Polymerization of Bisphenol A by Purified Laccase from Trametes villosa

Uchida, Hiroyuki; Fukuda, Tsuguo; Miyamoto, Hideo; Kawabata, Takahiro; Suzuki, Motoshi; Uwajima, Takayuki

doi:10.1006/bbrc.2001.5593

Cited by 117 publications

(55 citation statements)

References 9 publications

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“…A reaction mixture containing 0.01% bisphenol A and 3.6 U mL À1 of Lac 1 in 50 mM citrate buffer (pH 4.0) was incubated at 28 C for 24 h. A water-insoluble precipitate had been formed after the reaction without HBT, probably due to the formation of polymerized products as previously reported. 3,14) To identify the reaction product in the aqueous solution, the reaction mixture was centrifuged at 14;000 g for 15 min to remove the water-insoluble precipitate, and then the reaction products in the supernatant were extracted with ethyl acetate. The ethyl acetate layer was evaporated to dryness, and the resulting residue was redissolved in 100 mL of ethyl acetate.…”

Section: )mentioning

confidence: 99%

Decolorization of Synthetic Dyes and Biodegradation of Bisphenol A by Laccase from the Edible Mushroom,Grifola frondosa

Nitheranont

Watanabe

Suzuki

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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Section: )mentioning

confidence: 99%

Decolorization of Synthetic Dyes and Biodegradation of Bisphenol A by Laccase from the Edible Mushroom,Grifola frondosa

Nitheranont

Watanabe

Suzuki

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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“…4,5 This paper describes further progress in identification of the BPA reaction products and removal of BPA's estrogenic activity by the laccase.…”

Section: Introductionmentioning

confidence: 99%

Transformation products of bisphenol A by a recombinant Trametes villosa laccase and their estrogenic activity

Fukuda¹,

Uchida²,

Suzuki

et al. 2004

J of Chemical Tech & Biotech

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A Trametes villosa laccase, produced as a recombinant protein in Aspergillus oryzae, was purified to an electrophoretically homogeneous state and employed in studies on the metabolism of bisphenol A. Structural analysis of the bisphenol A reaction products by the enzyme indicated that the dimer, trimer, tetramer, pentamer, and hexamer of bisphenol A with C-C and/or C-O bonds between phenol moieties, were formed as the result of successive oxidative-condensation. The reaction mixture also contained oligomers fragments, each with phenol molecules, suggesting the occurrence of cleavage of the formed oligomers to release 4-isopropenylphenol. A luciferase reporter assay using COS-7 cells revealed that both the soluble and insoluble fractions of the bisphenol A reaction products had no estrogenic activity even at rather high concentrations.

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“…Kang and Kondo (2002) and Ike et al (2002b) showed the degradation of BPA by bacteria isolated from river water and/or activated sludge. Hirano et al (2000) reported the enzymatic degradation and Uchida et al (2001) the polymerization of BPA.…”

Section: Introductionmentioning

confidence: 99%

Absorption, Translocation and Metabolism of Bisphenol A, a Possible Endocrine Disruptor, in Rice Seedlings

Noureddin

Furumoto

Yamagishi

et al. 2004

ecb

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Rice seedlings quickly absorbed bisphenol A (BPA), a possible endocrine disruptor, in culture medium, indicating that they are capable of improving water quality. Measurements of BPA extracted from roots with the stem base and leaves/stems indicated that BPA was translocated from the roots to the top of the plants. The recovery rate by McOH extraction was very low even during the absorption period, indicating that BPA is not bioaccumulated in the seedlings. The roots and tops of the rice seedlings cultured in the medium containing deuterium-labeled BPA, [2H] BPA, were extracted with MeOH and hydrolyzed with HC1 and NaOH. The extracts and the hydrolyzates were analyzed by 2H-NMR. The spectral data indicated that about 5% of the metabolites, as [2H] BPA equivalents, was found in the McOH extracts and ca. 25% in the acidic and alkaline hydrolyzates. The rest (ca. 70%) could be polymerized and/or bound in the tissues as insoluble, unhydrolyzable forms. The signals in the 2H-NMR spectra of the extracts and the hydrolyzates indicated that BPA was metabolized by oxidation and skeletal rearrangement to several compounds.

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Polymerization of Bisphenol A by Purified Laccase from Trametes villosa

Cited by 117 publications

References 9 publications

Decolorization of Synthetic Dyes and Biodegradation of Bisphenol A by Laccase from the Edible Mushroom,Grifola frondosa

Decolorization of Synthetic Dyes and Biodegradation of Bisphenol A by Laccase from the Edible Mushroom,Grifola frondosa

Transformation products of bisphenol A by a recombinant Trametes villosa laccase and their estrogenic activity

Absorption, Translocation and Metabolism of Bisphenol A, a Possible Endocrine Disruptor, in Rice Seedlings

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