Polymerase chain reaction to detect human cytomegalovirus in livers of infants with neonatal hepatitis

Chang, Mei–Hwei; Huang, Han-Ching; Huang, Eng‐Shang; Kao, Chuan-Liang; Hsu, Hong–Yuan; Lee, Chin‐Yun

doi:10.1016/0016-5085(92)90038-z

Cited by 79 publications

(57 citation statements)

References 9 publications

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“…In the literature, the frequency of positive CMV in NIHC by PCR techniques varies between 5% and 46% [14,42,43] . Different techniques and samples make it difficult to establish a correlation between the data.…”

Section: Discussionmentioning

confidence: 99%

“…A retrospective analysis of 39 patients (who presented with CMV in blood or urine cultures, or positive PCR in liver biopsies collected between 30 and 220 d of life) showed the presence of CMV by PCR-CMV on filter papers collected during the first three days of life in two neonates, suggesting that congenital infection was not the cause of cholestasis [42] . Chang et al [14] studied 50 children diagnosed with neonatal hepatitis by PCR-CMV of liver samples Twenty-three children had positive PCR, 13 of them had serology suggestive of an acute infection, and nine children had negative serologies; in one case serology was unknown. Twenty-one of the 27 patients with a negative PCR presented with negative serologies and six had positive serologies.…”

Section: Discussionmentioning

confidence: 99%

“…The most common causes of the disease are infection, including cytomegalovirus (CMV) [10][11][12] . Data on the prevalence of CMV in NIHC vary greatly depending on the diagnostic method used (5%-46%) [2,[12][13][14] . Congenital CMV infection is assessed by viral isolation detected within 2-3 wk after birth [15] .…”

Section: Introductionmentioning

confidence: 99%

“…Congenital CMV infection is assessed by viral isolation detected within 2-3 wk after birth [15] . If CMV is detected three weeks after birth, diagnosis of congenital infection should be supported by clinical and epidemiologic features [12,14,16] . Congenital disease might occur due to maternal primary infection (where the vertical transmission rate ranges from 40%-50%), or as a recurrence (where the vertical transmission rate ranges from 0.5%-2%).…”

Section: Introductionmentioning

confidence: 99%

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Cytomegalovirus frequency in neonatal intrahepatic cholestasis determined by serology, histology, immunohistochemistry and PCR

Bellomo-Brandão¹,

Andrade²,

Costa³

et al. 2009

WJG

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AIM:To determine cytomegalovirus (CMV) frequency in neonatal intrahepatic cholestasis by serology, histological revision (searching for cytomegalic cells), immunohistochemistry, and polymerase chain reaction (PCR), and to verify the relationships among these methods. METHODS:The study comprised 101 non-consecutive infants submitted for hepatic biopsy between March 1982 and December 2005. Serological results were obtained from the patient's files and the other methods were performed on paraffin-embedded liver samples from hepatic biopsies. The following statistical measures were calculated: frequency, sensibility, specific positive predictive value, negative predictive value, and accuracy. RESULTS:T he fre que nc ie s o f po s i t ive re s u l t s were as follows: serology, 7/64 (11%); histological revision, 0/84; immunohistochemistry, 1/44 (2%), and PCR, 6/77 (8%). Only one patient had positive immunohistochemical findings and a positive PCR. The following statistical measures were calculated between PCR and serology: sensitivity, 33.3%; specificity, 88.89%; positive predictive value, 28.57%; negative predictive value, 90.91%; and accuracy, 82.35%. CONCLUSION:The frequency of positive CMV varied among the tests. Serology presented the highest positive frequency. When compared to PCR, the sensitivity and positive predictive value of serology were low.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cytomegalovirus frequency in neonatal intrahepatic cholestasis determined by serology, histology, immunohistochemistry and PCR

Bellomo-Brandão¹,

Andrade²,

Costa³

et al. 2009

WJG

View full text Add to dashboard Cite

show abstract

“…CMV infection is one of the main causes of neonatal and infantile hepatitis (5)(6)(7)23), but confirmation of the association of CMV infection with hepatitis is sometimes difficult. Primary CMV infection is typically determined by the detection of serum anti-CMV IgM antibody or the seroconversion of IgG antibody.…”

Section: Discussionmentioning

confidence: 99%

Association of Cytomegalovirus with Infantile Hepatitis

Shibata

Kitajima

Kawada

et al. 2005

Microbiology and Immunology

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Infantile hepatitis is occasionally seen in apparently healthy children. In most cases, the etiology of the infection is uncertain. However, cytomegalovirus (CMV), Epstein‐Barr virus (EBV), human herpesvirus‐6 (HHV‐6), human herpesvirus‐7 (HHV‐7), human parvovirus B19, and TT virus (TTV) are considered to be associated with hepatitis in children. The objective of this study was to investigate the correlations between these viruses and infantile hepatitis. Twenty‐six children from 1 to 24 months old (median age, 7 months) who had liver dysfunction of unknown etiology were enrolled in this study. Plasma samples were examined by a real‐time PCR assay for CMV, EBV, HHV‐6, HHV‐7, parvovirus B19, and TTV DNA. The DNA of CMV was detected in the plasma of four patients (15.4%) and was detected significantly more often in the patient group than in the control group. The CMV‐infected patients were 1 to 3 months old, which was significantly younger than the remaining patients. The serological findings did not always correlate with the results of the real‐time PCR assay. The DNA of TTV was detected in four patients (15.4%), while human parvovirus B19 DNA was detected in three (11.5%). However, the detection frequencies of these viral DNAs were not significantly different from those in the control groups, and some of these patients had co‐infections. These results indicate that CMV might be one of the major pathogens responsible for infantile hepatitis; however, serological tests have limited utility for the diagnosis of CMV infection in young children.

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The Liver in Infancy and Childhood

Sherlock¹,

Dooley²

2001

Diseases of the Liver and Biliary System

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Polymerase chain reaction to detect human cytomegalovirus in livers of infants with neonatal hepatitis

Cited by 79 publications

References 9 publications

Cytomegalovirus frequency in neonatal intrahepatic cholestasis determined by serology, histology, immunohistochemistry and PCR

Cytomegalovirus frequency in neonatal intrahepatic cholestasis determined by serology, histology, immunohistochemistry and PCR

Association of Cytomegalovirus with Infantile Hepatitis

The Liver in Infancy and Childhood

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