Polymorphism and expression of insulin-like growth factor 1 (IGF1) gene and its association with growth traits in chicken

Abstract

1. The objectives of the study were to detect polymorphism in the coding region of the IGF1 gene, explore the expression profile and estimate association with growth traits in indigenous and exotic chickens. 2. A total of 12 haplotypes were found in Cornish, control layer and Aseel breeds of chicken in which the h1 haplotype was most frequent. 3. Nucleotide substitutions among haplotypes were found at 21 positions in the IGF1 gene in which 4 substitutions resulted in non-synonymous mutations in the receptor binding domain of the IGF1 protein. 4. The haplogroup showed a significant effect on body weight at 24 and 42 d of age in the control layer line, body weight at 42 d and daily weight gain between 29 and 42 d in the control broiler line, daily weight gain between 29 and 42 d in Cornish, and body weights at 42 d as well as daily weight gain between 29 and 42 d in Aseel birds. 5. IGF1 expression varied among the breeds during embryonic and post-hatch periods. The expression among the haplogroups varied in different chicken tissues. The effect of haplogroup on myofibre number in pectoral muscle was non-significant, although there was significant variation in numbers between d 1 and d 42, and between broiler and layer lines. 6. It was concluded that the coding region of the IGF1 gene was polymorphic, expressed differentially during the pre-hatch and post-hatch periods, and haplogroups showed significant association with growth traits in chicken.

Polymorphism analysis and expression patterns of the <i>IGF1</i> gene in the Shitou goose

“…The regulation of IGF1 in animal growth and development has established a relationship between polymorphism and growth performance. Bian et al (2008) identified three SNPs (c.−366A>C, c.528G>A and c. * 1024C>T) that constructed haplotypes in the chicken IGF1 gene. Haplotypes based on three polymorphisms were associated with body weight traits.…”

Polymorphism of insulin-like growth factor 1 gene and its relationship with reproductive performances and milk yield in Sarda dairy sheep

“…Particularly in cattle, a SNPs in the promoter region is linked with greater weight to calf weaning ( de la Rosa Reyna et al., 2010 ; Ge, Davis, Hines, Irvin & Simmen, 2001 ). In chickens and pigs some polymorphisms in the coding region are associated with growth traits ( Bhattacharya et al., 2015 ; Niu et al., 2013 ). In cashmere goats, some SNPs are linked to the wool growth ( Wu-Jun et al., 2010 ).…”

Associations Between IGF1, IGFBP2 and TGFß3 Genes Polymorphisms and Growth Performance of Broiler Chicken Lines

“…The SNPs within the chicken IGF1 promoter were reported by numerous previous studies [54,[86][87][88][89][90][91][92]] but according to the author's best knowledge, no research work on gene constitution of IGF1 SNP in the Hubbard F15 and Cobb E lines has been reported until now.…”