2013
DOI: 10.17660/actahortic.2013.973.27
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Polymerase Chain Reaction Based Detection of Chilli Anthracnose Disease

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“…COL1/COL2 primers were used for amplification of the specific internal transcribed spacer region of tested Colletotrichum species (C. acutatum, C. truncatum and C. gloeosporioides) with a specific band of 460 base pairs. C. gloeosporioides was detected at a low level of 1000 conidia on chilli leaf and fruit by this primer [62]. Another, primer set based on the sequences of the ribosomal internal transcribed spacer (ITS1and ITS2) regions of C. truncatum was designed and standardized for the detection of C. truncatum in infected plant tissues using PCR assay.…”
Section: Diagnosismentioning
confidence: 99%
“…COL1/COL2 primers were used for amplification of the specific internal transcribed spacer region of tested Colletotrichum species (C. acutatum, C. truncatum and C. gloeosporioides) with a specific band of 460 base pairs. C. gloeosporioides was detected at a low level of 1000 conidia on chilli leaf and fruit by this primer [62]. Another, primer set based on the sequences of the ribosomal internal transcribed spacer (ITS1and ITS2) regions of C. truncatum was designed and standardized for the detection of C. truncatum in infected plant tissues using PCR assay.…”
Section: Diagnosismentioning
confidence: 99%