Polymerase chain reaction based epidemiological investigation of canine parvoviral disease in dogs at Bareilly region

Thomas, Jobin; Singh, Mithilesh; Goswami, T. K.; Verma, Suman; Badasara, Surendra Kumar

doi:10.14202/vetworld.2014.929-932

Cited by 12 publications

(13 citation statements)

References 11 publications

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“…Of 260 positive cases, 94 were females (36.15%), and 133 (51.15%) were males. Coincidently, the current finding is consistent with studies by Thomas et al [38] and Gombac et al [39], as well as other reliable publications [34,38,[40][41][42]. Many assumptions could be analyzed; however, the most relevant cause could be that the majority of samples were collections from male dogs during the study.…”

Section: Discussionsupporting

confidence: 93%

Molecular epidemiology of canine parvovirus type 2 in Vietnam from November 2016 to February 2018

Hoang

Lin

et al. 2019

Virol J

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Background Canine parvovirus type 2 (CPV-2) was first identified in the late 1970s; it causes intestinal hemorrhage with severe bloody diarrhea in kennels and dog shelters worldwide. Since its emergence, CPV-2 has been replaced with new genetic variants (CPV-2a, CPV-2b, and CPV-2c). Currently, information about the genotype prevalence of CPV-2 in Vietnam is limited. In the present study, we investigated the genotype prevalence and distribution of CPV-2 in the three regions of Vietnam. Methods Rectal swabs were collected from 260 dogs with suspected CPV-2 infection from northern, central, and southern Vietnam from November 2016 to February 2018. All samples were identified as parvovirus positive by real-time PCR, and further genotyping was performed using a SimpleProbe® real-time PCR assay. Results Of the 260 Vietnamese CPV-2 isolates, 6 isolates (2.31%) were identified as CPV-2a, 251 isolates (96.54%) were identified as CPV-2c and 3 isolates (1.15%) were untypable using the SimpleProbe® real-time PCR assay. In northern Vietnam, the percentages of CPV-2a and CPV-2c were 2.97% (3/101) and 97.3% (98/101), respectively. In central Vietnam, the percentages of CPV-2a and CPV-2c were 1.11% (1/90) and 98.89% (89/90), respectively. In southern Vietnam, the percentages of CPV-2a and CPV-2c were 3.03% (2/66) and 96.97% (64/66), respectively. CPV-2b was not observed in this study. The VP2 genes of CPV-2c in Vietnam are more genetically similar to those of CPV-2c strains in China and Taiwan than to those of prototype CPV-2c strains (FJ222821) or the first Vietnamese CPV-2c (AB120727). Conclusion The present study provides evidence that CPV-2c is the most prevalent variant in Vietnam. Phylogenetic analysis demonstrated that the recent Vietnamese CPV-2c isolates share a common evolutionary origin with Asian CPV-2c strains. Electronic supplementary material The online version of this article (10.1186/s12985-019-1159-z) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 93%

Molecular epidemiology of canine parvovirus type 2 in Vietnam from November 2016 to February 2018

Hoang

Lin

et al. 2019

Virol J

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“…On the age-wise incidence of CPV were analyzed and the higher positivity was encountered in both less than 3 months (78.08 %) and 3 to 6 months of age groups (77.42%) and 34.50% of incidence in 6 to 12 months of age group (Table 1). The increased susceptibility of less than 6 months of age dogs for CPE were in agreement with other researchers (Umar et al, 2015;Thomas et al, 2014;Strepita et al, 2013) who documented the increased chance of CPV in less than 3 months followed by 3 -6 months and 6 -12 months of age group. Due to the affinity of CPV to the mitotic intestinal cells and gut flora and fauna changes especially during weaning time may facilitate the increased chances of CPV in less than 3 months of age group.…”

Section: Resultssupporting

confidence: 92%

Factors Associated with the Occurrence of Canine Parvoviral Enteritis in Dogs

Naveenkumar¹

2019

JAR

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A prospective study was conducted to identify the risk factors associated with the incidence of Canine Parvoviral Enteritis (CPE) in dogs. Total of 120 animals screened using PCR assay, 72.50 percentage of animal were found positive for Canine Parvo Virus (CPV). Incidence in history of unvaccinated and vaccinated dogs was 79.69 and 64.29 per cent respectively. Age-wise predisposition of CPE indicated that the highest incidence was observed in both less than 3 months (78.08 %) and 3 to 6 months of age group (77.42 %) followed by 6 to 12 months of age group (34.50 %). Incidence of CPE in scheduled and unscheduled vaccination was 30.00 and 83.33 per cent respectively. In this study, unvaccinated status, unscheduled vaccination and young age groups are found to be significant risk factors associated with the occurrence of CPE.

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“…Thomas et al . [ 24 ] also reported a higher incidence of CPV (78.26%) in male dogs. Reason behind this high incidence in males may be because most of the admitted dogs (70.42%) were male [ 24 ].…”

Section: Discussionmentioning

confidence: 98%

“…[ 24 ] also reported a higher incidence of CPV (78.26%) in male dogs. Reason behind this high incidence in males may be because most of the admitted dogs (70.42%) were male [ 24 ]. However again it can be explained that as male dogs are mostly suffering from an infection, for which more male dogs were being admitted for treatment.…”

Section: Discussionmentioning

confidence: 99%

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Epidemiological study of canine parvovirus infection in and around Bhubaneswar, Odisha, India

Behera¹,

Panda²,

Sahoo³

et al. 2015

Vet World

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Aim:An epidemiological study of canine parvovirus infection in dogs in and around Bhubaneswar, Odisha was conducted between December 2012 to March 2013 and prevalence rate was studied on the basis of age, breed, and sex.Materials and Methods:A total of 71 fecal samples from suspected diarrheic dogs were collected in sterile phosphate buffer saline (10% W/V) and examined by polymerase chain reaction (PCR) for detection of canine parvo virus infection, followed by epidemiological study in relation to age, breed, and sex.Results:Of 71 samples analyzed, 29 (40.85%) were found to be positive by PCR assay. The infection was higher in Deshi/local breeds (34.48%), followed by German shepherd (17.24%), equal incidences in mixed and Labrador retriever (10.34%), Rottweiler and German spitz showed 6.90% each and finally lower incidences in four breeds (3.45%) such as Dalmatians, Nea politan mastiff, Pug and Great Dane. Age-wise prevalence study revealed the infection being more in the age group of 3-6 months (41.37%), followed by equal incidences of 27.59% in 1-3 months and 6-12 months age group, and a low incidence in age groups above 12 months (3.45%). The incidence was predominantly higher in males (86.21%) than females (13.79%).Conclusions:The epidemiological analysis revealed that the breed wise prevalence was found to be more in Deshi breeds as compared to others, age groups below 6 months were found to be more prone to parvovirus infection and males were mostly infected.

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Polymerase chain reaction based epidemiological investigation of canine parvoviral disease in dogs at Bareilly region

Cited by 12 publications

References 11 publications

Molecular epidemiology of canine parvovirus type 2 in Vietnam from November 2016 to February 2018

Molecular epidemiology of canine parvovirus type 2 in Vietnam from November 2016 to February 2018

Factors Associated with the Occurrence of Canine Parvoviral Enteritis in Dogs

Epidemiological study of canine parvovirus infection in and around Bhubaneswar, Odisha, India

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