentioning

confidence: 99%

Novel Aspergillus fumigatus recombinant LPMOs: biochemical characteristics, and their participation in saccharification and photobiocatalysis processes

Mendoza¹

View full text Add to dashboard Cite

show abstract

Polymer ultrastructure governs AA9 lytic polysaccharide monooxygenases functionalization and deconstruction efficacy on cellulose nano-crystals

Cited by 13 publications

References 46 publications

Carboxylation of sulfated cellulose nanocrystals by family AA9 lytic polysaccharide monooxygenases

Carboxylation of sulfated cellulose nanocrystals by family AA9 lytic polysaccharide monooxygenases

The oxidized cellooligosaccharides confer thermotolerance in Arabidopsis by priming ethylene via heat shock factor A2

Novel Aspergillus fumigatus recombinant LPMOs: biochemical characteristics, and their participation in saccharification and photobiocatalysis processes

Contact Info

Product

Resources

About