Polymerized Planar Suspended Lipid Bilayers for Single Ion Channel Recordings: Comparison of Several Dienoyl Lipids

Heitz, Benjamin A.; Xu, Juhua; Jones, Ian W.; Keogh, John P.; Comi, Troy J.; Hall, H. K.; Aspinwall, Craig A.; Saavedra, S. Scott

doi:10.1021/la1025944

Cited by 26 publications

(56 citation statements)

References 67 publications

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“…In fact, it seems that nearly the full spectrum of polymerizable groups suited for radical polymerization has been used to make polyzwitterions in the past, including not only styrenes, vinylpyridines, vinylimidazoles or vinylesters [124], but also rather uncommon polymerizable groups such as dienoic acids [123,124,[131][132][133][134][135][136][137][138][139], vinylcyclopropanes [124,134] or isocyanides [69], and cyclopolymerizing species such as diallylammonium [64,87,88,124,134,[140][141][142][143][144][145][146][147][148][149][150][151][152][153] and closely related divinyl monomers [93,94]. Moreover, a number of monomers confined exclusively to (often alternating) radical copolymerization, such as vinylethers [124,134], fumarates and maleates [124,134,[154][155][156], isobutylenes [157] and ...…”

Section: Synthesis By Chain Growth Polymerizationsmentioning

confidence: 99%

Structures and Synthesis of Zwitterionic Polymers

2014

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Abstract:The structures and synthesis of polyzwitterions ("polybetaines") are reviewed, emphasizing the literature of the past decade. Particular attention is given to the general challenges faced, and to successful strategies to obtain polymers with a true balance of permanent cationic and anionic groups, thus resulting in an overall zero charge. Also, the progress due to applying new methodologies from general polymer synthesis, such as controlled polymerization methods or the use of "click" chemical reactions is presented. Furthermore, the emerging topic of responsive ("smart") polyzwitterions is addressed. The considerations and critical discussions are illustrated by typical examples.

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Section: Synthesis By Chain Growth Polymerizationsmentioning

confidence: 99%

Structures and Synthesis of Zwitterionic Polymers

2014

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“…30–32,36–39 Here lateral diffusion in unpolymerized and UV polymerized PSLBs formed from dienoyl lipids was characterized using FRAP. The variables examined included the location and number of polymerizable groups in the lipid tails and the effect of polymerization above and below the main phase transition temperature ( T m ).…”

Section: Introductionmentioning

confidence: 99%

Photopolymerization of Dienoyl Lipids Creates Planar Supported Poly(lipid) Membranes with Retained Fluidity

et al. 2016

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Polymerization of substrate-supported bilayers composed of dienoyl phosphatidylcholine (PC) lipids is known to greatly enhance their chemical and mechanical stability, however the effects of polymerization on membrane fluidity have not been investigated. Here planar supported lipid bilayers (PSLBs) composed of dienoyl PCs on glass substrates were examined to assess the degree to which UV-initiated polymerization affects lateral lipid mobility. Fluorescence recovery after photobleaching (FRAP) was used to measure the diffusion coefficients (D) and mobile fractions of rhodamine-DOPE in unpolymerized and polymerized PSLBs composed of bis-sorbyl phosphatidylcholine (bis-SorbPC), mono-sorbyl phosphatidylcholine (mono-SorbPC), bis-dienoyl phosphatidylcholine (bis-DenPC) and mono-dienoyl phosphatidylcholine (mono-DenPC). Polymerization was performed in both the Lα and Lβ phase for each lipid. In all cases, polymerization reduced membrane fluidity, however measurable lateral diffusion was retained which is attributed to a low degree of polymerization. The D values for sorbyl lipids were less than those of the denoyl lipids; this may be a consequence of the distal location of polymerizable group in the sorbyl lipids which may facilitate inter-leaflet bonding. The D values measured after polymerization were 0.1 to 0.8 of those measured before polymerization, a range that corresponds to fluidity intermediate between that of a Lα phase and a Lβ phase. This D range is comparable to ratios of D values reported for liquid-disordered (Ld) and liquid-ordered (Lo) lipid phases, and indicates that the effect of UV polymerization on lateral diffusion in a dienoyl PSLB is similar to the transition from a Ld phase to a Lo phase. The partial retention of fluidity in UV polymerized PSLBs, their enhanced stability, and the activity of incorporated transmembrane proteins and peptides is discussed.

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“…Full realization of nanopore sequencing's potential will require additional progress in areas including nanopore parallelization 26 , channel setup 27–30 and microfluidics 31 .…”

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confidence: 99%

Decoding long nanopore sequencing reads of natural DNA

et al. 2014

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Nanopore sequencing of DNA is a single-molecule technique that may achieve long reads, low cost and high speed with minimal sample preparation and instrumentation. Here, we build on recent progress with respect to nanopore resolution and DNA control to interpret the procession of ion current levels observed during the translocation of DNA through the pore MspA. As approximately four nucleotides affect the ion current of each level, we measured the ion current corresponding to all 256 four-nucleotide combinations (quadromers). This quadromer map is highly predictive of ion current levels of previously unmeasured sequences derived from the bacteriophage phi X 174 genome. Furthermore, we show nanopore sequencing reads of phi X 174 up to 4,500 bases in length that can be unambiguously aligned to the phi X 174 reference genome, and demonstrate proof-of-concept utility with respect to hybrid genome assembly and polymorphism detection. This work provides the foundation for nanopore sequencing of long, complex, natural DNA strands.

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Polymerized Planar Suspended Lipid Bilayers for Single Ion Channel Recordings: Comparison of Several Dienoyl Lipids

Cited by 26 publications

References 67 publications

Structures and Synthesis of Zwitterionic Polymers

Structures and Synthesis of Zwitterionic Polymers

Photopolymerization of Dienoyl Lipids Creates Planar Supported Poly(lipid) Membranes with Retained Fluidity

Decoding long nanopore sequencing reads of natural DNA

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