Polymerase chain reaction based human leucocyte antigen genotyping for the investigation of suspected gastrointestinal biopsy contamination

Abstract

Background-Mislabelling or contamination of surgical specimens may lead to diagnostic inaccuracy, particularly within gastrointestinal pathology when multiple small mucosal biopsy specimens are commonly taken, and where a tiny fragment of foreign tissue may be indistinguishable from true biopsy material using histological assessment alone. Conclusions-PCR based HLA class II genotyping is a valuable tool for investigating potential contamination or mislabelling within gastrointestinal biopsy specimens and this report has confirmed contamination in seven of ten cases studied. Aims-To

Multiplex DNA Short Tandem Repeat Analysis

“…tissues or cells in surgical pathology or cytology case material. 2,4,[7][8][9][10][11] The use of the STR multiplex system described herein is the method of choice for forensic identity testing because of its greater sensitivity and higher discriminating power than the methods reported previously in the pathology literature. 12 Its applicability to detection and determination of the origin of minute contaminants in surgical pathology was demonstrated in the present study.…”

Contamination of histology biopsy specimen - a potential source of error for surgeons: a case report

“…Tissue specimen mix ups or 'carry-over' are a challenging problem in surgical pathology practice [ 1 ]. Surgeons should be aware that this a recurring problem in practice [ 2 ], and similar cases have occurred [ 3 ]-[ 5 ]. The reported rates of occurrence of contaminant tissues or cells have ranged from 0 to 8.8% (including prospective and retrospective cases) [ 6 ].…”