Polymerase chain reaction and restriction fragment length polymorphism of cytocrome oxidase subunit I used for differentiation of Brazilian Biomphalaria species intermediate host of Schistosoma mansoni

Vidigal, Teofânia H. D. A.; Montresor, Lângia C.; Simpson, Andrew J.G.; Carvalho, Omar S.

doi:10.1590/s0074-02762002000900011

Cited by 12 publications

(12 citation statements)

References 25 publications

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Section: Introductionmentioning

confidence: 99%

“…A basic approach to develop such type test has been to employ universal PCR primers to amplify the COI gene and to subject the products to restriction fragment length polymorphism (RFPL) analysis (Vidigal et al 2002;Caldeira et al 2003;Thyssen et al 2005). In addition, a large number of species-specific COI sequences (nearly 150,000 species) have now been submitted to public sequence databases such as GenBank or The Barcode of Life Data System (BOLD) for use in taxonomic and phylogenetic studies of insects (Kwong et al 2012;Ptaszy nska et al 2012;Smith et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Reliable molecular identification of nine tropical whitefly species

Ovalle

Parsa

Hernández

et al. 2014

Ecology and Evolution

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The identification of whitefly species in adult stage is problematic. Morphological differentiation of pupae is one of the better methods for determining identity of species, but it may vary depending on the host plant on which they develop which can lead to misidentifications and erroneous naming of new species. Polymerase chain reaction (PCR) fragment amplified from the mitochondrial cytochrome oxidase I (COI) gene is often used for mitochondrial haplotype identification that can be associated with specific species. Our objective was to compare morphometric traits against DNA barcode sequences to develop and implement a diagnostic molecular kit based on a RFLP-PCR method using the COI gene for the rapid identification of whiteflies. This study will allow for the rapid diagnosis of the diverse community of whiteflies attacking plants of economic interest in Colombia. It also provides access to the COI sequence that can be used to develop predator conservation techniques by establishing which predators have a trophic linkage with the focal whitefly pest species.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Reliable molecular identification of nine tropical whitefly species

Ovalle

Parsa

Hernández

et al. 2014

Ecology and Evolution

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“…PCR-RFLP of the ITS region was used in the identification of species of the genus Lymnaea from different localities in Brazil and Argentina (Carvalho et al, 2004). The COI region was used in the differentiation of three species of Biomphalaria, which are the natural hosts of S. mansoni in Brazil (Vidigal et al, 2002b). The 16S region was employed in the identification of the native species of bivalves Crassostrea brasiliana (Lamarck, 1819) and C. rhizophorae (Guilding, 1828) and the introduced species C. gigas (Thunberg, 1793) (Pie et al, 2006b).…”

Section: Specific Dna Regionsmentioning

confidence: 99%

Molecular studies in Brazilian malacology: Tools, trends and perspectives

2013

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The use of molecular tools in Brazilian malacological studies was analyzed. We searched for these molecular tools by screening the annals of malacological events in Brazil and Latin America within the last 15 years. Information was obtained from a Brazilian curricula data bank (curriculum Lattes), which enabled the identification of the main national research groups, their main researchers and research focuses. An estimate of the scientific production on the basis of the analysis of these curricula is also provided. The main techniques and molecular markers employed were indicated. The data showed that these studies were concentrated in institutions in the South and Southeast regions and emphasized gastropods of medical importance. However, molecular studies that examined other gastropods, cephalopods and bivalves were also performed. No research study was found in the Central-West region of the country. The growth of this field of study in the country is expected, considering the molecular studies that have been performed and the great diversity of the molluscan fauna in Brazil. Future molecular studies on some taxa in the country, which have not yet been studied, should be contemplated. The increase in the use of sequencing techniques will help to bridge the gaps in our taxonomic and phylogenetic knowledge, contributing to the studies on biodiversity and conservation. Keywords: Molecular biology, malacology, snail, Brazil, DNA.

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“…Fixed specimens were identified by means of comparative morphology of the reproductive organs and shells 11 12 13 15 . Total DNA was extracted from the foot of each snail using the Wizard Genomic DNA Purification Kit (Promega) 21 . The ITS2 region was amplified using the primers ITS2F (5'-CGTCCGTCTGAGGGTCGGTTTGC-3) 20 and ETTS1 (5 ' -TGCTTAAGTTCAGCGGGT-3') 7 anchored in the conserved extremities of the 5.8S and 28S ribosomal genes, respectively.…”

mentioning

confidence: 99%

Polymerase chain reaction and restriction fragment length polymorphism analysis of the ITS2 region for differatiation of Brazilian Biomphalaria intermediate hosts of the Schistosoma mansoni

Vidigal

Magalhães

Carvalho

2004

Rev. Soc. Bras. Med. Trop.

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We sequenced the internal transcribed spacer 2 of the ribosomal DNA (ITS2-DNAr) from the three Schistosoma mansoni intermediate hosts in Brazil: Biomphalaria glabrata, Biomphalaria tenagophila and Biomphalaria straminea. Analysis of a restriction map from those sequences allowed us to select putative restriction enzymes able to identify the snail species under study. Four restriction enzymes were used and HpaII provided simple species-specific profiles easily visualized in polyacrylamide gels. The use of ITS2 is advantageous as it provides a small fragment of 460 bp which may be easily amplified by PCR. In the current work, we showed that the amplification of ITS2-DNAr together with HpaII enzyme restriction is an auxiliary molecular tool for the morphological identification of such snails as well as for taxonomic and phylogenetic studies of neotropical planorbids.

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Polymerase chain reaction and restriction fragment length polymorphism of cytocrome oxidase subunit I used for differentiation of Brazilian Biomphalaria species intermediate host of Schistosoma mansoni

Cited by 12 publications

References 25 publications

Reliable molecular identification of nine tropical whitefly species

Reliable molecular identification of nine tropical whitefly species

Molecular studies in Brazilian malacology: Tools, trends and perspectives

Polymerase chain reaction and restriction fragment length polymorphism analysis of the ITS2 region for differatiation of Brazilian Biomphalaria intermediate hosts of the Schistosoma mansoni

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