Polymeric nanoparticles assembled with microfluidics for drug delivery across the blood-brain barrier

Tavares, Marina Rodrigues; Menezes, Lívia Rodrigues de; Nascimento, Débora Freitas do; Souza, D.H.S.; Reynaud, Franceline; Marques, Maria de Fátima Vieira; Tavares, Maria Inês B.

doi:10.1140/epjst/e2015-50266-2

Cited by 22 publications

(7 citation statements)

References 71 publications

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“…The whole set was placed on a magnetic stirrer adjusted at 37 ± 2 • C and 100 rpm. At appropriate time points (1,2,3,4,6,8,10,12,14,16,20,24, and 28 days), 2 mL aliquot was withdrawn from the release medium and the same volume of fresh medium was added back to maintain a constant volume. Withdrawn samples were diluted with acetone/ethyl acetate mixture (1:1, v/v) and the percentage of PP released was determined spectrophotometrically at 323 nm.…”

Section: In Vitro Release Of Pp From Pnmentioning

confidence: 99%

“…Noteworthy, polymeric nanoparticles (PN) possess a wide range of applications in tissue engineering and drug delivery. Especially for biodegradable and biocompatible types, they can improve bioavailability and safety, control the release of encapsulated drugs [9] as well as overcoming physiological barriers [10]. PCL is a semi-crystalline polyester that is FDA approved as a biodegradable and biocompatible polymer.…”

Section: Introductionmentioning

confidence: 99%

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Long-Acting Paliperidone Parenteral Formulations Based on Polycaprolactone Nanoparticles; the Influence of Stabilizer and Chitosan on In Vitro Release, Protein Adsorption, and Cytotoxicity

et al. 2020

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Long-acting preparations containing the antipsychotic paliperidone for intramuscular injection has drawn considerable attention to achieve harmless long-term treatment. This study aimed to develop paliperidone loaded polycaprolactone (PCL) nanoparticles and investigate the influence of PCL/drug ratio, stabilizer type, and chitosan coating on physicochemical properties, protein adsorption, and cellular toxicity. Results showed that chitosan coating produced enlarged particle sizes, shifted the surface charges from negative into positive and did not influence encapsulation efficiencies. Chitosan coating relatively sustained the drug release especially in pluronic stabilized formulations. Pluronic F127 based formulations exhibited the least protein adsorption (384.3 μg/mL). Chitosan coating of Tween 80 and polyvinyl alcohol stabilized formulations significantly (p < 0.05) increased protein adsorption. Cellular viability was concentration-dependent and negatively affected by stabilizers. All formulations did not show cellular death at 1.56 μg/mL. Inflammatory responses and oxidative stress were less affected by Tween 80 compared with other stabilizers. Chitosan minimized all aspects of cellular toxicity. Collectively, stabilizer type and chitosan coating play critical roles in developing safe and effective long-acting PCL nanoparticles intended for parenteral drug delivery. The coated formulations containing Tween 80 and Pluronic F127 as stabilizers are warranted a future in vivo study to delineate its safety and efficacy profiles.

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Section: In Vitro Release Of Pp From Pnmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Long-Acting Paliperidone Parenteral Formulations Based on Polycaprolactone Nanoparticles; the Influence of Stabilizer and Chitosan on In Vitro Release, Protein Adsorption, and Cytotoxicity

et al. 2020

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“…The BBB is a physiological barrier of the central nervous system (CNS), which regulates the transport system between the brain and blood. The BBB disturbs the passage of drugs to the brain and restricts effective therapy for CNS diseases [ 151 ]. Additionally, the BBB can be a particular target because the malfunction of the BBB caused by neuronal diseases results in additional conditions [ 152 ].…”

Section: Applicationsmentioning

confidence: 99%

Vasculature-On-A-Chip for In Vitro Disease Models

et al. 2017

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Vascularization, the formation of new blood vessels, is an essential biological process. As the vasculature is involved in various fundamental physiological phenomena and closely related to several human diseases, it is imperative that substantial research is conducted on characterizing the vasculature and its related diseases. A significant evolution has been made to describe the vascularization process so that in vitro recapitulation of vascularization is possible. The current microfluidic systems allow elaborative research on the effects of various cues for vascularization, and furthermore, in vitro technologies have a great potential for being applied to the vascular disease models for studying pathological events and developing drug screening platforms. Here, we review methods of fabrication for microfluidic assays and inducing factors for vascularization. We also discuss applications using engineered vasculature such as in vitro vascular disease models, vasculature in organ-on-chips and drug screening platforms.

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“…Therefore, the researcher needs to pay attention to the temperatures used so as not to degrade the polymer during extrusion and also must pay attention to the time necessary for the nanoparticles to disperse properly. For natural polymers and few biopolymers, the degradation and melting temperatures are very close [7,12,17]. A typical extruder is shown in Figure 3.…”

Section: Melt Extrusionmentioning

confidence: 96%

Polymer Nanocomposites

Tavares¹,

Silva²,

Silva³

et al. 2017

Nanostructured Materials - Fabrication to Applications

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The development of polymer nanocomposites has advanced, especially due to their new properties after nanoparticle incorporation. Many nanocomposites composed of synthetic polymers and/or biopolymers have been studied after incorporation of a diversity of nanoparticles, which differ in form, shape, surface area and chemical organization.In this chapter, some examples of nanocomposites based on poly-vinyl alcohol (PVA); polycarbonate (PC) and matrixes of dental resins are presented. These nanocomposites could be obtained by three basic methods: in situ polymerization, solution casting and melt extrusion. The best method is determined by the relation and route to the polymernanoparticle pair. The dispersion and distribution of nanoparticles in the polymer matrix is the key to obtaining new materials with synergism of compounds properties. This synergism depends on how strong is the intermolecular interaction between the polymer matrix and nanoparticles. The evaluation of new nano systems can be done by different techniques, usually microscopy, X-ray diffraction, thermal analysis and so on. Low-field NMR relaxometry has been used to evaluate polymer nanocomposites. This technique provides valuable information related to the interaction of the nanoparticles with the polymer matrix, and it also indicates the dispersion and distribution of these nanoparticles in the matrix.

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Polymeric nanoparticles assembled with microfluidics for drug delivery across the blood-brain barrier

Cited by 22 publications

References 71 publications

Long-Acting Paliperidone Parenteral Formulations Based on Polycaprolactone Nanoparticles; the Influence of Stabilizer and Chitosan on In Vitro Release, Protein Adsorption, and Cytotoxicity

Long-Acting Paliperidone Parenteral Formulations Based on Polycaprolactone Nanoparticles; the Influence of Stabilizer and Chitosan on In Vitro Release, Protein Adsorption, and Cytotoxicity

Vasculature-On-A-Chip for In Vitro Disease Models

Polymer Nanocomposites

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