Polymorphism analysis of the hsp70 stress gene in Broiler chickens (Gallus gallus) of different breeds

Mazzi, Carmen Maria; Ferro, Jesus Aparecido; Ferro, Maria Inês Tiraboschi; Savino, Vicente José Maria; Coelho, Antônio Augusto Domingos; Macari, Marcos

doi:10.1590/s1415-47572003000300010

Cited by 40 publications

(44 citation statements)

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“…The relationship of HSP70 with the survival ability of cells affected by heat stress was extensively investigated by Yu & Bao (2008). Mazzi et al (2003); recently reported a nucleotide polymorphism in the coding region of HSP70 in chickens. Duangduen et al (2007) found that this mutation, although silent, may possibly be used as a heat tolerance marker in indigenous and commercial broiler strains.…”

Section: Hsp70 Genotypes and Heat Tolerance Of Commercial And Native mentioning

confidence: 99%

“…DNA quality and concentration were determined by UV spectroscopy (NanoVue, GE Healthcare, UK).The DNA was diluted to 50 mg/mL for use as a working solution and stored at −20 o C before analyses. The HSP70 gene was amplified by PCR, as explained in Mazzi et al (2003). Primers HSP70-F (5'-AAC CGC ACC ACA CCC AGC TAT G -3') and HSP70-R (5'-CTG GGA GTC GTT GAA GTA AGC G -3') were used.…”

Section: Data Collectionmentioning

confidence: 99%

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Hsp70 Genotypes and Heat Tolerance of Commercial and Native Chickens Reared in Hot and Humid Conditions

Duangjinda

Tunim

Duangdaen

et al. 2017

Rev. Bras. Cienc. Avic.

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Heat tolerance in poultry production was obtained attention due to the need for genetic lines that can withstand climate changes. This study aimed at investigating heat tolerance in commercial and native broiler genetics, as well as the physiological and growth performance responses of HSP70 genotypes submitted to heat stress. In Experiment I, heterophil:lymphocyte (H:L) ratio, as an indicator of heat tolerance, was compared between commercial broilers (n = 100) and Thai native chickens (n = 100). Growing chickens (with similar initial weight) of each genetic strain were randomly divided into two groups: 1) thermoneutral environment (26 o C ± 2 o C) and 2) heat stress (36 o C ± 2 o C). The results showed that native chickens originating from a tropical environment presented lower H:L ratio and mortality rate compared with commercial broilers. In Experiment II, HSP70 genotypes were compared. PCR-RFLP was applied to identify the genotypes (C1C1, n = 38; C1C2, n = 38; and C2C2, n = 28). Ten-week-old chickens of each genotype were evaluated in the same environments described in Experiment I. Heat-stress indicators -respiratory rate (RR), cloacal temperature (CT), packed cell volume (PCV), and average daily gain (ADG) -were measured for three weeks. The significant difference in PCV indicated that C2C2 chickens were less tolerant to heat stress compared to other genotypes. The RR, CT, and ADG were not significantly different among all genotypes. Since the C2C2 genotype was shown to be sensitive to heat stress, C1C1 and C1C2 could be used as markers for heat-tolerant genetic strains of Thai indigenous chickens and hybrid commercial lines.

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Section: Hsp70 Genotypes and Heat Tolerance Of Commercial And Native mentioning

confidence: 99%

Section: Data Collectionmentioning

confidence: 99%

Hsp70 Genotypes and Heat Tolerance of Commercial and Native Chickens Reared in Hot and Humid Conditions

Duangjinda

Tunim

Duangdaen

et al. 2017

Rev. Bras. Cienc. Avic.

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“…Zhang et al (2002) also detected polymorphism in regulatory and coding regions of HSP70 in chickens with various heat tolerance capabilities using polymerase chain reaction (PCR) single-strand conformation polymorphism analysis and sequencing. Mazzi et al (2003) analyzed the promoter region and the beginning of the coding region of HSP70 in chickens with various heat tolerance capabilities and found 2 single nucleotide polymorphisms (SNPs) -A+258G and C+276G -both of which were silent mutations. Zhen et al (2006) tested the chicken HSP70 mRNA expression of various genotypes at polymorphism sites C+276G and A+258G in liver and leg muscle tissue using real-time quantitative PCR (qPCR).…”

Section: Introductionmentioning

confidence: 99%

Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress

Gan

Zhang²,

et al. 2013

Genet. Mol. Res.

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ABSTRACT. DNA methylation plays a central role in gene expression. In this study, we detected the promoter methylation pattern of the chicken heat shock protein 70 (HSP70) gene and its association with messenger RNA (mRNA) expression before and after heat shock. The results showed that mRNA expression increased in response to heat stress and peaked at 3 h before dropping. Hypomethylation of the HSP70 promoter occurred in all of the groups studied, but the difference between groups within tissue type was not significant. The DNA methylation level of the control and the 6-h treatment groups was slightly higher than that of the 3-h treatment group in brain tissue and leg muscle. Correlation analysis between mRNA expression and DNA methylation of HSP70 showed that DNA methylation was negatively associated with mRNA expression in leg muscle (P = 0.0124), indicating that DNA methylation may be negatively associated with the expression of HSP70, although the difference was not significant. We concluded that the expression of HSP70 is heat inducible and tissue dependent and that heat induction may correlate with DNA methylation pattern in the HSP70 promoter, whereas tissue dependence is unrelated to DNA methylation pattern.

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“…60 A strong relationship was also established between chicken and HSP 70 genotypes during heat resistance and several studies revealed that there are some polymorphism sites that can be effectively used to identify the heat tolerant trait in chicken respectively. [61][62][63] …”

Section: Types Of Hsps Studied In Livestock During Hsmentioning

confidence: 99%

Role of Heat Shock Proteins in Livestock Adaptation to Heat Stress

PR¹

2017

JDVAR

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The present review is an attempt to signify the importance of heat shock proteins in livestock adaptation during heat stress. The cellular and molecular responses in livestock are very crucial as it may lead to identification of confirmatory biomarker for heat stress in livestock. Thermo-tolerant gene expression and elevated heat shock protein (HSP) levels are observed to be the ultimate response through which the cell survives the heat stress. The HSPs have chaperonic activity ensuring the folding, unfolding and refolding of stress-denatured proteins. The components of heat shock response include heat shock factors (HSFs), heat shock element (HSE) and HSP. The cellular response to heat stress in mammalian organisms is controlled at the transcription level and it is mediated by a family of HSF which are regulated by the corresponding HSF genes. The activated HSFs bind with the HSE in the promoter region of HSP genes culminating in enhanced transcription of HSP mRNA. The HSP70, HSP90 and HSP27 are the predominant HSPs having protective role during heat stress in farm animals. Among these HSPs studied, HSP70 was identified to be the ideal biological marker for quantifying heat stress in animals.Keywords: heat stress, heat shock factors, heat shock response, hsp70, livestock Journal of Dairy, Veterinary & Animal Research Review Article Open AccessRole of heat shock proteins in livestock adaptation to heat stress the latter as it is harder to find an environment with a single variable changing. However, both these mechanisms are phenotypic responses as it is induced by the environment and the response goes once the stress is removed. It is usually produced to improve the fitness of the animal to the environment. Animals develop specific adaptive mechanisms if the environment stressors are present for a prolonged period. The mechanisms of adaptation include morphological, behavioural, biochemical and physiological changes. Biochemical adaptation to thermal stress involves changes in proteins, membrane lipids and metabolic rate.7 Genetic markers are of prior importance in the recent revolutionary developments in the field of molecular technology because of its viability as the biochemical adaptation can be utilized in livestock breeding programs compared to the behavioral, morphological or physiological responses to stress which is of less relevance. Heat shock protein is an important biomarker produced as cellular and tissue defense mechanism whose expression is markedly increased during heat shock. 8 The present review is an attempt to signify the importance of heat shock proteins in livestock adaptation during HS. General cellular responses to heat stressCellular exposure to thermal stress induces a number of anomalies in the functioning of cells which alters the biological molecules, disturbs cell functions, modulates metabolic reactions, induces oxidative cell damage and activates both apoptosis and necrosis pathways, ultimately leading to cell survival, acclimation or cell death depending on the time and...

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Polymorphism analysis of the hsp70 stress gene in Broiler chickens (Gallus gallus) of different breeds

Cited by 40 publications

References 18 publications

Hsp70 Genotypes and Heat Tolerance of Commercial and Native Chickens Reared in Hot and Humid Conditions

Hsp70 Genotypes and Heat Tolerance of Commercial and Native Chickens Reared in Hot and Humid Conditions

Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress

Role of Heat Shock Proteins in Livestock Adaptation to Heat Stress

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