Polymorphic microsatellite markers for Philippine tarsiers (Tarsius syrichta)

Abstract

This study reports the isolation and characterization of eight microsatellite markers for the study of Philippine tarsiers (Tarsius syrichta), small primates endemic to this Southeast Asian archipelago. The markers were used to screen 14 Tarsius syrichta for allelic diversity. This suite of highly polymorphic microsatellites provides the first chance to genetically study parentage and dispersal patterns in Philippine tarsiers.

Conservation Genetics of the Philippine Tarsier: Cryptic Genetic Variation Restructures Conservation Priorities for an Island Archipelago Primate

“…Each locality was represented by 1–27 individuals (mean = 3.88). DNA was extracted using QIAGEN DNeasy extraction kits and we used nine microsatellite makers previously developed for Tarsius syrichta : T5, T6, T22, T34, T35, T43, T69 [26] and Tarsius spp. : T42, T50 [27] .…”

“…Primers T42, T43 and T45 were fluorescently labeled with 6-FAM by Integrated DNA Technologies (Coralville, Iowa) for PCR. Primer annealing temperatures for these three loci were as follows: T42 at 53°C [27] , T43 at 54°C and T45 at 57°C [26] . Subsequently, 2.1 µl of the PCR product was added to 9 µl of formimide and .15 µl was electrophoresed with the LIZ-500 size standard and analyzed on a 3730 Genetic Analyzer (Applied Biosystems).…”