Polymorphic microsatellite markers for the Florida scrub lizard (<i>Sceloporus woodi</i>)

Ernst, Joel A.; Branch, Lyn C.; Clark, Ann; Hokit, D. Grant

doi:10.1111/j.1471-8286.2004.00654.x

Cited by 12 publications

(8 citation statements)

References 5 publications

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“…We used a total of seven microsatellites developed for scrub lizards: SW614-A1, SW614-A4, SW614-A6, SW614-A7, SW614-B6, SW614-B10, and SW614-B12. Detailed laboratory protocols, repeat motifs and primer sets are presented in Ernst et al (2004). All PCR products were run on a capillary sequencer MegaBace TM 1000 (Amersham Biosciences/GE Healthcare) using GeneScan TM -500 [ROX] TM (Applied Biosystem) as size standard.…”

Section: Molecular Methodsmentioning

confidence: 99%

Ecological metrics predict connectivity better than geographic distance

et al. 2009

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We use microsatellite loci to examine genetic structure of the Florida scrub lizard (Sceloporus woodi) and test for the effects of landscape variables at the scale of neighboring patches. We evaluate ecological metrics of connectivity with genetics data, which to our knowledge is the first application of these particular metrics to landscape-level genetics studies in Florida scrub. Florida scrub is a highly threatened ecosystem in which habitat patches are remnants of a previously widespread xeric landscape. Analysis of mitochondrial DNA (mtDNA) has shown that landscape structure influenced the evolutionary history of the Florida scrub lizard (S. woodi) across its range. Our results concur with these mtDNA studies in documenting divergence between xeric ridge systems and also demonstrate divergence at very local scales. Both least-cost distance and pairwise isolation (a metric used in ecological studies that includes patch size, quality and a modified isolation index) were better predictors of genetic distance than Euclidean distance, indicating that mesic and hydric habitat influence spatial patterns in genetic variation. Our results support the need for focusing on spatial distribution of scrub habitat at the scale of neighboring patches, as well as regionally, in conservation management and restoration. Also, our study points to the value of integrating landscape ecology metrics into landscape genetics.

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Section: Molecular Methodsmentioning

confidence: 99%

Ecological metrics predict connectivity better than geographic distance

et al. 2009

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“…We investigated morphological and agonistic behavioural traits exhibited by hybrids of Sceloporus lizards ( Sceloporus undulatus Bosc and Daudin subspecies undulatus and Sceloporus woodi Stejneger) from an established, viable hybrid zone (Jackson 1973). Preliminary genetic analyses, using six previously published microsatellites for S. woodi (Ernst et al. 2004), indicated 95% of 44 individuals from the hybrid zone were consistent with hybrid lineage and suggested a hybrid swarm that included high levels of backcrossing to S. undulatus .…”

Section: Introductionmentioning

confidence: 94%

Transgressive aggression in Sceloporus hybrids confers fitness through advantages in male agonistic encounters

Robbins¹,

Pruitt

Straub

et al. 2009

Journal of Animal Ecology

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Summary1. We investigated agonistic behaviour and associated characteristics of Sceloporus woodi (Florida scrub lizard), Sceloporus undulatus (Eastern fence lizard) and their hybrids using staged territorial encounters. 2. These Sceloporus hybrids exhibit transgressive aggression and transgressive head-girth relative to the parental species and the transgressive aggression was specifically associated with an advantage in agonistic encounters. Our results suggest a hybrid advantage in natural habitats when defending and invading territories against either parental species. 3. We further analysed general advantages in agonistic encounters across the entire three-group system to elucidate characteristics that may be advantageous under specific circumstances. Individuals with larger body size (SVL) and greater aggression had an overall advantage in agonistic encounters; however, smaller individuals could win when slightly more aggressive and fatter, and less aggressive individuals could win when slightly larger, especially with greater head-girth. 4. The extreme hybrid phenotypes likely occurred through transgressive segregation, which has been implicated as a process through which homoploid, hybrid speciation can occur. Some form of ecological divergence is necessary, however, to impede parental gene flow. Our data suggest that ecological divergence could manifest in territorial species through transgressive aggression.

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“…We used an enrichment procedure based on Ernst et al (2004). Genomic DNA was extracted using the DNeasy Mini Kit (Qiagen, Valencia, CA) and digested with Sau 3AI.…”

Section: Methods and Resultsmentioning

confidence: 99%

Characterization of polymorphic microsatellite loci in Haageocereus (Trichocereeae, Cactaceae)

Arakaki

Soltis

et al. 2010

American J of Botany

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Haageocereus (Cactaceae), a genus of 20 accepted species, is restricted to the western slopes of the Andes and is one of the most taxonomically complex genera in Cactaceae. Haageocereus is considered vulnerable to extinction threats, and seven species have been classifi ed as in danger of disappearance throughout all or a signifi cant portion of their ranges ( Ostolaza, 1996 ). Haageocereus tenuis F. Ritter (2 N = 3 x = 33) consists of a single population restricted to two square kilometers close to the city of Lima; H. repens Rauh & Backeb. (2 N = 2 x = 22) formerly comprised a single natural population, with about 10% of all known individuals translocated to botanical institutions before its entire range was converted to agriculture. Both H. acranthus (Vaup.) Backeb. (2 N = 4 x = 44) and H. pseudomelanostele (Werdermann & Backeb.) Backeb. (2 N = 2 x = 22) consist of several large populations but mostly restricted to the Department of Lima.Our goal was to develop microsatellite loci to examine the amount of genetic diversity in rare ( H. tenuis and H. repens ) and widespread ( H. pseudomelanostele and H. acranthus ) species of Haageocereus and provide crucial information for future conservation plans. METHODS AND RESULTSWe used an enrichment procedure based on Ernst et al. (2004) . Genomic DNA was extracted using the DNeasy Mini Kit (Qiagen, Valencia, CA) and digested with Sau 3AI. DNA was purifi ed and fractioned using Chroma Spin columns (Clontech Laboratories, Mountain View, CA), which removed fragments smaller than 400 base pairs. The remaining DNA was ligated to Sau 3AI linkers and amplifi ed by PCR. The fragment library was enriched for (CA) n repeats by hybridizing DNA fragments to a biotinylated nucleic acid probe. Probe-target fragments were captured using a VECTREX Avidin D matrix (Vector Laboratories, Burlingame, CA). Eluted fragments were amplifi ed by PCR, ligated into a TOPO TA pCR vector and transformed into One Shot E. coli competent cells (Invitrogen, Carlsbad, CA). Colonies were screened by binding them to nitrocellulose membranes (Osmonics, Minnetonka, MN) which were hybridized with labeled (CA) n probe; positive colonies were detected by Lumi-Phos 480 (Lifecodes TM , Stamford, CT). The selected colonies were grown overnight in Luria broth, plasmids were purifi ed using QIAprep Spin Miniprep Kit (Qiagen) and screened once more by dot-blotting serial dilutions on nylon membranes. Plasmids were hybridized to a (CA) n probe, and those containing the target repeats were sequenced on a CEQ 8000 capillary sequencer (Beckman-Coulter, Brea, CA).Primers were developed using Primer 3 (Rozen and Skaletsky, 2000 ). An M13 tail (5 ′ -CACGACGTTGTAAAAC-3 ′ ) was added to each forward primer and labeled with D4 (Beckman-Coulter). PCR amplifi cation procedures were optimized for each primer pair following Speranza and Malosetti (2007) . PCR amplifi cations were performed in 10-μ L total volumes containing 0.2 unit of NEB Taq polymerase (New England Biolabs, Ipswich, MA), 1.5 mM MgCl 2 , 0.15 μ M of the rever...

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Polymorphic microsatellite markers for the Florida scrub lizard (Sceloporus woodi)

Cited by 12 publications

References 5 publications

Ecological metrics predict connectivity better than geographic distance

Ecological metrics predict connectivity better than geographic distance

Transgressive aggression in Sceloporus hybrids confers fitness through advantages in male agonistic encounters

Characterization of polymorphic microsatellite loci in Haageocereus (Trichocereeae, Cactaceae)

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