Polymorphism in exon 2 of the BuLA-DRB3 gene in Indian buffalo (<i>Bubalus bubalis</i>var.<i>indicus</i>) detected by PCR-RFLP

Aravindakshan, T. V.; Nainar, A. Mahalinga; Sivaselvam, S.N.

doi:10.1017/s1357729800054680

Cited by 10 publications

(15 citation statements)

References 14 publications

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“…Aravindakshan et al (2000) and Kumar et al (2011) found the same four patterns in Murrah buffaloes, but additionally identified a pattern not found here; this pattern was characterized by the absence of an HaeIII restriction site (304 bp). Kumar et al (2011) reported that HaeIII-a was most common in Murrah buffaloes (0.46), while Aravindakshan et al (2000) found the HaeIII-b pattern to be most frequent in this breed (0.38). In Surti buffaloes, the HaeIII-a pattern was the most frequent (0.43) (Aravindakshan et al, 2000).…”

Section: C Rsai-c/n Rsai-f/s Rsai-h/l Rsai-h/m Rsai-h/o Rsai-l/supporting

confidence: 77%

“…Murrah buffaloes had four RFLP patterns (RsaI-l, RsaI-n, RsaI-o, and RsaI-s) and six different genotypes. Aravindakshan et al (2000) found that RsaI-o and RsaI-s patterns were the most frequent in Murrah buffaloes with a frequency of 0.25 for each patterns, while RsaI-l was the most frequent in Surti buffaloes (0.24). Kumar et al (2011) reported RsaI-i (183/67/54 bp) and RsaI-f digestion patterns as the most common in Murrah with a frequency of 0.22.…”

Section: C Rsai-c/n Rsai-f/s Rsai-h/l Rsai-h/m Rsai-h/o Rsai-l/mentioning

confidence: 90%

“…Kumar et al (2011) reported that HaeIII-a was most common in Murrah buffaloes (0.46), while Aravindakshan et al (2000) found the HaeIII-b pattern to be most frequent in this breed (0.38). In Surti buffaloes, the HaeIII-a pattern was the most frequent (0.43) (Aravindakshan et al, 2000). Kumar et al (2008) found that the HaeIII-b pattern was the most frequent in Nili-Ravi buffaloes (0.60) while Acharya et al (2002) reported HaeIII-a as the most frequent in Jafarabadi (0.42) and HaeIII-b as the most frequent in Mehsani buffaloes (0.64).…”

Section: C Rsai-c/n Rsai-f/s Rsai-h/l Rsai-h/m Rsai-h/o Rsai-l/mentioning

confidence: 97%

“…The agarose gels were photographed under UV light with a Kodak digital camera DC290 and analyzed with the program Kodak Electrophoresis Documentation and Analysis System (EDAS). Allele frequencies and number of alleles were obtained by direct counting and named according to van Eijk et al (1992) and Aravindakshan et al (2000).…”

Section: Restriction Fragment Length Polymorphism Analysismentioning

confidence: 99%

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Analysis of DRB3 gene polymorphisms in Jafarabadi, Mediterranean, and Murrah buffaloes from Brazil

Stafuzza¹,

Olivatto²,

Naressi³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The DRB3 gene is an MHC class II gene that has a high degree of polymorphism with more than 100 alleles described in cattle. This variation contributes to differences among individuals in immune responsiveness and disease resistance. In this study, we searched for allelic variants in exon 2 of the DRB3 gene in 80 river buffaloes of three breeds in Brazil using a PCR-RFLP technique. The PCR product showed genetic polymorphism when digested with RsaI, PstI or HaeIII restriction enzymes. In total, 16 restriction patterns were identified: nine restriction patterns and 16 genotypes were found with RsaI; four restriction patterns and nine genotypes were found with HaeIII; and, three restriction patterns and four genotypes were found with PstI. Three RFLP patterns were exclusive to Jafarabadi buffaloes (RsaI-b, RsaI-c and RsaI-f) and three others were only observed in Mediterranean buffaloes (RsaI-g, RsaI-h and PstI-y). Jafarabadi buffaloes had a larger number of RFLP patterns than Mediterranean and Murrah breeds. The analysis showed that the DRB3 exon 2 was highly polymorphic, with the highest degree of polymorphism in Mediterranean buffaloes. This study provides the first assessment of allelic variation among three different buffalo breeds from Brazil and provides a basis for further investigations into the association between the DRB3 alleles and disease resistance.

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Section: C Rsai-c/n Rsai-f/s Rsai-h/l Rsai-h/m Rsai-h/o Rsai-l/supporting

confidence: 77%

Section: C Rsai-c/n Rsai-f/s Rsai-h/l Rsai-h/m Rsai-h/o Rsai-l/mentioning

confidence: 90%

Section: C Rsai-c/n Rsai-f/s Rsai-h/l Rsai-h/m Rsai-h/o Rsai-l/mentioning

confidence: 97%

Section: Restriction Fragment Length Polymorphism Analysismentioning

confidence: 99%

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Analysis of DRB3 gene polymorphisms in Jafarabadi, Mediterranean, and Murrah buffaloes from Brazil

Stafuzza¹,

Olivatto²,

Naressi³

et al. 2016

Genet. Mol. Res.

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“…Analysis indicated that the class II genes fall into two linkage groups consistent with the organization of the MHC in cattle. Aravindakshan et al 2000 [59] referred to the MHC gene in buffaloes as BuLA though it is more often referred to as the Bubu-DRB3 gene [60]. …”

Section: The Major Histocompatibility Complex Class Iia Subregionmentioning

confidence: 99%

The Major Histocompatibility Complex in Bovines: A Review

Behl

Verma

Tyagi

et al. 2012

ISRN Veterinary Science

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Productivity in dairy cattle and buffaloes depends on the genetic factors governing the production of milk and milk constituents as well as genetic factors controlling disease resistance or susceptibility. The immune system is the adaptive defense system that has evolved in vertebrates to protect them from invading pathogens and also carcinomas. It is remarkable in the sense that it is able to generate an enormous variety of cells and biomolecules which interact with each other in numerous ways to form a complex network that helps to recognize, counteract, and eliminate the apparently limitless number of foreign invading pathogens/molecules. The major histocompatibility complex which is found to occur in all mammalian species plays a central role in the development of the immune system. It is an important candidate gene involved in susceptibility/resistance to various diseases. It is associated with intercellular recognition and with self/nonself discrimination. It plays major role in determining whether transplanted tissue will be accepted as self or rejected as foreign.

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Five BoLA-DRB3 genotypes detected in Egyptian buffalo infected with Foot and Mouth disease virus serotype O

Othman

Khodary

El‐Deeb

et al. 2018

Journal of Genetic Engineering and Biotechnology

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Foot and Mouth disease (FMD) is a contagious disease leads to economically loss in livestock production all over the world. This serious disease is caused due to the infection of the animal with a single-stranded RNA virus (FMDV). This study aimed to investigate the genetic polymorphism of BoLA-DRB3 gene in Egyptian buffalo as a candidate genetic marker included in multi-factorial process of FMD resistance/susceptibility. Also this work aimed to genetically characterization and serotyping of circulating FMD virus in Egypt during 2016.For serotyping of FMDV, RT-PCR was used for FMDV-positive samples and the results declared the presence of serotype O in all tested animals. The sequence analysis of FMDV samples revealed five different patterns for the detected serotype O which were submitted to GenBank under the accession Nos.: MG017361–MG017365.The 302-bp amplified fragments from BoLA-DRB3 exon 2 were digested with HaeIII endonuclease and the results showed that the presence of five BoLA-DRB3 genotypes, among them the genotype AA might be associated with FMD-resistance (P < 0.01). On the other hand, genotype AC could be correlated with susceptibility (P < 0.01) to FMD in Egyptian buffaloes where it was absent in resistant group. The five detected genotypes of BoLA-DRB3 exon 2 were submitted to GenBank with the accession Nos.: MF977316–MF977320. In conclusion, our findings suggested that the detection of different BoLA-DRB3 genotypes may be has a promising role for raising the resistance of Egyptian buffalo against FMDV especially serotype O which is prevalent in Egypt with preferring genotype AA.

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Polymorphism in exon 2 of the BuLA-DRB3 gene in Indian buffalo (Bubalus bubalisvar.indicus) detected by PCR-RFLP

Cited by 10 publications

References 14 publications

Analysis of DRB3 gene polymorphisms in Jafarabadi, Mediterranean, and Murrah buffaloes from Brazil

Analysis of DRB3 gene polymorphisms in Jafarabadi, Mediterranean, and Murrah buffaloes from Brazil

The Major Histocompatibility Complex in Bovines: A Review

Five BoLA-DRB3 genotypes detected in Egyptian buffalo infected with Foot and Mouth disease virus serotype O

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