Polymeric nanoparticles as oral delivery systems for a grape pomace extract towards the improvement of biological activities

Costa, Joana R.; Martín, Xavier; Amado, Isabel Rodríguez; Gonçalves, Catarina; Tonon, Renata V.; Cabral, Lourdes Maria Corrêa; Pastrana, Lorenzo; Pintado, Manuela

doi:10.1016/j.msec.2020.111551

Cited by 30 publications

(16 citation statements)

References 48 publications

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“…± 126.96 µmol TE/g d.w.) [ 47 ]. Chitosan-encapsulated grape pomace nanoparticles increased their ORAC from 2058 to 2463 μmol TE/g after digestion [ 50 ]. Most polyphenols are degraded along the digestive tract due to pH and digestive enzymes [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

Anthocyanins Found in Pinot Noir Waste Induce Target Genes Related to the Nrf2 Signalling in Endothelial Cells

et al. 2022

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Grape pomace is a source of anthocyanins, which can prevent cardiovascular diseases due to their antioxidant properties. Anthocyanin activity is associated with the ability to regulate oxidative stress through the transcription factor Nrf2. Thus, the present study aimed to evaluate if the anthocyanins found in Pinot noir pomace extract can affect the target genes related to the Nrf2 signalling pathway in endothelial cells. Our results highlight that the predominant anthocyanin in the Pinot noir pomace extract was malvidin-3-glucoside (3.7 ± 2.7 Eq. Malv-3-glu/kg). Molecular docking indicated that cyanidin-3-glucoside (−6.9 kcal/mol), malvidin-3-glucoside (−6.6 kcal/mol) and peonidin-3-glucoside (−6.6 kcal/mol) showed the highest affinities for the binding sites of the BTB domains in Keap1, suggesting that these components may modify the interaction of this protein with Nrf2. In addition, when HUVEC cells were exposed to different concentrations of Pinot noir pomace extract (100 µg/mL, 200 µg/mL, and 400 µg/mL), no changes in Nrf2 gene expression were observed. However, the gene expression of HO-1 and NQO1, which are in the signalling pathway of this transcription factor, increased according the concentrations of the extract (p = 0.0004 and p = 0.0084, respectively). In summary, our results show that anthocyanins play a very important role in Nrf2 activation and release, while at the same time not promoting its transcription. These preliminary results strongly suggest that the Pinot noir pomace extract can serve as a potent bioactive component source that protects cells against oxidative stress.

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Section: Resultsmentioning

confidence: 99%

Anthocyanins Found in Pinot Noir Waste Induce Target Genes Related to the Nrf2 Signalling in Endothelial Cells

et al. 2022

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“…Presently, one of the researchers used chitosan and alginate to encapsulate bioactive compounds from the waste of grapes, in which, interestingly, the bioactivity of the targeted compound was enhanced and the encapsulation protected the compound from degradation in the gastrointestinal tract [133]. Besides, compounds including peptides are encapsulated using lipid-based nano-carriers or liposomes, which are comprised of double-layer protection of surfactant molecules and aqueous fluid [134]. Furthermore, nano-carriers of hybrid structure (combination of liposome and chitosan) are also in practice to encapsulate compounds like caffeine to enhance the encapsulation efficiency [135].…”

Section: Nanoencapsulation Of Bioactive Compoundsmentioning

confidence: 99%

“…The results indicated that zein loaded with 0.8% rutin concentration reflected slower release (25%) after 60 h as compared to poly (lactic-co-glycolic acid) (100%). Further, in 2021, Portugal researcher Costa et al [134] encapsulated bioactive grape pomace extract using chitosan and alginate nanoparticles, which protected the bioactive compounds from hydrolysis in the gastrointestinal tract as well as enhanced its bioactivity.…”

Section: Polymeric Nano-carriersmentioning

confidence: 99%

Novel Bioactive Extraction and Nano-Encapsulation

et al. 2021

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An extraction technology works on the principle of two consecutive steps that involves mixture of solute with solvent and the movement of soluble compounds from the cell into the solvent and its consequent diffusion and extraction. The conventional extraction techniques are mostly based on the use of mild/high temperatures (50–90 °C) that can cause thermal degradation, are dependent on the mass transfer rate, being reflected on long extraction times, high costs, low extraction efficiency, with consequent low extraction yields. Due to these disadvantages, it is of interest to develop non-thermal extraction methods, such as microwave, ultrasounds, supercritical fluids (mostly using carbon dioxide, SC-CO2), and high hydrostatic pressure-assisted extractions which works on the phenomena of minimum heat exposure with reduced processing time, thereby minimizing the loss of bioactive compounds during extraction. Further, to improve the stability of these extracted compounds, nano-encapsulation is required. Nano-encapsulation is a process which forms a thin layer of protection against environmental degradation and retains the nutritional and functional qualities of bioactive compounds in nano-scale level capsules by employing fats, starches, dextrins, alginates, protein and lipid materials as encapsulation materials.

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“…(a) Physical adsorption includes self-assembly methods such as van der Waals interactions, electrostatic interactions, hydrophobic effects, and affinity recognition [152,153]; (b) Physical entrapment of the particles within the fabric's fibrous structure takes place either by vacuum induction or assisted by intermediary adhesive layers [154][155][156][157]; (c) Covalent bonding comprises short-range intermolecular attractive forces at the molecular scale [158][159][160].…”

Section: Biomedical Applications: Fiber-based Systemsmentioning

confidence: 99%

“…Physical entrapment of the particles within the fabric’s fibrous structure takes place either by vacuum induction or assisted by intermediary adhesive layers [ 154 , 155 , 156 , 157 ];…”

Section: Biomedical Applications: Fiber-based Systemsmentioning

confidence: 99%

Bioactivity of Chitosan-Based Particles Loaded with Plant-Derived Extracts for Biomedical Applications: Emphasis on Antimicrobial Fiber-Based Systems

et al. 2021

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Marine-derived chitosan (CS) is a cationic polysaccharide widely studied for its bioactivity, which is mostly attached to its primary amine groups. CS is able to neutralize reactive oxygen species (ROS) from the microenvironments in which it is integrated, consequently reducing cell-induced oxidative stress. It also acts as a bacterial peripheral layer hindering nutrient intake and interacting with negatively charged outer cellular components, which lead to an increase in the cell permeability or to its lysis. Its biocompatibility, biodegradability, ease of processability (particularly in mild conditions), and chemical versatility has fueled CS study as a valuable matrix component of bioactive small-scaled organic drug-delivery systems, with current research also showcasing CS’s potential within tridimensional sponges, hydrogels and sutures, blended films, nanofiber sheets and fabric coatings. On the other hand, renewable plant-derived extracts are here emphasized, given their potential as eco-friendly radical scavengers, microbicidal agents, or alternatives to antibiotics, considering that most of the latter have induced bacterial resistance because of excessive and/or inappropriate use. Loading them into small-scaled particles potentiates a strong and sustained bioactivity, and a controlled release, using lower doses of bioactive compounds. A pH-triggered release, dependent on CS’s protonation/deprotonation of its amine groups, has been the most explored stimulus for that control. However, the use of CS derivatives, crosslinking agents, and/or additional stabilization processes is enabling slower release rates, following extract diffusion from the particle matrix, which can find major applicability in fiber-based systems within ROS-enriched microenvironments and/or spiked with microbes. Research on this is still in its infancy. Yet, the few published studies have already revealed that the composition, along with an adequate drug release rate, has an important role in controlling an existing infection, forming new tissue, and successfully closing a wound. A bioactive finishing of textiles has also been promoting high particle infiltration, superior washing durability, and biological response.

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Polymeric nanoparticles as oral delivery systems for a grape pomace extract towards the improvement of biological activities

Cited by 30 publications

References 48 publications

Anthocyanins Found in Pinot Noir Waste Induce Target Genes Related to the Nrf2 Signalling in Endothelial Cells

Anthocyanins Found in Pinot Noir Waste Induce Target Genes Related to the Nrf2 Signalling in Endothelial Cells

Novel Bioactive Extraction and Nano-Encapsulation

Bioactivity of Chitosan-Based Particles Loaded with Plant-Derived Extracts for Biomedical Applications: Emphasis on Antimicrobial Fiber-Based Systems

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