BackgroundRoot‐knot nematodes (RKN) are the highly damaging pests for various crops, and the prevalence of RKN has posed serious threats to worldwide agricultural harvest, severely affecting global food security and ecosystem health. Traditional pesticide systems on controlling RKN generally cause environmental hazards and phytotoxicity due to the excessive use of pesticides resulted from low utilization efficiency. And effective approaches with biosafe and efficient features are highly demanded to break away from the dilemma caused by RKN.ResultsIn this research, a nanopesticide system with root‐targeted delivery function was developed to achieve effective prevention and control of RKN. The nanocarriers (MSN‐KH560‐Gly) and the obtained nanopesticides (EB@MSN‐KH560‐Gly) were proved to be biosafe. Also, this nanopesticide system demonstrated sustained release behavior. The grafting of glycine (Gly) significantly improved the pesticide contents translocating to cucumber roots (about 304.7%). Besides, such root‐targeted delivery function resulted in no root nodule in cucumber plants after the foliar application of these nanopesticides (prevention rate of 100%). In addition, the root nodule numbers of the infected cucumber plants decreased by 71.67%.ConclusionFoliar application of these glycine‐functionalized nanopesticides achieved effective prevention and control of RKN due to the root‐targeted delivery property inherent in this nanopesticide system, and such root‐targeted delivery strategy opens a novel and efficient method to protect crops from RKN invasion and thus facilitates the development of sustainable agriculture.This article is protected by copyright. All rights reserved.