Polymerized phospholipid bilayers as permanent coatings for small amine separations using mixed aqueous/organic capillary zone electrophoresis

Pei, Lei; Lucy, Charles A.

doi:10.1016/j.chroma.2012.07.017

Cited by 10 publications

(6 citation statements)

References 68 publications

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“…Capillary electrophoresis (CE) is increasingly used for the analysis of a wide variety of analytes due to its speed and efficiency. [22][23][24][25] Compared to HPLC, CE has high resolving power, low cost operation, and simplicity in design, optimization and operation. 26 CE is also appropriate as a portable instrument as it has limited solvent consumption and lower weight.…”

Section: Introductionmentioning

confidence: 99%

Determination of inorganic anions in oilfield water using capillary electrophoresis with indirect fluorescence detection

Pei

Schmidt²,

Crabtree³

et al. 2015

Anal. Methods

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Section: Introductionmentioning

confidence: 99%

Determination of inorganic anions in oilfield water using capillary electrophoresis with indirect fluorescence detection

Pei

Schmidt²,

Crabtree³

et al. 2015

Anal. Methods

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“…Several polymeric materials, including poly(ethylene oxide), polydimethylacrylamide, cellulose derivatives, and various polyelectrolytes have been widely used for dynamically coating capillaries to reduce adsorption in CE. Strongly charged high molecular weight polyelectrolytes have been shown to give uniform repeatable film coatings , characterized by a good stability due to strong interactions between oppositely charged polyelectrolytes and extensive interpenetrations between layers . We thus chose to investigate a five successive multiple ionic‐polymer layers coating (SMIL) consisting of poly(diallyldimethylammonium) chloride (PDADMAC)/poly(sodium 4‐styrenesulfonate (PSS) (the last layer being cationic) .…”

Section: Introductionmentioning

confidence: 99%

“…Covalent capillary coating are obtained by derivatization of the silanol groups of the capillary surface. However, preparing the covalently coated capillary is rather labor intensive and time consuming and the use of polymers that can be physically adsorbed onto the capillary is a more effective and attractive method to reduce adsorption . Several polymeric materials, including poly(ethylene oxide), polydimethylacrylamide, cellulose derivatives, and various polyelectrolytes have been widely used for dynamically coating capillaries to reduce adsorption in CE.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the enantioseparation of a diaryl‐pyrazole sulfonamide derivative by capillary electrophoresis in a dual CD mode using experimental design

et al. 2014

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A CE method using dual cationic and neutral cyclodextrins (CD) was optimized for the enantiomeric separation of a compound presenting a diaryl sulfonamide group. Preliminary studies were made to select the optimal CDs and pH of the BGE. Two CDs (amino-β-CD and β-CD) were selected to separate the enantiomers in a 67 mM phosphate buffer at pH 7.4. However, the repeatability of the analyses obtained on bare-fused silica capillary was not acceptable owing to the adsorption of the amino-β-CD to the capillary. To prevent this, a dynamic coating of the capillary was used employing five layers of ionic-polymer (poly(diallyldimethylammonium) chloride (PDADMAC) and poly(sodium 4-styrenesulfonate). The efficiency of the coating was assessed by measuring the EOF stability. Repeatability of the injections was obtained when intermediate coating with PDADMAC was performed between each run. Secondly, this enantioseparation method was optimized using a central composite circumscribed design including three factors: amino-β-CD and β-CD concentrations and the percentage of methanol. Under the optimal conditions (i.e. 16.6 mM of amino-β-CD, 2.6 mM of β-CD, 0% MeOH in 67 mM phosphate buffer (pH 7.4) as BGE, cathodic injection 0.5 psi, 5 s, separation voltage 15 kV and a temperature of 15°C), complete enantioresolution of the analyte was obtained. It is worth mentioning that the design of experiments (DOE) protocol employed showed a significant interaction between CDs, highlighting the utility of DOE in method development. Finally, small variations in the ionic-polymer concentrations did not significantly influence the EOF, confirming the robustness of the coating method.

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“…However, once polymerized, the lipids showed significantly greater stability, with the bilayer coatings remaining stable for over 1500 or 600 capillary volumes for methanol or acetonitrile, respectively. Additionally, the polymerized DiynePC coating was stable after 4 months of dried storage [24]. …”

Section: Introductionmentioning

confidence: 99%

Stabilized phospholipid membranes in chromatography: toward membrane protein-functionalized stationary phases

2014

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Transmembrane protein (TMP)-functionalized analytical platforms represent a powerful new paradigm in chemical analysis. Of particular interest is the development of high throughput, TMP-functionalized stationary phases for affinity chromatography of complex analyte libraries. A number of natural and synthetic phospholipids and lipid-mimics have been utilized for TMP reconstitution, though the resulting membranes often lack the requisite chemical and temporal stability for long term utilization, a problem that is exacerbated in flowing separation systems. Over the past two decades, polymerizable lipids have been developed that exhibit markedly increased membrane stability, while still supporting TMP function. More recently, these lipids have been incorporated into a range of analytical platforms, including separation-based platforms, and are now poised to make significant impacts in TMP-based separations. Here, we describe the current methods for preparing TMP-containing stationary phases and examine the potential utility of polymerizable lipids in TMP affinity chromatography.

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Polymerized phospholipid bilayers as permanent coatings for small amine separations using mixed aqueous/organic capillary zone electrophoresis

Cited by 10 publications

References 68 publications

Determination of inorganic anions in oilfield water using capillary electrophoresis with indirect fluorescence detection

Determination of inorganic anions in oilfield water using capillary electrophoresis with indirect fluorescence detection

Optimization of the enantioseparation of a diaryl‐pyrazole sulfonamide derivative by capillary electrophoresis in a dual CD mode using experimental design

Stabilized phospholipid membranes in chromatography: toward membrane protein-functionalized stationary phases

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