Polymorphism in Brucella spp. due to highly repeated DNA

Halling, Shirley M.; Zehr, Emilie S.

doi:10.1128/jb.172.12.6637-6640.1990

Cited by 52 publications

(45 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Identification by standard methods is difficult and timeconsuming and may be frustrated by the occurrence of intermediates. Very minor interstrain differences were seen by polyacrylamide gel electrophoresis of proteins (12) or in some analyses with restriction endonucleases (8,14,15 fingerprinting. Polymorphic markers are also called random amplified polymorphic DNA markers (7,19,20).…”

mentioning

confidence: 99%

“…Identification by standard methods is difficult and timeconsuming and may be frustrated by the occurrence of intermediates. Very minor interstrain differences were seen by polyacrylamide gel electrophoresis of proteins (12) or in some analyses with restriction endonucleases (8,14,15). Allardet-Servent et al (1) found DNA polymorphism in Brucella strains only by using low-cleavage-frequency restriction enzymes such as XbaI or NotI.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Amplification fragment length polymorphism in Brucella strains by use of polymerase chain reaction with arbitrary primers

et al. 1992

Self Cite

View full text Add to dashboard Cite

DNA heterogeneity among members of the genus BruceUla was demonstrated with the arbitrarily primed polymerase chain reaction (AP-PCR). Simple, reproducible genomic fingerprints from DNA of 25 different BruceUla strains were generated with five arbitrarily chosen primers, alone and in pairs, with the PCR.Reaction conditions were optimized for each primer. Several DNA segments were amplified in each sample with all of the primers. PCR products that are not shared among all strains act as polymorphic markers. Polymorphism was apparent for each primer. The BruceUla strains can be distinguished according to the banding patterns of their amplified DNA on agarose gels, and the differences can be diagnostic of specific strains. To determine genetic relatedness among the Brucella strains, similarity coefficients were calculated. Statistical analysis of the similarity coefficients revealed the degrees of relatedness among strains of the genus Brucella.Six species are recognized in the genus Brucella: B. melitensis, B. abortus, B. ovis, B. suis, B. neotomae, and B. canis (5,11). These species have shown 96% + 5% relatedness to the type strain of the genus, B. melitensis ATCC 23456, in DNA hybridizations (9, 10, 17) and might have been considered pathovars of a single species were it not for their medical importance. The species differ in pathogenicity and host preference but are not typically restricted to a single host species. Four of the six recognized species are known to infect humans, though B. abortus and B. melitensis are best known as etiologic agents of human brucellosis. Identification by standard methods is difficult and timeconsuming and may be frustrated by the occurrence of intermediates. Very minor interstrain differences were seen by polyacrylamide gel electrophoresis of proteins (12) or in some analyses with restriction endonucleases (8,14,15 fingerprinting. Polymorphic markers are also called random amplified polymorphic DNA markers (7,19,20). Nucleotide polymorphisms are easier to identify with random amplified polymorphic DNA markers than with restriction fragment length polymorphism (RFLP) markers. This feature makes random amplified polymorphic markers advantageous for closely related species, like those of the genus Brucella. In the present study five different primers were used alone and in pairs to detect polymorphisms and to reveal genomic differences among 25 different Brucella strains, including strains of each of the recognized species (Table 1). MATERIALS AND METHODSSources of genomic DNA. Table 1 lists the sources of Brucella strains used in this study. The Brucella strains were supplied by Bill Deyoe from the National Animal Disease Center collection. The brucellae were cultured for 3 to 4 days on tryptose agar supplemented with heat-inactivated calf serum (5%) and agar (0.5%) under an atmosphere of 5% CO2 (2). Cultures were suspended in sterile saline (0.85% NaCl) and killed by the addition of 2 volumes of methanol.DNA was isolated from methanol-killed cells essentially as described by And...

show abstract

mentioning

confidence: 99%

“…Identification by standard methods is difficult and timeconsuming and may be frustrated by the occurrence of intermediates. Very minor interstrain differences were seen by polyacrylamide gel electrophoresis of proteins (12) or in some analyses with restriction endonucleases (8,14,15). Allardet-Servent et al (1) found DNA polymorphism in Brucella strains only by using low-cleavage-frequency restriction enzymes such as XbaI or NotI.…”

mentioning

confidence: 99%

Amplification fragment length polymorphism in Brucella strains by use of polymerase chain reaction with arbitrary primers

et al. 1992

Self Cite

View full text Add to dashboard Cite

show abstract

“…A close relationship has been found between B. melitensis and B. abortus, while an extreme divergence of B. ovis and B. neotomae from the other species was observed. This divergence may be the result of a gene conversion for this locus in these two species [48]. In this study, we found that B. neotomae strains are only partially susceptible to Tb phage, producing a few isolated plaques at RTD.…”

Section: Discussionmentioning

confidence: 59%

“…Division of the genus into species and biovars is based solely upon a small number of metabolic traits, two lipopolysaccharide epitopes, and sensitivity to a set of phage that are host-range variants of the same ancestor [48]. Although phage susceptibility to different species is evident, it indicates the significance of phage typing as a tool for Brucella species differentiation.…”

Section: Discussionmentioning

confidence: 99%

Diversity of Phage-Host Specificity in Brucella Phage

Kutateladze¹

2017

J Bacteriol Mycol

View full text Add to dashboard Cite

“…Este elemento está presente em cinco ou mais cópias no genoma de Brucella spp., mostrando ser completamente estável em número e posição no cromossomo (Halling & Zehr 1990, Bricker & Halling 1994, Bricker & Halling 1995. Entretanto, diferenças no número de elementos foram relatadas, como o biovar 1 de Brucella abortus que possui sete cópias (Ouahrani et al 1993, Bricker & Halling 1995, Ocampo-Sosa & García-Lobo 2008.…”

Section: Introductionunclassified

Avaliação genética das vacinas contra a brucelose bovina comercializadas no Brasil

et al. 2012

View full text Add to dashboard Cite

A prevenção contra infecções causadas por Brucella abortus em bovinos é realizada por meio da administração das amostras vacinais B19 e RB51. Existem relatos de que estas vacinas podem causar aborto em fêmeas vacinadas. Portanto, toda a ocorrência de aborto em animais vacinados merece um estudo aprofundado sobre a causa. No Brasil, não há registro sobre a origem das amostras B19 e RB51 utilizadas na produção das vacinas comerciais. Assim, um estudo para verificar possíveis mutações em relação às amostras referência USDA B19 e USDA RB51 de B. abortus se faz necessário, devido às amostras vacinais poderem reverter a sua virulência. Objetivou-se com este estudo caracterizar genotipicamente as amostras vacinais B19 e RB51 comercializadas no Brasil. A metodologia utilizada foi a genotipagem de genes marcadores destas amostras vacinais, por meio da amplificação pela reação em cadeia da polimerase. Os resultados obtidos permitiram a identificação do genótipo das vacinas comerciais B19 e RB51 disponíveis e utilizadas em bovinos no Brasil. A ausência de mutações nas vacinas testadas corrobora com a qualidade genética das mesmas, quanto à estabilidade dos genes analisados.

show abstract

Polymorphism in Brucella spp. due to highly repeated DNA

Cited by 52 publications

References 23 publications

Amplification fragment length polymorphism in Brucella strains by use of polymerase chain reaction with arbitrary primers

Amplification fragment length polymorphism in Brucella strains by use of polymerase chain reaction with arbitrary primers

Diversity of Phage-Host Specificity in Brucella Phage

Avaliação genética das vacinas contra a brucelose bovina comercializadas no Brasil

Contact Info

Product

Resources

About