Polymeric materials for bone and cartilage repair

Puppi, Dario; Chiellini, Federica; Piras, Anna Maria; Chiellini, Emo

doi:10.1016/j.progpolymsci.2010.01.006

Cited by 823 publications

(594 citation statements)

References 478 publications

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“…A scaffold should possess an interconnected and uniformly distributed porosity with a highly porous surface and microstructure [31]. According to previous reported results, a pore size between 200 and 400 lm is suitable for cell adhesion and ingrowth in vitro and neovascularization in vivo [4]. When the pores are smaller than this, pore occlusion by the cells could occur, inhibiting ECM production [32].…”

Section: Thermal Analysismentioning

confidence: 98%

“…Allograft bone also has concerns related to disease transmission risk and infection, explaining why synthetic bone grafts are increasingly being employed in the clinical field [1]. Certain biomaterials can repair or replace damaged or diseased tissue by mimicking the natural extracellular matrix (ECM) [4]. Bio-resorbable scaffolds degrade in situ and minimize the need for additional surgery to remove the implant [5].…”

Section: Introductionmentioning

confidence: 99%

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Fabrication and characterization of poly(octanediol citrate)/gallium-containing bioglass microcomposite scaffolds

et al. 2014

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Bone can be affected by osteosarcomae requiring surgical excision of the tumor as part of the treatment regime. Complete removal of cancerous cells is difficult and conventionally requires the removal of a margin of safety around the tumor to offer improved patient prognosis. This work considers a novel series of composite scaffolds based on poly(octanediol citrate) (POC) impregnated with galliumbased bioglass microparticles for possible incorporation into bone following tumor removal. The objective of this research was to fabricate and characterize these scaffolds and subsequently report on their mechanical and biological properties. The porous microcomposite scaffolds with various concentrations of bioglass (10, 20, 30 wt%) incorporated were fabricated using a salt leaching technique. The scaffolds exhibited compression modulus in the range of 0.3-7 MPa. The addition of bioglass increased the mechanical properties even though porosity increased. Furthermore, increasing the concentration of bioglass had a significant influence on glass transition temperature from 2.5°C for the pure polymer to around 25°C for 30 % bioglass-containing composite. The ion release study revealed that composites containing 10 % bioglass had the highest ion release ratio after 28 days of soaking in phosphate buffered saline. The interaction of bioglass phase with POC led to the formation of additional ionic crosslinks aside from covalent crosslinks which further resulted in increased stiffness and decreased weight loss. The osteoblast cells were well attached and growth on composites and collagen synthesis increased particularly with the 10 % bioglass concentration.

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Section: Thermal Analysismentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of poly(octanediol citrate)/gallium-containing bioglass microcomposite scaffolds

et al. 2014

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“…n the field of bone tissue engineering, numerous scaffold types, such as ceramic scaffolds, (1)(2)(3) polymeric scaffolds, (4,5) and composite scaffolds, (6)(7)(8) have been tested in vivo. These osteoconductive scaffolds are often combined with growth factors (osteoinductive) (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) and/or with cells (osteogenic) (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32) to induce active stimulation of bone regeneration.…”

mentioning

confidence: 99%

Porous scaffold architecture guides tissue formation

Cipitria

Lange

Schell

et al. 2012

Journal of Bone and Mineral Research

104

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Critical-sized bone defect regeneration is a remaining clinical concern. Numerous scaffold-based strategies are currently being investigated to enable in vivo bone defect healing. However, a deeper understanding of how a scaffold influences the tissue formation process and how this compares to endogenous bone formation or to regular fracture healing is missing. It is hypothesized that the porous scaffold architecture can serve as a guiding substrate to enable the formation of a structured fibrous network as a prerequirement for later bone formation. An ovine, tibial, 30-mm critical-sized defect is used as a model system to better understand the effect of the scaffold architecture on cell organization, fibrous tissue, and mineralized tissue formation mechanisms in vivo. Tissue regeneration patterns within two geometrically distinct macroscopic regions of a specific scaffold design, the scaffold wall and the endosteal cavity, are compared with tissue formation in an empty defect (negative control) and with cortical bone (positive control). Histology, backscattered electron imaging, scanning small-angle X-ray scattering, and nanoindentation are used to assess the morphology of fibrous and mineralized tissue, to measure the average mineral particle thickness and the degree of alignment, and to map the local elastic indentation modulus. The scaffold proves to function as a guiding substrate to the tissue formation process. It enables the arrangement of a structured fibrous tissue across the entire defect, which acts as a secondary supporting network for cells. Mineralization can then initiate along the fibrous network, resulting in bone ingrowth into a critical-sized defect, although not in complete bridging of the defect. The fibrous network morphology, which in turn is guided by the scaffold architecture, influences the microstructure of the newly formed bone. These results allow a deeper understanding of the mode of mineral tissue formation and the way this is influenced by the scaffold architecture. ß

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“…To overcome these obstacles, bone tissue engineering using scaffolds and stem cells have become an attractive subject of research in the past few years. Engineering of these cellular constructs may form a suitable alternative in bone regeneration therapies (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

Osteogenic Differentiation and Mineralization on Compact Multilayer nHA-PCL Electrospun Scaffolds in a Perfusion Bioreactor

et al. 2016

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Background:Monolayer electrospun scaffolds have already been used in bone tissue engineering due to their high surface-tovolume ratio, interconnectivity, similarity to natural bone extracellular matrix (ECM), and simple production. Objectives: The aim of this study was to evaluate the dynamic culture effect on osteogenic differentiation and mineralizationi into a compact cellular multilayer nHA-PCL electrospun construct. The dynamic culture was compared with static culture. Materials and Methods:The calcium content, alkaline phosphatase (ALP) activity and cell viability were investigated on days 3 and 7. Results: When the dynamic culture compared to static culture, the mineralization and ALP activity were increased in dynamic culture. After 7 days, calcium contents were 41.24 and 20.44 μg.(cm 3 ) -1 , and also normalized ALP activity were 0.32 and 0.19 U.mg -1 in dynamic and static culture, respectively. Despite decreasing the cell viability until day 7, the scanning electron microscopy (SEM) results showed that, due to higher mineralization, a larger area of the construct was covered with calcium deposition in dynamic culture. Conclusions:The dynamic flow could improve ALP activity and mineralization into the compact cellular multilayer construct cultured in the perfusion bioreactor after 7 days. Fluid flow of media helped to facilitate the nutrients transportation into the construct and created uniform cellular construct with high mineralization. This construct can be applied for bone tissue engineering.

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Polymeric materials for bone and cartilage repair

Cited by 823 publications

References 478 publications

Fabrication and characterization of poly(octanediol citrate)/gallium-containing bioglass microcomposite scaffolds

Fabrication and characterization of poly(octanediol citrate)/gallium-containing bioglass microcomposite scaffolds

Porous scaffold architecture guides tissue formation

Osteogenic Differentiation and Mineralization on Compact Multilayer nHA-PCL Electrospun Scaffolds in a Perfusion Bioreactor

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