Polymerase chain reaction: A landmark in the history of gene technology

Garcia, Joe G.N.; Fan, Shwu

doi:10.1097/01.ccm.0000186782.93865.00

Cited by 15 publications

(10 citation statements)

References 19 publications

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“…Quantification of mRNA coding for specific receptor genes was done using the real-time reverse transcription-polymerase chain reaction (RT-PCR) technique (see review by Garcia et al [15]). All oligonucleotide primers used in this experiment were designed from the published sequence and synthesized by SIGMA-PROLIGO (Sigma-Proligo, Evry, France).…”

Section: Reverse Transcription-real-time Polymerase Chain Reactionmentioning

confidence: 99%

Effects of chronic sepsis on contractile properties of fast twitch muscle in an experimental model of critical illness neuromyopathy in the rat

Rossignol

Guéret

Pennec

et al. 2008

Critical Care Medicine

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Section: Reverse Transcription-real-time Polymerase Chain Reactionmentioning

confidence: 99%

Effects of chronic sepsis on contractile properties of fast twitch muscle in an experimental model of critical illness neuromyopathy in the rat

Rossignol

Guéret

Pennec

et al. 2008

Critical Care Medicine

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“…Quantification of mRNA coding for nAChR subunit genes (ε-and γ-subunits) was done using the real-time RT-PCR technique (see review by Garcia and Ma, 2005). The procedure may be summarized as follows: total RNA was extracted from diaphragms using Trizol reagent (Invitrogen, Cergy Pontoise, France) and RNeasy Mini lit (Qiagen, Valencia, CA, USA) according to manufacturer instructions.…”

Section: Real-time Reverse Transcription Polymerase Chain Reaction (Rmentioning

confidence: 99%

Different magnitude of resistance to non-depolarizing muscle relaxants in dexamethasone-treated rat diaphragm associated with altered acetylcholine receptor expression

Chen¹,

Yang²,

Huang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to investigate the influence of chronic dexamethasone (Dex) administration on rat diaphragm sensitivity to non-depolarizing muscle relaxants (NDMRs) and muscular nicotinic acetylcholine receptor (nAChR) expression, which may help direct future administration of NDMRs. Adult male SpragueDawley rats were randomized to receive a daily intraperitoneal injection of Dex (600 μg/kg body mass) or an equivalent volume of saline (N = 20 in each group) for 14 days. We evaluated isometric twitch tensions of nerve-hemidiaphragm preparations elicited by indirect supramaximal stimulation at 0.1 Hz. Real-time quantitative PCR was performed to determine the mRNA expression of two nAChR subunits (ε-subunit and γ-subunit) in the diaphragm. Dex administration markedly (P < 0.01) increased the 50% twitch depression (IC 50 ) of the three NDMRs. The IC 50 ratio, which standardized the magnitudes of the resistance, was the Differential Dex-induced resistance to muscle relaxants largest for atracurium, with the second largest for vecuronium and the smallest for rocuronium (P < 0.01). The ε-and γ-subunit mRNAs were both upregulated with an increased γ/ε ratio in rats exposed to Dex. The results indicated that chronic Dex administration induces hyposensitivity to NDMRs, the degree of which depends on the kind of neuromuscular blocker, and is associated with increased nAChR expression.

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“…Some of the viral nucleic acids from infected host cells can reach and circulate in the host's peripheral blood prior to cancer development. Polymerase chain reaction (PCR) technology has enabled the detection of human and microbial nucleic acids from samples such as peripheral human blood . This technology has also brought forth the possibility of early detection of oncogenic viruses in peripheral blood, either prior to or at an early stage of cancer development.…”

Section: Introductionmentioning

confidence: 99%

Peripheral Blood Epstein–Barr Viral Nucleic Acid Surveillance as a Marker for Posttransplant Cancer Risk

Dharnidharka

2017

American Journal of Transplantation

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Several viruses, such as Epstein-Barr virus, are now known to be associated with several human cancers, but not all patients with these viral infections develop cancer. In transplantation, such viruses often have a prolonged time gap from infection to cancer development, and many are preceded by a period of circulating and detectable nucleic acids in the peripheral blood compartment. The interpretation of a viral load as a measure of posttransplant risk of developing cancer depends on the virus, the cancer and associated pathogenic factors. This review describes the current state of knowledge regarding the utility and limitations of peripheral blood nucleic acid testing for Epstein-Barr virus in surveillance and risk prediction for posttransplant lymphoproliferative disorders.

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Polymerase chain reaction: A landmark in the history of gene technology

Cited by 15 publications

References 19 publications

Effects of chronic sepsis on contractile properties of fast twitch muscle in an experimental model of critical illness neuromyopathy in the rat

Effects of chronic sepsis on contractile properties of fast twitch muscle in an experimental model of critical illness neuromyopathy in the rat

Different magnitude of resistance to non-depolarizing muscle relaxants in dexamethasone-treated rat diaphragm associated with altered acetylcholine receptor expression

Peripheral Blood Epstein–Barr Viral Nucleic Acid Surveillance as a Marker for Posttransplant Cancer Risk

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