Polymethylmethacrylate (PMMA) as an Embedding Medium Preserving Tissues and Foreign Materials Encroaching in Endovascular Devices

Guidoin, Robert; Zhang, Ze; Douville, Yvan; Basle, Michel‐Félix; Grizon, F.; Маринов, Г.; Zarins, Christopher K.; Legrand, A. P.; Guzman, Randolph

doi:10.1080/10731190600684041

Cited by 3 publications

(2 citation statements)

References 49 publications

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“…Each vascular segment was embedded in plastic, cross‐sectioned, and stained. Inclusion of anchor and tissues in polymethymethacrylate permitted thin slides and preserved the capacity to perform the appropriate staining . The vascular segment was cross‐sectioned in a minimum of four segments.…”

Section: Methodsmentioning

confidence: 99%

Chronic Implantation of Intravascular Cardioverter Defibrillator in a Canine Model: Device Stability, Vascular Patency, and Anchor Histology

Merkely

Molnár

Gellér

et al. 2013

Pacing Clinical Electrophis

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Section: Methodsmentioning

confidence: 99%

Chronic Implantation of Intravascular Cardioverter Defibrillator in a Canine Model: Device Stability, Vascular Patency, and Anchor Histology

Merkely

Molnár

Gellér

et al. 2013

Pacing Clinical Electrophis

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“…This technique is well adapted to metallic implants and was successfully employed in research in bones and=or bone substitutes together or not with metallic implants [29,30]. Details are given elsewhere [31].…”

Section: Histologymentioning

confidence: 99%

MRI Virtual Biopsies: Analysis of an Explanted Endovascular Device and Perspectives for the Future

Guidoin¹,

Zhang²,

Douville³

et al. 2006

Artificial Cells, Blood Substitutes, and Biotechnology

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Information that can be obtained by magnetic resonance imaging (MRI) of explanted endovascular devices must be validated as this method is non-destructive. Histology of such a device together with its encroached tissues can be elegantly performed after polymethymethacrylate (PMMA) embedding, but this approach requires destruction of the specimen. The issue is therefore to 241 determine if the MRI is sufficient to fully validate an explanted device based upon the characterization of an explanted specimen.An AneuRx device deployed percutaneously 25 months earlier in a 75-yearold patient was removed en bloc at autopsy together with the surrounding aneurysmal sac and segments of the upstream and downstream arteries. Macroscopic pictures were taken and a slice of the cross-section was processed for histology after polymethylmethacrylate (PMMA) embedding. For the magnetic resonance imaging investigation, the device was inserted in a Biospec 4.7 T MRI system with a 20 mm diameter birdcage resonator used for both emission and reception. A Spin-Echo (SE) was used to acquire both T1 proton density (PD) and T2 weighted images. A gradient-echo (GE) sampling of a free induction decay (GESFID) was used to generate multiple GE images using a single excitation pulse so that four images at different TE were obtained in the same acquisition.The selected explanted device was outstandingly well-healed compared to most devices harvested from humans. No inflammatory process was observed in contact or at distance of the materials. In MRI T1 images display no specific contrast and were homogeneous in the different tissues. The contrast was improved on proton density weighed images. On the T2 weighed images, the different areas were well indentified. The diffusion images displayed in the surrounding B region had the greatest diffusion coefficient and the greatest anisotropy.The MRI analysis of the explanted AneuRx device illustrates the possibilities of this technique to characterize the interaction of the endovascular graft with the surrounding tissues. MRI is a breakthrough to investigate explanted medical devices but it also can be advantageously used in vivo to obtain virtual biopsies, because real biopsies to determine the 3 Bs (biocompatibility, biofunctionality and bioresilience) cannot be carried out as they could obviously initiate infection and degradation of the foreign materials.

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Analysis of Explanted Latecba Modular Stent-grafts Deployed Transrenally to Repair AAAs with Short Necks in 29 Dogs

Guidoin

Peirano

Barone³

et al. 2009

Artificial Cells, Blood Substitutes, and Biotechnology

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Twenty-nine modular stent-grafts deployed transrenally to repair AAAs with short necks in dogs were harvested at autopsy of the animals after scheduled durations of implantations of 10 days, one month, three months, and six months. Analyses of the explanted devices included non-destructive techniques such as gross observations, X-rays CT scan, IVUS and angioscopy. Further to appropriate dissection, histological investigations were carried out by means of scanning electron microscopy (SEM) and light microscopy. All the 29 specimens were extensively encapsulated with fibrous tissues but the fibrous capsule was thin in six of them; four capsules were ulcerated. The X-rays confirmed the stability of the devices that were still straight (12), slightly bent (12) or bent (4). The modules were misaligned in only one case. IVUS and angioscopy confirmed the patency of all the stent-grafts with thin internal capsules both proximally and distally with variable capsulation in the mid-section of the grafts. The left renal artery orifices were found to be patent at dissection with no obstruction to flow. The luminal flow surface of the stent-grafts was smooth and glistening proximally and distally containing endothelial like cells and vasa-vasorum. Poor healing was noted in the aneurysm area. Transrenal deployment of this modular stent-graft is feasible and gave excellent results with regard to biofunctionality and biocompatibility. The device proved to be safe and efficient.

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Polymethylmethacrylate (PMMA) as an Embedding Medium Preserving Tissues and Foreign Materials Encroaching in Endovascular Devices

Cited by 3 publications

References 49 publications

Chronic Implantation of Intravascular Cardioverter Defibrillator in a Canine Model: Device Stability, Vascular Patency, and Anchor Histology

Chronic Implantation of Intravascular Cardioverter Defibrillator in a Canine Model: Device Stability, Vascular Patency, and Anchor Histology

MRI Virtual Biopsies: Analysis of an Explanted Endovascular Device and Perspectives for the Future

Analysis of Explanted Latecba Modular Stent-grafts Deployed Transrenally to Repair AAAs with Short Necks in 29 Dogs

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