Polymeric vehicles for transport and delivery of DNA via cationic micelle template method

Haladjova, Emi; Kyulavska, Mariya; Doumanov, Jordan; Topouzova-Hristova, Tanya; Petrov, Petar

doi:10.1007/s00396-017-4193-7

Cited by 12 publications

(12 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In summary, our results show for the first time that the here tested CPMs, previously applied by us as gene delivery vectors with low cytotoxicity [16,17], not only can be successful for biofilm dispersal and removal, but exert also activity as bacterial cell-envelope permeabilizing agents. For internal medical applications, the low stability of the particles in saline or microbiological media (data not shown) appears an obstacle.…”

mentioning

confidence: 57%

“…The micelles formed thereof have previously been used as gene delivery vector systems manifesting low cytotoxicity to eukaryotic cells and high cells internalization [16,17]. Block copolymers based on quaternized poly (2-vinylpyridine) (PQVP) and poly (2-(dimethylamino)ethyl methacrylate) (PDMAEMA) cationic moieties were used.…”

mentioning

confidence: 99%

“…Block copolymers based on quaternized poly (2-vinylpyridine) (PQVP) and poly (2-(dimethylamino)ethyl methacrylate) (PDMAEMA) cationic moieties were used. The micelles formed thereof have previously been used as gene delivery vector systems manifesting low cytotoxicity to eukaryotic cells and high cells internalization [16,17]. Therefore, they were considered as promising systems for the dispersal of bacterial biofilms.…”

mentioning

confidence: 99%

“…The polystyrene-b-poly (quaternized 2-vinylpyridine) (PS-PQVP) block copolymers were synthesized by anionic polymerization following the procedure described elsewhere [18]. The poly(2-(dimethylamino)ethyl methacrylate)-b-poly(ε-caprolactone)-b-poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA-PCL-PD-MAEMA) triblock copolymers were synthesized by ATRP according to procedures reported previously [17]. The molecular characteristics of the copolymers were as follows: PS-PQVP block copolymers (Mn = 69000 g/mol, Đ = 1.08, PQVP content 77 wt% and Mn = 202000 g/mol, Đ = 1.02, PQVP content 75 wt%) denoted as PS-PQVP 35K and PS-PQVP 115K; PDMAEMA-PCL-PDMAEMA triblock copolymers (Mn = 14300 g/mol, Đ = 1.78, PDMAEMA content of 44 wt% and Mn = 17100 g/mol, Đ = 1.20, PDMAEMA content of 53 wt%,) denoted as MKPa4 and MKPa12, respectively.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Application of cationic polymer micelles for the dispersal of bacterial biofilms

Borisova

Haladjova

Kyulavska

et al. 2018

Engineering in Life Sciences

Self Cite

View full text Add to dashboard Cite

Contamination of surfaces in hospitals and food industry by bacterial biofilms is a serious health risk. Of concern is their resistance to routine antibacterials and disinfectants. This requires the development of novel approaches to biofilm detachment. The study evaluates the effectiveness of cationic polymer micelles (CPMs) against pre‐formed biofilms. CPMs based on different polycations were used. The hydrodynamic radius of the particles ranged from 16 to 360 nm. Biofilms of Escherichia coli 420, Pseudomonas aeruginosa PAO1, Staphylococcus aureus 29213 and Bacillus subtilis 168 were cultivated for 24 h then the pre‐formed biofilms were treated with the CPMs for 2, 4 or 6 h. Biofilm biomass was evaluated by the crystal violet assay, and live/dead fluorescence test was applied for bacterial viability. The ability of CPMs to interact with pre‐formed biofilms of the model strains was evaluated. We observed that the most effective CPMs were those based on poly(2‐(dimethylamino)ethyl methacrylate) copolymers which reduced the biofilm biomass three‐ to four‐fold compared with the treatment of the biofilm with water. Significantly reduced vitality of the bacteria in the biofilms was registered by the live/dead stain. The results indicate the applicability of the CPMs for disinfection of biofilm‐contaminated surfaces and the treatment of wounds.

show abstract

mentioning

confidence: 57%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Application of cationic polymer micelles for the dispersal of bacterial biofilms

Borisova

Haladjova

Kyulavska

et al. 2018

Engineering in Life Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…Owing to high positive net charge on the surface (ξ= 51.7 mV), these micelles were used for the adhesion of negatively charged plasmid DNA (ξ = −32.3 mV) prior to the seeded radical polymerization of N‐isopropylacrylamide. The use of cationic polyplexes as a template for capsule fabrication to deliver DNA was also reported by Haladjova and co‐workers . In a recent report, Sundaramurthy and Sundramoorthy presented the fabrication of polyelectrolyte capsules comprised of multiple layers of alginate and 3‐carboxyphenylboronic acid modified chitosan oligosaccharide through LbL deposition for the delivery of insulin …”

Section: Common Methods For Preperation Of Polymeric Hollow Capsulesmentioning

confidence: 80%

Current Advances of Hollow Capsules as Controlled Drug Delivery Systems

Tran

Bhave

2020

ChemistrySelect

View full text Add to dashboard Cite

Polymeric hollow capsules have attracted increasing interest for the development of controlled drug delivery systems due to their capacities to load high amount of drugs and be able to finely tune the drug release profile. Various methods and strategies for capsule preparation and drug loading have been developed over time. Besides that, the controlled drug release from hollow capsules upon external and internal triggers is of great interest and has been a research focus in this area. This article aims to give the most recent progress review on hollow capsule based controlled drug delivery systems with a highlight on strategies being used for drug loading and stimuli-release. In detail, the fabrication of hollow capsules using templateassisted or template-free methods will be introduced first. This will be followed by current strategies for pre-loading and postloading of therapeutic agents into capsules. The article will then be particularly focused on discussing diverse drug-release systems corresponding to various stimuli conditions including temperature, pH, ultrasound, light irradiation and enzyme degradation.

show abstract

Micropattern‐Controlled Cell Density and Its Effect on Gene Transfection of Mesenchymal Stem Cells

Wang

Yoshitomi

Naoki

et al. 2022

Adv Materials Inter

View full text Add to dashboard Cite

permeation through the cell membrane is difficult. [10,11] Various viral-mediated transfection and nonviral transfection methods have been developed for the efficient delivery of exogenous genes into cells. [12,13] The former method generally has high transfection efficiency, while the latter method has low immunogenicity and is relatively safe. [14,15] Many cationic synthesized carriers, including polyethylenimine, dendrimers, peptides, and lipids, have been designed and used to increase the transfection efficiency of nonviral transfection. [16][17][18][19][20] In addition to gene transfection vectors and methods, the cellular microenvironment can also affect gene transfection. [21][22][23][24] Cells in tissues and organs are surrounded by neighboring cells and extracellular matrices (ECMs). [25][26][27] Cells interact with their microenvironment and neighboring cells. [28,29] Cells show different morphology depending on cell-ECM and cell-cell interactions in different tissues and organs. [30] To determine the relevance of cell morphology to cell functions, researchers have precisely controlled the cell geometry, aspect ratio, spreading area, adhesion area, and chirality of single cells by micropatterned surfaces. [31][32][33][34][35][36][37] Through the versatile micropatterning technique, cell morphology has been demonstrated to be important in controlling cell adhesion, proliferation, and stem cell differentiation. [38][39][40][41][42] The influence of cell morphology on gene transfection has also been recently reported. Large cells with a high aspect ratio and more adhesion areas are preferable for gene transfection. [43] Compared to single cells, individual cells in cell populations have a more complex microenvironment. [44] Both cell morphology and cell-cell interactions in the cell population should be considered to determine the influence of the microenvironment on cell functions. [45] Cell morphology and cell-cell interactions are dependent on cell density. [33] Therefore, cell density in the cell population can affect not only individual cell morphology but also cellcell interactions. [46] High cell density mass in pellet culture has been reported to promote chondrogenic differentiation of stem cells. [47,48] High cell density (112.5 × 10 3 cells cm −2 ) has been reported to promote chondrogenic and adipogenic differentiation of bone marrow-derived mesenchymal stem cells. [44] Cell density can also affect cell viability and gene transfection. Optimization of cell density is recommended to maximize transfection efficiency.

show abstract

Polymeric vehicles for transport and delivery of DNA via cationic micelle template method

Cited by 12 publications

References 41 publications

Application of cationic polymer micelles for the dispersal of bacterial biofilms

Application of cationic polymer micelles for the dispersal of bacterial biofilms

Current Advances of Hollow Capsules as Controlled Drug Delivery Systems

Micropattern‐Controlled Cell Density and Its Effect on Gene Transfection of Mesenchymal Stem Cells

Contact Info

Product

Resources

About