Polymerase chain reaction Confirmation of<i>Babesia canis canis</i>and<i>Anaplasma phagocytophilum</i>in Dogs Suspected of Babesiosis in Slovakia

Majláthová, Viktória; Majláth, Igor; Víchová, Bronislava; Gul’ová, Ivana; Derdáková, Markéta; Sesztáková, E.; Peťko, Branislav

doi:10.1089/vbz.2010.0276

Cited by 29 publications

(16 citation statements)

References 38 publications

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“…M'Ghirbi & Bouattour (2008) obtained a prevalence of 0.6% in Tunisia. Higher prevalence was found in 35.6% ticks in Slovakia (Majláthová et al 2011).…”

Section: Discussionmentioning

confidence: 85%

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Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil

et al. 2015

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Abstract:This study aimed to report the prevalence of Babesia canis vogeli in dogs and ticks in the urban and rural areas of Petrolina, Pernambuco. Serum and peripheral blood samples of 404 dogs were tested by indirect immunofluorescence assay (IFA) and by blood smears, respectively. The presence of tick infestation was evaluated, and some specimens were submitted to DNA amplification by polymerase chain reaction (PCR). The presence of antibodies anti-B. canis vogeli was determinate in 57.9% (234/404) of dogs. The direct detection of Babesia spp was obtained in 0.5% (2/404) dogs by visualization of intraerythrocytic forms. Infestation by Rhipicephalus sanguineus sensu lato was observed in 54.5% (220/404) of dogs in both urban and rural areas. DNA of Babesia canis vogeli were obtained by PCR in 6% individual (3/50) and 8.7% of pool of ticks (7/80). The risk factors for the presence of anti-B. canis vogeli antibodies, as determined through the application of logistic regression models (P<0.05), were the following: medium breed size variables (P<0.001); contact with areas of forest (P=0.021); and access on the street (P=0.046). This study describes, for the first time, the confirmation of infection of B. canis vogeli in dogs and ticks in the semiarid region of Pernambuco, Brazil.

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“…M'Ghirbi & Bouattour (2008) obtained a prevalence of 0.6% in Tunisia. Higher prevalence was found in 35.6% ticks in Slovakia (Majláthová et al 2011).…”

Section: Discussionmentioning

confidence: 85%

“…Detection of Babesia infection in ticks has been widely used in epidemiological studies in various countries , Matjila et al 2005, M'Ghirbi & Bouattour 2008, Majláthová et al 2011. However, in Brazil, few studies have been performed and little is known about the aspects of infection with Babesia spp.…”

Section: Discussionmentioning

confidence: 99%

Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil

et al. 2015

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“…In BLAST Majlathova et al 2011;Kubelova et al 2013). B. canis was previously reported from the CR; however, all cases were in dogs with a history of travel to endemic areas (Kucera 1992;Svobodova and Svobodova 2004).…”

Section: Resultsmentioning

confidence: 99%

“…Based on this, one can assume that the spread of B. canis follows the natural distribution of its vector. However, the data on B. canis infection in dogs or ticks in areas of Slovakia and Austria adjacent to the borders of the Czech Republic are either absent, or infection was Majlathova et al 2011;Kubelova et al 2013;Halos et al 2014;Pantchev et al 2015). Recent phylogenetic studies suggested intraspecies diversity of B. canis.…”

Section: Resultsmentioning

confidence: 99%

Autochthonous Babesia canis, Hepatozoon canis and imported Babesia gibsoni infection in dogs in the Czech Republic

Mitková¹,

Hrazdilová²,

Novotná³

et al. 2017

Vet. Med.

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ABSTRACT:The epidemiology of tick-borne diseases as well as the ecology of the associated tick vectors is currently undergoing dynamic change. Canine babesiosis has, to date, only been reported in the Czech Republic as an imported disease, despite the presence of an established population of D. reticulatus in the South Moravian region. A recent report of autochthonous H. canis in dogs indicates that the spectrum of tick-borne diseases in dogs in the Czech Republic may be broader than previously expected and that a survey of a large set of dogs may be required. The aim of the present study was to survey the spectrum of tick-borne apicomplexan parasites infecting dogs in the Czech Republic and to assess the autochthonous character of detected infections. In the present study, we surveyed the spectrum of tick-borne protists in 418 dogs in the Czech Republic using PCR-based detection of the 18S rDNA gene of piroplasms and H. canis. We confirmed the presence of H. canis (4%), B. canis (1%, including the autochthonous cases) and a single imported case of B. gibsoni (0.2%) infection in sampled animals. Our results indicate that these pathogens deserve closer attention from veterinarian practitioners.

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“…Canine babesiosis caused by B. canis is considered an emerging disease spreading with its vector Dermacentor reticulatus (Fabricius) (Zygner et al 2009). Clinical cases of babesiosis caused by B. canis are milder and have been reported from countries neighbouring with the Czech Republic, such as Austria, Germany, Poland and Slovakia (Zahler et al 2000, Chandoga et al 2002, Adaszek and Winiarczyk 2008, Majláthová et al 2011, Silaghi et al 2011). The more pathogenic B. gibsoni has been recently reported from Slovakia (Víchová et al 2016).…”

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confidence: 99%

Variability of species of Babesia Starcovici, 1893 in three sympatric ticks (Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna) at the edge of Pannonia in the Czech Republic and Slovakia

et al. 2017

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Abstract:The distribution, variability and host specificity of species of Babesia Starcovici, 1893 were studied in questing ticks collected on the northwestern edge of the Pannonian Basin in the south-easternmost part of the Czech Republic and in western Slovakia. The area is characterised by relatively natural floodplain habitats and the sympatric occurrence of three tick species possessing wide host spectra, namely Ixodes ricinus (Linnaeus), Dermacentor reticulatus (Fabricius) and Haemaphysalis concinna Koch. Analysis was carried out on 1,408 I. ricinus, 2,999 D. reticulatus and 150 H. concinna altogether, collected from 59 localities. We documented the presence of Babesia spp. not only in I. ricinus but also in H. concinna in the Czech Republic. Two isolates from I. ricinus were classified as B. venatorum Herwaldt, Cacciò, Gherlinzoni, Aspöck, Slemenda, Piccaluga, Martinelli, Edelhofer, Hollenstein, Poletti, Pampiglione, Löschenberger, Tura et Pieniazek, 2003 (formerly determined as Babesia sp. EU1), which is a zoonotic parasite and can cause human babesiosis. The rest of our amplicons were very similar to B. canis (Piana et Galli-Valerio, 1895), which is usually transmitted by D. reticulatus. Despite the huge amount of examined samples, all D. reticulatus ticks were Babesia-free. Due to this finding, we did not consider our obtained isolates to be B. canis, but other closely related species possessing a similar sequence of the studied portion of 18S rDNA. Although this genetic marker is most frequently used in PCR-based diagnostic methods of babesias, its low variability compromises its reliability in studies based only on this marker.

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Polymerase chain reaction Confirmation ofBabesia canis canisandAnaplasma phagocytophilumin Dogs Suspected of Babesiosis in Slovakia

Cited by 29 publications

References 38 publications

Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil

Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil

Autochthonous Babesia canis, Hepatozoon canis and imported Babesia gibsoni infection in dogs in the Czech Republic

Variability of species of Babesia Starcovici, 1893 in three sympatric ticks (Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna) at the edge of Pannonia in the Czech Republic and Slovakia

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