Polymerase chain reaction for the diagnosis of herpesvirus infections in dermatology

Schremser, Verena; Antoniewicz, Lukasz; Tschachler, Erwin; Geusau, Alexandra

doi:10.1007/s00508-019-01585-w

Cited by 7 publications

(2 citation statements)

References 20 publications

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“…A fluorescence dye (SYBR green) or TaqMan probe is used in real-time PCR to monitor DNA amplification as the reaction progresses. Over the years, different PCR assays have been used for the detection of HSV [ 97 , 100 , 101 , 102 , 103 , 104 , 105 ]. Marshall et al have detected HSV from genital infections using a multiplex PCR assay [ 106 ].…”

Section: Detection Of Hsv In Lesionsmentioning

confidence: 99%

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Nath

Kabir

Doust

et al. 2021

Infectious Disease Reports

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Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages.

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Section: Detection Of Hsv In Lesionsmentioning

confidence: 99%

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Nath

Kabir

Doust

et al. 2021

Infectious Disease Reports

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“…Nail infection may be investigated by testing the pustule material (e.g. gram stain for possible bacterial infection; potassium hydroxide test for possible fungal infection; polymerase chain reaction testing for possible herpetic whitlow [41]).…”

Section: What Are the Differential Diagnoses?mentioning

confidence: 99%

A brief guide to pustular psoriasis for primary care providers

Crowley¹,

Pariser

Yamauchi

2020

Postgraduate Medicine

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Pustular psoriasis refers to a heterogeneous group of chronic inflammatory skin disorders that are clinically, histologically, and genetically distinct from plaque psoriasis. Pustular psoriasis may present as a recurrent systemic illness (generalized pustular psoriasis [GPP]), or as localized disease affecting the palms and/or soles (palmoplantar pustulosis [PPP], also known as palmoplantar pustular psoriasis), or the digits/nail beds (acrodermatitis continua of Hallopeau [ACH]). These conditions are rare, but their possible severity and consequences should not be underestimated. GPP, especially an acute episode (flare), may be a medical emergency, with potentially life-threatening complications. PPP and ACH are often debilitating conditions. PPP is associated with impaired health-related quality of life and psychiatric morbidity, while ACH threatens irreversible nail and/or bone damage. These conditions can be difficult to diagnose; thus, primary care providers should not hesitate to contact a dermatologist for advice and/or patient referral. The role of corticosteroids in triggering and leading to flares of GPP should also be noted, and physicians should avoid the use of systemic corticosteroids in the management of any form of psoriasis.

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Human Herpesviruses: Varicella and Herpes Zoster

Gnann

2023

Viral Infections of Humans

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Polymerase chain reaction for the diagnosis of herpesvirus infections in dermatology

Cited by 7 publications

References 20 publications

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

A brief guide to pustular psoriasis for primary care providers

Human Herpesviruses: Varicella and Herpes Zoster

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