Polymorphism characterization of five canine microsatellites

Mariat, Denis; Kessler, Jean-Louis; Vaiman, Daniel; Panthier, J J

doi:10.1111/j.1365-2052.1996.tb00514.x

Cited by 12 publications

(5 citation statements)

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“…Allelic hypervariability, conservation of the flanking sequence, the co-dominant mode of inheritance, and the abundance of microsatellites, coupled with advances in PCR technology render the molecules a useful marker for closely related species or population genetic studies (Huang et al 1998;Weber and May, 1989;Woodruff, 1993). Due to these advantages, microsatellite DNA has been employed in a variety of organisms (Dallas et al, 1995;Edwards et al 1991;Mariat et al, 1996;Morgante and Olivieri 1993;Pope et al, 1996;Romero et al 2003;Viard et al, 1996). In the silkworm, abundant microsatellite primers have been identified and published by several authors (e.g., Dharma Prasad et al, 2005;Muwang et al, 2005), but strain discrimination has never been the principal objective of marker development efforts.…”

Section: Introductionmentioning

confidence: 94%

Microsatellite analysis of the silkworm strains (Bombyx mori): high variability and potential markers for strain identification

et al. 2010

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The silkworm (Bombyx mori) is of great economic value from an industrial perspective. Casual discrimination and accumulation of genetic information from a diverse variety of silkworms are essential for practical utilization and long-term conservation. In this study, a total of 54 silkworm strains preserved in Korea were typed for nine polymorphic microsatellite loci. We determined per-locus numbers of alleles ranging from 3 to 17 with an average value of 7.5, per-locus observed heterozygosity ranging from 0.04 to 0.98, and per-locus polymorphic information content (PIC) ranging from 0.06 to 0.86, thereby indicating that some of these loci are profoundly variable. Phylogenetic analysis using the nine concatenated microsatellite loci showed no clustering on the basis of known strain characteristics and origin. A total of 17 strain-specific apomorphic alleles, which discriminate 14 among 54 silkworm strains, were obtained from eight loci. These strain-specific alleles can, therefore, be casually utilized to discriminate between applicable strains, without any further typing of other loci. Furthermore, a substantial number of homozygote strains, represented by 24 among 67 alleles in nine loci, were also detected. These results collectively implicate silkworm microsatellite DNA as useful and potentially important molecular markers for the eventual discrimination of silkworm strains, hundreds of which are currently preserved in Korea.

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Section: Introductionmentioning

confidence: 94%

Microsatellite analysis of the silkworm strains (Bombyx mori): high variability and potential markers for strain identification

et al. 2010

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“…Allelic hypervariability, conservation in flanking sequence, co-dominant mode of inheritance and abundance of microsatellites coupled with the advances in PCR technology made the molecules a useful marker for closely related species or population genetic studies (Huang et al, 1998;Weber and May, 1989;Woodruff, 1993). Due by these merits microsatellite DNA has been used in many organisms for a diverse purpose (Edwards et al, 1991;Morgante and Olivieri, 1993;Dallas et al, 1995;Viard et al, 1996;Mariat et al, 1996;Pope et al, 1996;Romero et al, 2003). In the silkworm, abundant microsatellite primers have been published by several authors (e.g., Dharma Prasad et al, 2005), but only some of the Chinese strains kept in Korea were genotyped using microsatellite DNA (Kim et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Microsatellite Analysis of the Silkworm Strains (Bombyx mori) Originated from China

Kim¹,

Kang²,

Ryu³

et al. 2012

International Journal of Industrial Entomology

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A total of 85 Chinese-origin silkworm strains preserved in Korea were genotyped for eight polymorphic microsatellite loci. We obtained per-locus number of alleles, ranging from 5 to 14 with an average value of 9.5, perlocus observed heterozygosity, ranging from 0.07 to 0.99, and per-locus polymorphic information content (PIC), ranging from 0.34 to 0.82, indicating that some loci are highly variable. Phylogenetic analysis with the eight concatenated microsatellite loci showed no clustering on the basis of known strain characteristics. A total of 22 strain-specific apomorphic alleles, which discriminate 19 among 85 silkworm strains were obtained from eight loci. These strain-specific alleles, thus, can casually be utilized for the discrimination of applicable strains without any further typing of other loci. Furthermore, a substantial number of homozygote strains, represented by 27 among 76 alleles in eight loci were found. These results collectively suggest that the silkworm microsatellite DNA is actually and potentially important molecular markers for the eventual discrimination of silkworm strains that are preserved as hundreds in Korea.

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“…Dinucleotide microsatellites in human genetics have been reported to have higher mutation rates (1.52x10 -3 ) [34] than tetranucleotides (0.93x10 -3 ) [35], which, in addition to the high stability and ease of laboratorial manipulation of the latter [12] makes them be generally preferred for this analysis. The situation in dogs is reversed, with tetranucleotides showing higher mutation rates.…”

Section: Discussionmentioning

confidence: 99%

High Mutation Rates in Canine Tetranucleotide Microsatellites: Too Much Risk for Genetic Compatibility Purposes?

Parra¹,

García²,

Méndez³

et al. 2012

TOFORSJ

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Abstract:The use and convenience of canine tetranucleotide microsatellite markers for forensic purposes is addressed. These are commonly used in human genetics due to their high polymorphism, ease of laboratorial management and low mutation rates. However, care should be taken before including these markers in canine panels due to the high mutation rates shown in this species and the risk of errors in paternity assignment. To answer this question a panel including 16 markers (8 of them tetranucleotides) were typed for the family members of the Dogmap Project reference panel, and their mutation rates calculated. The effect these rates would have in terms of the increase in the number of false positives is assessed. Excluding one single marker with extreme mutation rate from the panel considerably improves the average rate and the performance of the panel. Therefore, a careful examination of the mutational behaviour of the candidate markers is advised for any marker panel design, even prior to the assessment of their polymorphism.

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Polymorphism characterization of five canine microsatellites

Cited by 12 publications

References 0 publications

Microsatellite analysis of the silkworm strains (Bombyx mori): high variability and potential markers for strain identification

Microsatellite analysis of the silkworm strains (Bombyx mori): high variability and potential markers for strain identification

Microsatellite Analysis of the Silkworm Strains (Bombyx mori) Originated from China

High Mutation Rates in Canine Tetranucleotide Microsatellites: Too Much Risk for Genetic Compatibility Purposes?

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