Polymorphic microsatellite DNA markers for the Amazonian catfish Pseudoplatystoma punctifer (Siluriformes: Pimelodidae)

Saulo-Machado, A. C.; Formiga, Kyara Martins; Ortiz, Mauro Freitas; Sousa, Adna Cristina Barbosa de; Alves-Gomes, José Antônio; Batista, Jacqueline S.

doi:10.1007/s12686-010-9349-4

Cited by 15 publications

(9 citation statements)

References 11 publications

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“…tigrinum corroborate those observed in other congeners, such as P. punctifer (SAULO-MACHADO et al 2010), P. reticulatum (PRADO et al 2014 and P. corruscans (REVALDAVES et al 2005) (Table 3).…”

Section: Resultssupporting

confidence: 84%

First Polymorphic Microsatellite Markers for the Migratory Goliath Catfish Pseudoplatystoma Tigrinum (Siluriformes: Pimelodidae), a Commercially Important Resource in the Amazon Basin / Primeiros Marcadores Microssatélites Polimórficos Para O Bagre Migratório Pseudoplatystoma Tigrinum (Siluriformes: Pimelodidae), Um Recurso Comercialmente Importante Na Bacia Amazônica

Oliveira

Guimarães-Marques²,

Carvalho

et al. 2020

BJD

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The caparari (Pseudoplatystoma tigrinum) is a widely distributed species in the Amazon Basin. It has a high market value and it is commonly consumed by the local population. In order to protect the species from possible overfishing, there is a need for a better understanding of the population diversity, since knowledge regarding its biology is scarce, which makes it difficult to monitor. Seven microsatellite loci (simple sequence repeats-SSR) for the species were isolated and characterized in 46 individuals which were sampled in four locations from the Madeira River, in the Brazilian Amazon. The number of alleles per locus ranged from three to eleven. The observed and expected heterozygosity ranged from 0,326 to 0,705 and 0,322 to 0,758, respectively. No linkage disequilibrium between pairs of loci was detected. The seven microsatellite loci were additionally used for inter-specific amplification in other four species of Pseudoplatystoma. Therefore, this study contributes to the first molecular species-specific SSR markers, which can be used as a new tool for estimating the genetic variability of P. tigrinum and has potential for application in population-related studies.

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Section: Resultssupporting

confidence: 84%

First Polymorphic Microsatellite Markers for the Migratory Goliath Catfish Pseudoplatystoma Tigrinum (Siluriformes: Pimelodidae), a Commercially Important Resource in the Amazon Basin / Primeiros Marcadores Microssatélites Polimórficos Para O Bagre Migratório Pseudoplatystoma Tigrinum (Siluriformes: Pimelodidae), Um Recurso Comercialmente Importante Na Bacia Amazônica

Oliveira

Guimarães-Marques²,

Carvalho

et al. 2020

BJD

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“…This value was within the range usually observed in other related species, such as P. corruscans in the Amazon basin and P. reticulatum in the Paraguay River Saulo-Machado et al, 2011; see also Carvalho et al, 2012 for a comparison of P. corruscans between Parana and São Francisco basins). However, in this study, F ST values tended to be slightly correlated with geographical distances according to Mantel test.…”

Section: Discussion Population Differentiation In P Punctifersupporting

confidence: 82%

“…Some previous studies investigated population genetic patterns in species of the genus Pseudoplatystoma, including Revaldaves et al (2005), who developed microsatellite primers for P. corruscans and tested its transferability to other species, including Pseudoplatystoma fasciatum (which is closely related, or even completely overlapped, with P. punctifer (see Buitrago-Suarez and Burr, 2007;Torrico et al, 2009;Carvalho-Costa et al, 2011). Saulo-Machado et al (2011) recently developed specific microsatellite markers for P. punctifer and described some genetic parameters within a local population. Pereira et al (2009) recently showed that a significant population differentiation occurred among local populations of P. corruscans, with an F ST around 8% and a significant spatial pattern in genetic differentiation according to Mantel test, by using 6 local populations (and interpreted such patterns as a consequence of homing behavior).…”

Section: For Reviews)mentioning

confidence: 99%

Geographical genetics of Pseudoplatystoma punctifer (Castelnau, 1855) (Siluriformes, Pimelodidae) in the Amazon Basin

Telles¹,

Collevatti²,

Braga³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Geographical genetics allows the evaluation of evolutionary processes underlying genetic variation within and among local populations and forms the basis for establishing more effective strategies for biodiversity conservation at the population level. In this study, we used explicit spatial analyses to investigate molecular genetic variation (estimated using 7 microsatellite markers) of Pseudoplatystoma punctifer, by using samples obtained from 15 localities along the Madeira River and Solimões, Amazon Basin. A high genetic diversity was observed associated with a relatively low F ST (0.057; P < 0.001), but pairwise F ST values ranged from zero up to 0.21 when some pairs of populations were compared. These F ST values Geographical genetics of Pseudoplatystoma punctifer have a relatively low correlation with geographic distances (r = 0.343; P = 0.074 by Mantel test), but a Mantel correlogram revealed that close populations (up to 80 km) tended to be more similar than expected by chance (r = 0.360; P = 0.015). The correlogram also showed a exponential-like decrease of genetic similarity with distance, with a patch-size of around 200 km, compatible with isolation-by-distance and analogous processes related to local constraints of dispersal and spatially structured levels of gene flow. The pattern revealed herein has important implications for establishing strategies to maintain genetic diversity in the species, especially considering the threats due to human impacts caused by building large dams in this river system.

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“…A total of six SSR markers were evaluated, four originally developed for Brachyplatystoma rousseauxi (Batista, Farias, Formiga-Aquino, Sousa, & Alves-Gomes, 2010) and two for Pseudoplatystoma punctifer (Saulo-Machado et al, 2011). Table 1 shows sequence information, annealing temperature, and GenBank accession number.…”

Section: Amplification Of Microsatellite Primersmentioning

confidence: 99%

Obtaining microsatellite markers for Pseudoplatystoma reticulatum using heterologous primers

Penafort

Mantovani

Granzoto

et al. 2021

Semina: Ciênc. Agrár.

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Studies on genetic composition in fish populations contribute to conservationist practices and inbreeding control in fish stocks. To this end, molecular tools such as microsatellite markers (SSRs) are often used, but they are expensive and time-consuming to develop. A species-specific heterologous marker emerges as an alternative, which can be used in taxonomically related species in a fast way. Our goal was to test SSRs markers of Brachyplatystoma rousseauxi and Pseudoplatystoma punctifer in P. reticulatum in an unprecedented way. For this purpose, DNA was extracted from fragments of the caudal fin of 222 P. reticulatum adults, using a NaCl-based method. Then, DNA samples were amplified by Polymerase Chain Reaction (PCR) using six markers, four from B. rousseauxi (BR38, 47, 51, and 61) and two from P. punctifer (PPU13 and PPU15). Two primers showed non-specific amplification and were disregarded (BR38 and PPU13). In the remaining four primers, the number of alleles per locus varied between two (BR47) to sixteen (BR51), and the average size of alleles was between 142 and 400 bp. Mean effective number of alleles per locus ranged from 10,650 (BR51) to 1,784 (BR47), with null or low-frequency alleles in all studied loci. Observed heterozygosity ranged from 0.299 (BR47) to 0.640 (BR51) and was always lower than the expected heterozygosity. Hardy-Weinberg balance was significant (p < 0.05) in all loci, and inbreeding coefficient (FIS) was always positive. Polymorphic Information Content (PIC) confirmed the efficiency of the markers since they had moderate (BR47) to high levels of information (BR51, BR61, and PPU15). Transferability test showed that the heterologous microsatellite molecular markers, originally for B. rousseauxi and P. punctifer, were efficient in P. reticulatum, producing three primers with high information content. Therefore, these markers can be safely used in future population studies of this species.

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Polymorphic microsatellite DNA markers for the Amazonian catfish Pseudoplatystoma punctifer (Siluriformes: Pimelodidae)

Cited by 15 publications

References 11 publications

Geographical genetics of Pseudoplatystoma punctifer (Castelnau, 1855) (Siluriformes, Pimelodidae) in the Amazon Basin

Obtaining microsatellite markers for Pseudoplatystoma reticulatum using heterologous primers

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