Polymorphism identification in ovine KISS1R/GPR54 gene among pure and crossbreeds of Iranian sheep

Majd, Sadreddin Anvari; Ahmadi, Ahmad; Talebi, Reza; Koohi, Pezhman Mahmoodi; Fabre, Stéphane; Qanbari, Saber

doi:10.1016/j.smallrumres.2019.02.005

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…This denotes homozygous mutant variants have a negative effect on prolific traits. According to this finding, heterozygous mutations could escalate ovulation rate and LS, whereas, in homozygous circumstances, the effects may be contradictory, from sterility to hyperprolificacy (Majd et al, 2019).…”

Section: Discussionmentioning

confidence: 95%

A Novel SNPs of KISS1 Gene Strongly Associated with Litter Size in Indonesian Goat Breeds

Febriana

Sutopo

Kurnianto

et al. 2022

Trop. Anim. Sci. J.

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Kisspeptin is a protein encoded by the KISS1 gene which behaves as a key role by stimulating gonadotropin releasing hormone (GnRH) neuron activity directly in the reproductive axis. The objective of the present study was to determine the genetic diversity within intron 1 of KISS1 gene and to verify their association with fecundity traits which can be devoted as a marker assisted selection (MAS) for breeding selection. This study was established on three Indonesian native goat populations (Kacang, Kejobong, and Senduro). The PCR products were then sequenced in both directions. The DNA sequencing alignment resulted fifteen variants (one indel and fourteen SNPs), with SNP1, SNP2, SNP3, and SNP9 being novel single nucleotide polymorphisms (SNPs) of KISS1 gene intron 1. Genotype, haplotype, and parity were significantly associated with litter size. SNP8, SNP9, and SNP10 were correlated with overall means of litter size (LS) and LS at the first and the third parities (p<0.05). Additionally, novel SNP9 were in remarkably strong linkage disequilibrium with SNP8 and SNP10 (D'=1.00; r≥0.58; χ 2 ≥13.38), and allele A had putative binding sites for the NFIC which plays an important role in activating the expression of KISS1 gene. The H2 haplotype (CATAGCGCAACGCT) was found to have the highest litter size (p<0.0001). CC genotype at SNP8, AA genotype at novel SNP9, GA genotype at SNP10, and H2 haplotype were the excellent genotypes and haplotype that associated with the superior LS (p<0.05). Therefore, this result led to presume that these three SNPs and H2 haplotype can be acknowledged as prominent genetic markers for goatbreeding selection.

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Section: Discussionmentioning

confidence: 95%

A Novel SNPs of KISS1 Gene Strongly Associated with Litter Size in Indonesian Goat Breeds

Febriana

Sutopo

Kurnianto

et al. 2022

Trop. Anim. Sci. J.

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“…Talebi, Ahmadi, Afraz, Sarry, Woloszyn et al (2018) identified several BMPR1B gene polymorphisms in the vicinity of Booroola/FecB locus and proposed that the homozygous carrier in Mehraban ewes had lamb born with lighter birth weight than heterozygous and especially homozygous non‐carrier ewes (Talebi, Ahmadi, Afraz, Sarry, Woloszyn et al, 2018). In another study on Mehraban sheep, lower birth weight among lambs of the same birth type from the genotypes of heterozygous state (C/A) compared to wild type (C/C) have been reported for the polymorphism g.3431 C>A (p.195 Phe>Leu) in exon 4 of the KISS1R / GPR54 gene (Majd et al, 2019). Insulin‐like growth factors (IGFs), e.g., IGF1 and IGF2, bind to its receptor, signaling of which mediates pathways promoting muscle hypertrophy, and inhibits biological pathways favoring muscle atrophy (Girnita et al, 2014).…”

Section: Other Important Candidate Genes With Positional and Function...mentioning

confidence: 99%

Genetic basis of muscle‐related traits in sheep: A review

et al. 2022

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Sheep is a major contributor to global food production among livestock and one of the great sources of red meat for human consumption. Several QTL and numerous genes with major and minor effects have been identified in association with the muscle characteristics in sheep breeds worldwide. Understanding the genetic background of growth and carcass-related traits in sheep is a major factor in increasing muscle growth, muscle hypertrophy and, eventually, meat production. This review concisely shows how major signaling pathways control skeletal muscle growth. Herein we aimed to discuss and summarize different research findings on genomic regions related to carcass traits and meat production in sheep. Several causative mutations with major effects on different muscle-related traits have been reported in various sheep breeds. A general overview of the studies on main candidate genes showed that some alleles have major phenotypic effects in different breeds with commercial and farm level usability. However, numerous genes with minor effects were also reported regarding the polygenic nature of muscle-related traits. The knowledge of the candidate genes involved in growth traits and their effects provides valuable information for breeding and selection of muscularity traits.

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“…The primary work is to further explore reproduction-related genes and functional loci to supply favorable conditions for exploring the genetic underpinnings of high fecundity and new variety breeding. While the main relevant major genes affecting the reproductive traits have been identified, such as BMP15 [ 16 , 17 ], GDF9 [ 17 , 18 ], B4GALNT2 [ 5 ], and KISS1R [ 19 ], their genetic roles in most sheep breeds remained unclear. In addition, the candidate genes affecting litter size varied among breeds and resulted from the combined effects of multiple candidate genes.…”

Section: Introductionmentioning

confidence: 99%

Comparative whole-genome resequencing to uncover selection signatures linked to litter size in Hu Sheep and five other breeds

Zhong,

Hou,

Zhao

et al. 2024

BMC Genomics

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Hu sheep (HS), a breed of sheep carrying the FecB mutation gene, is known for its “year-round estrus and multiple births” and is an ideal model for studying the high fecundity mechanisms of livestock. Through analyzing and comparing the genomic selection features of Hu sheep and other sheep breeds, we identified a series of candidate genes that may play a role in Hu sheep’s high fecundity mechanisms. In this study, we conducted whole-genome resequencing on six breeds and screened key mutations significantly correlated with high reproductive traits in sheep. Notably, the CC2D1B gene was selected by the fixation index (FST) and the cross-population composite likelihood ratio (XP-CLR) methods in HS and other five breeds. It was worth noting that the CC2D1B gene in HS was different from that in other sheep breeds, and seven missense mutations have been identified. Furthermore, the linkage disequilibrium (LD) analysis revealed a strong linkage disequilibrium in this specific gene region. Subsequently, by performing different grouping based on FecB genotypes in Hu sheep, genome-wide selective signal analysis screened several genes related to reproduction, such as BMPR1B and PPM1K. Besides, FST analysis identified functional genes related to reproductive traits, including RHEB, HSPA2, PPP1CC, HVCN1, and CCDC63. Additionally, a missense mutation was found in the CCDC63 gene and the haplotype was different between the high reproduction (HR) group and low reproduction (LR) group in HS. In summary, we discovered genetic differentiation among six distinct breeding sheep breeds at the whole genome level. Additionally, we identified a set of genes which were associated with reproductive performance in Hu sheep and visualized how these genes differed in different breeds. These findings laid a theoretical foundation for understanding genetic mechanisms behind high prolific traits in sheep.

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Polymorphism identification in ovine KISS1R/GPR54 gene among pure and crossbreeds of Iranian sheep

Cited by 4 publications

References 26 publications

A Novel SNPs of KISS1 Gene Strongly Associated with Litter Size in Indonesian Goat Breeds

A Novel SNPs of KISS1 Gene Strongly Associated with Litter Size in Indonesian Goat Breeds

Genetic basis of muscle‐related traits in sheep: A review

Comparative whole-genome resequencing to uncover selection signatures linked to litter size in Hu Sheep and five other breeds

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