Polymorphic stages of the fresh water blue-green alga, Gomphosphaeria aponina

Dwivedi, Vinay; Tandon, R. N.; Tiwari, G. L.

doi:10.4490/algae.2010.25.3.115

Cited by 2 publications

(1 citation statement)

References 2 publications

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“…The benchtop FlowCAM® VS‐IV (Fluid Imaging Technologies, Yarmouth, Maine, U.S.A.) was equipped with VisualSpreadsheet ® Version 3.8.2 software and auto‐focused for ×20, ×10, ×4, and ×2 objectives, respectively. Taxonomic references included Desikachary (), Dwivedi et al (), Findlay and Kling (), Komárek and Anagnostidis (, , ), Komárek (), Komárek and Mares (), Komárek and Zapomelova (, ), McGregor et al (), Nguyen et al (), Prescott (), Rott (), and Willame et al (). All taxonomic analyses using both the FlowCam and light microscope were performed by the same taxonomist (M.D.…”

Section: Instrumentationmentioning

confidence: 99%

High‐resolution imaging particle analysis of freshwater cyanobacterial blooms

Graham

Cook

Graydon

et al. 2018

Limnology & Ocean Methods

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Effective assessment of the health risk of cyanobacterial blooms requires an early warning system, which enables rapid detection of species of concern and determination of whether their cell concentrations exceed advisory guidelines. Advanced digital flow cytometry using FlowCam® (Fluid Imaging Technologies) in combination with light microscopy is a solid prospect for tracking cyanobacterial communities in a timely manner. However, implementation of such a method poses several challenges for the user. We first address sample preparation, instrumentation, taxonomic enumeration, and trouble‐shooting to facilitate high throughput of analyses of water samples for total cyanobacterial cell counts and their species composition. Preservation and initial screening of samples using light microscopy to estimate community size structure are endorsed to insure their archival quality and avoid clogging of the flow cell. We show that the highest magnification (×20 objective) is needed to achieve representative total and species‐specific cell enumerations. We also report that total cyanobacterial cell counts for samples analyzed using FlowCam vs. inverted light microscopy show significant positive correlation, as do those for preserved vs. live samples. Quantification of community composition using FlowCam vs. light microscopy also shows strong concordance. Although our FlowCam method performs well in the context of the World Health Organization advisory threshold of a total cyanobacterial count of 100,000 cells mL−1, it remains a work in progress in terms of reliably automated species‐level identifications.

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Section: Instrumentationmentioning

confidence: 99%

High‐resolution imaging particle analysis of freshwater cyanobacterial blooms

Graham

Cook

Graydon

et al. 2018

Limnology & Ocean Methods

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Genomic Mysteries of Giant Bacteria: Insights and Implications

Ionescu,

Volland,

Contarini

et al. 2023

Genome Biology and Evolution

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Bacteria and Archaea are traditionally regarded as organisms with a simple morphology constrained to a size of 2-3 micrometers. Nevertheless, the history of microbial research is rich in description of giant bacteria exceeding tens, and even hundreds of micrometers in length or diameter already from its early days, e.g. Beggiatoa spp, to the present, e.g. Ca. Thiomargarita magnifica. While some of these giants are still being studied, some were lost to science, with merely drawing and photomicrographs as evidence for their existence. The physiology and biogeochemical role of giant bacteria has been studied, with a large focus on those involved in the sulfur cycle. With the onset of the genomic era, no special emphasis has been given to this group, in an attempt to gain a novel, evolutionary and molecular, understanding of the phenomenon of bacterial gigantism. The few existing genomic studies reveal a mysterious world of hyper-polyploid bacteria with hundreds to hundreds-of-thousands of chromosomes which are in some cases identical and in others, extremely different. These studies on giant bacteria reveal novel organelles, cellular compartmentalization, and novel mechanisms to combat the accumulation of deleterious mutation in polyploid bacteria. In this perspective paper, we provide a brief overview of what is known on the genomics of giant bacteria and build on that to highlight a few burning questions that await to be addressed.

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Polymorphic stages of the fresh water blue-green alga, Gomphosphaeria aponina

Cited by 2 publications

References 2 publications

High‐resolution imaging particle analysis of freshwater cyanobacterial blooms

High‐resolution imaging particle analysis of freshwater cyanobacterial blooms

Genomic Mysteries of Giant Bacteria: Insights and Implications

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