Polymorphic karyotypes and sex chromosomes in the tufted deer &lt;i&gt;(Elaphodus cephalophus)&lt;/i&gt;: cytogenetic studies and analyses of sex chromosome-linked genes

Cao, Xiangrong; Jiang, Huayun; Zhang, X.

doi:10.1159/000084212

Cited by 14 publications

(7 citation statements)

References 43 publications

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“…The G-band patterns were obtained following a modification of the previous study [9] . Fiveday old slides were treated with 0.25% trypsin in pH 6.8 phosphate buffered saline (without magnesium and calcium) for 1 min, rinsed in normal saline and stained with 10% Giemsa for 5 min.…”

Section: G-bandingmentioning

confidence: 99%

Somatic Chromosomes of the Bornean Sambar Deer and Rusa Deer Interspecific Hybrids

Idris¹

2009

American Journal of Applied Sciences

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Problem statement: Hybridization has potential benefits to the Malaysian farmed deer industries in terms of increased growth rate and increased proportion of muscle and an improved alignment of feed supply and annual energy requirement. Species or subspecies of different chromosome constitution could mate to produce healthy hybrid offspring in a normal ratio of males and females. If any of the hybrid offspring were sterile, the sterile offspring would be the heterogametic offspring. The study investigated the use of chromosome banding method to detect chromosomal variation and to define the chromosome homology and the possibility of the Bornean Sambar deer (Cervus unicolor brookei) and Rusa deer (Cervus timorensis) hybrids to reproduce. Approach: Samples were collected from the Livestock Breeding Station, Sabrang, Keningau, Sabah, East Malaysia. The animals studied consisted of two deer subspecies namely the Bornean Sambar deer, Rusa deer and their hybrids. The karyotypes of the Bornean Sambar deer, Rusa deer and their F1 hybrids have been investigated by solid giemsa staining, G-banding and Ag-NOR banding techniques. Results: Rusa and Bornean Sambar have different chromosome number; 60 and 62 respectively, but share the same fundamental number of chromosome arm, 70. The hybrids have 2n = 61, consisting of 9 metacentric to submetacentric autosomes and 24 pairs of acrocentric autosomes with two acrocentrics and one submetacentric chromosome being unpaired. The morphology of the sex chromosomes in the F1 hybrids was similar to that of the parental species. The Ag-NOR pattern and the conventional Giemsa staining of chromosomes were effective as markers in the characterization of the karyotypes of the parental lines and hybrids because of the presence of active NORs on different chromosomes of different species. G-band, in contrast, showed complete homology in the presence of euchromatic bands and heterochromatin blocks respectively on each chromosome, suggesting that the two species have few genetic differences. Conclusion: In the present study, natural mating between Bornean Sambar deer and Rusa deer were conducted and the number and chromosomal location of the nucleolar organizer regions in their offsprings were analyzed by the silver staining method. Apart from that, the interspecific dissimilarities with regards to chromosome number and morphology are less extensive and the production of chromosomally balanced gametes could be expected.

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Section: G-bandingmentioning

confidence: 99%

Somatic Chromosomes of the Bornean Sambar Deer and Rusa Deer Interspecific Hybrids

Idris¹

2009

American Journal of Applied Sciences

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“…They are endangered animals distributed in Southern and Southwestern China and North Myanmar (Ohtaishi and Gao 1990). Previous reports showed that there is huge chromosomal polymorphism in the tufted deer: 2n ¼ 46/47/48 in female and 2n ¼ 47/48 in male (Shi 1981;Zhang et al 1983;Wang and Quan 1984;Shi et al 1991;Cao et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Identification of complete mitochondrial genome of the tufted deer

et al. 2008

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The tufted deer Elaphodus cephalophus are endangered animals in the world and little is understood about their mitochondrial (mt) genome. In our study, the mt genome of the tufted deer is identified-which is about 16 kb in length and contains 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and a non-coding sequence (control region). The distinguishing feature is that GTG is the start codon of the NADH4L gene and the cyt b gene has a subterminal AAA followed by the stop codon TAG. According to 12 H strand protein-coding genes and phylogenetic analysis, Elaphodus may have a sister relationship with another deer group Muntiacus.

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“…However, additionally to the Mazama genus, there are other reports of sexautosome translocations among Cervidae family, including muntjacs (Muntiacus muntjak and M. crinifrons) and the tufted deer ( Elaphodus cephalophus) [Wurster and Benirschke, 1970;Shi et al, 1991;Yang et al, 1995;Cao et al, 2005]. This type of chromosomal rearrangement is also described in bats, rodents, bovids, and primates [Ratomponirina et al, 1986;Dobigny et al, 2004;Solari and Rahn, 2005;Noronha et al, 2010;Veyrunes et al, 2014;Vozdova et al, 2016].…”

Section: Discussionmentioning

confidence: 91%

Intrapopulation Chromosomal Polymorphism in <b><i>Mazama gouazoubira</i></b> (Cetartiodactyla; Cervidae): The Emergence of a New Species?

Valeri

Tomazella

Duarte³

2018

Cytogenet Genome Res

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Mazama gouazoubira is a small deer species widely distributed in South America. Previous studies have shown that this species presents intraspecific chromosomal polymorphisms, which could affect fertility due to the effects of chromosomal rearrangements on gamete formation. Important aspects regarding the karyotype evolution of this species and the genus remain undefined due to the lack of information concerning the causes of this chromosomal variation. Nineteen individuals belonging to the Mazama gouazoubira population located in the Pantanal were cytogenetically evaluated. Among the individuals analyzed, 9 had B chromosomes and 5 carried a heterozygous centric fusion (2n = 69 and FN = 70). In 3 individuals, the fusion occurred between chromosomes X and 16, in 1 individual between chromosomes 7 and 21, and in another individual between chromosomes 4 and 16. These striking polymorphisms could be explained by several hypotheses. One is that the chromosome rearrangements in this species are recent and not fixed in the population yet, and another hypothesis is that they represent a balanced polymorphism and that heterozygotes have an adaptive advantage. On the other hand, these polymorphisms may negatively influence fertility and raise questions about sustainability or reproductive isolation of the population.

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Polymorphic karyotypes and sex chromosomes in the tufted deer <i>(Elaphodus cephalophus)</i>: cytogenetic studies and analyses of sex chromosome-linked genes

Cited by 14 publications

References 43 publications

Somatic Chromosomes of the Bornean Sambar Deer and Rusa Deer Interspecific Hybrids

Somatic Chromosomes of the Bornean Sambar Deer and Rusa Deer Interspecific Hybrids

Identification of complete mitochondrial genome of the tufted deer

Intrapopulation Chromosomal Polymorphism in <b><i>Mazama gouazoubira</i></b> (Cetartiodactyla; Cervidae): The Emergence of a New Species?

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