Polymorph and morphology of calcium carbonate crystals induced by proteins extracted from mollusk shell

Feng, Qingling; Pu, Guangying; Pei, Yukui; Cui, Fuzhai; Li, H.D; Kim, T.N

doi:10.1016/s0022-0248(00)00396-1

Cited by 105 publications

(78 citation statements)

References 19 publications

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“…[71] This test has been performed with soluble matrices extracted from both calcite and aragonite. [72][73][74][75] The test was adapted as follows: different amounts of ASM (0.1 mg mL À1 to 20 mg mL À1 ) in CaCl 2 solution (10 mm, 500 mL) were introduced onto an 8-well culture slide (BD Falcon). The culture slide was closed with a plastic cover, which had been pierced with 1 mm holes.…”

Section: Methodsmentioning

confidence: 99%

Evolution of Nacre: Biochemistry and Proteomics of the Shell Organic Matrix of the Cephalopod Nautilus macromphalus

Marie¹,

Marin²,

Marie³

et al. 2009

ChemBioChem

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Matrix evolutions: We have biochemically characterized the nacre matrix of the cephalopod Nautilus macromphalus, in part by a proteomic approach applied to the acetic acid‐soluble and ‐insoluble shell matrices, as well as to spots obtained after 2D gel electrophoresis. Strikingly, most of the obtained partial sequences are entirely new, whereas a few correspond only partly with bivalvian nacre proteins. Our findings shed new light on the macroevolution of nacre matrix proteins. magnified imageIn mollusks, one of the most widely studied shell textures is nacre, the lustrous aragonitic layer that constitutes the internal components of the shells of several bivalves, a few gastropods, and one cephalopod: the nautilus. Nacre contains a minor organic fraction, which displays a wide range of functions in relation to the biomineralization process. Here, we have biochemically characterized the nacre matrix of the cephalopod Nautilus macromphalus. The acid‐soluble matrix contains a mixture of polydisperse and discrete proteins and glycoproteins, which interact with the formation of calcite crystals. In addition, a few bind calcium ions. Furthermore, we have used a proteomic approach, which was applied to the acetic acid‐soluble and ‐insoluble shell matrices, as well as to spots obtained after 2D gel electrophoresis. Our data demonstrate that the insoluble and soluble matrices, although different in their bulk monosaccharide and amino acid compositions, contain numerous shared peptides. Strikingly, most of the obtained partial sequences are entirely new. A few only partly match with bivalvian nacre proteins. Our findings have implications for knowledge of the long‐term evolution of molluskan nacre matrices.

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Section: Methodsmentioning

confidence: 99%

Evolution of Nacre: Biochemistry and Proteomics of the Shell Organic Matrix of the Cephalopod Nautilus macromphalus

Marie¹,

Marin²,

Marie³

et al. 2009

ChemBioChem

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“…Crystal growth studies with acidic protein mixtures and protein fractions isolated from invertebrate biominerals suggest that these proteins function to stabilize amorphous calcium carbonate, 20,21 nucleate crystalline phases, 22,23 template crystal orientation, 22 and modulate crystal morphology. [24][25][26][27] However, to ultimately assign specific roles to individual acidic proteins, they must first be effectively isolated from biomineralized composites.…”

Section: Introductionmentioning

confidence: 99%

CaCO₃Biomineralization: Acidic 8-kDa Proteins Isolated from Aragonitic Abalone Shell Nacre Can Specifically Modify Calcite Crystal Morphology

et al. 2005

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Acidic proteins from many biogenic minerals are implicated in directing the formation of crystal polymorphs and morphologies. We characterize the first extremely acidic proteins purified from biomineralized aragonite. These abalone nacre proteins are two variants of 8.7 and 7.8 kDa designated AP8 (for aragonite proteins of approximately 8 kDa). The AP8 proteins have compositions dominated by Asx (∼35 mol %) and Gly (∼40 mol %) residues, suggesting that their structures have high Ca 2+ -binding capacity and backbone flexibility. The growth of asymmetrically rounded CaCO 3 crystals in the presence of AP8 reveals that both proteins preferentially interact with specific locations on the crystal surface. In contrast, CaCO 3 crystals grown with nacre proteins depleted of AP8 retain the morphology of unmodified calcite rhombohedra. Our observations thus identify sites of protein-mineral interaction and provide evidence to support the long-standing theory that acidic proteins are more effective crystal-modulators than other proteins from the same biomineralized material.

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“…Literature resources provide a wide variety of organic additives employed in the morphosynthesis of CaCO 3 . Among these additives are some organic solvents in the presence of CTAB [8], PVP [9], soluble proteins extracted from nacre [10], PEG, PVA, CTAB, and PAA [11], using of amino acids [12], applying ethanol as a solvent [13], and others.…”

Section: Introductionmentioning

confidence: 99%

Morphosynthesis of CaCO3 at different reaction temperatures and the effects of PDDA, CTAB, and EDTA on the particle morphology and polymorph stability

2007

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Polymorph and morphology of calcium carbonate crystals induced by proteins extracted from mollusk shell

Cited by 105 publications

References 19 publications

Evolution of Nacre: Biochemistry and Proteomics of the Shell Organic Matrix of the Cephalopod Nautilus macromphalus

Evolution of Nacre: Biochemistry and Proteomics of the Shell Organic Matrix of the Cephalopod Nautilus macromphalus

CaCO₃Biomineralization: Acidic 8-kDa Proteins Isolated from Aragonitic Abalone Shell Nacre Can Specifically Modify Calcite Crystal Morphology

Morphosynthesis of CaCO3 at different reaction temperatures and the effects of PDDA, CTAB, and EDTA on the particle morphology and polymorph stability

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Polymorph and morphology of calcium carbonate crystals induced by proteins extracted from mollusk shell

Cited by 105 publications

References 19 publications

Evolution of Nacre: Biochemistry and Proteomics of the Shell Organic Matrix of the Cephalopod Nautilus macromphalus

Evolution of Nacre: Biochemistry and Proteomics of the Shell Organic Matrix of the Cephalopod Nautilus macromphalus

CaCO3Biomineralization: Acidic 8-kDa Proteins Isolated from Aragonitic Abalone Shell Nacre Can Specifically Modify Calcite Crystal Morphology

Morphosynthesis of CaCO3 at different reaction temperatures and the effects of PDDA, CTAB, and EDTA on the particle morphology and polymorph stability

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Product

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CaCO₃Biomineralization: Acidic 8-kDa Proteins Isolated from Aragonitic Abalone Shell Nacre Can Specifically Modify Calcite Crystal Morphology