Polymorphism in Myristoylpalmitoylphosphatidylcholine

Tristram-Nagle, Stephanie; Isaacson, Yisrael; Lyatskaya, Yulia; Liu, Yufeng; Brummond, Kay M.; Katsaras, John; Nagle, John F.

doi:10.1016/s0009-3084(99)00041-9

Cited by 15 publications

(12 citation statements)

References 45 publications

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“…1 h can induce the L c phase. 27 The present results suggest that pressure further accelerates the transformation into the L c phase in the MPPC bilayer. For confirmation of the L c -phase spectra of Prodan in the MPPC bilayer, we investigated the time dependence of the L c phase-spectra at 15.3°C and 224.9 MPa.…”

Section: Resultssupporting

confidence: 52%

Study on the Subgel-Phase Formation Using an Asymmetric Phospholipid Bilayer Membrane by High-Pressure Fluorometry

et al. 2012

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The myristoylpalmitoylphosphatidylcholine (MPPC) bilayer membrane shows a complicated temperature-pressure phase diagram. The large portion of the lamellar gel (L(β)'), ripple gel (P(β)'), and pressure-induced gel (L(β)I) phases exist as metastable phases due to the extremely stable subgel (L(c)) phase. The stable L(c) phase enables us to examine the properties of the L(c) phase. The phases of the MPPC bilayers under atmospheric and high pressures were studied by small-angle neutron scattering (SANS) and fluorescence spectroscopy using a polarity-sensitive fluorescent probe Prodan. The SANS measurements clearly demonstrated the existence of the metastable L(β)I phase with the smallest lamellar repeat distance. From a second-derivative analysis of the fluorescence data, the line shape for the L(c) phase under high pressure was characterized by a broad peak with a minimum of ca. 460 nm. The line shapes and the minimum intensity wavelength (λ″(min)) values changed with pressure, indicating that the L(c) phase has highly pressure-sensible structure. The λ″(min) values of the L(c) phase spectra were split into ca. 430 and 500 nm in the L(β)I phase region, which corresponds to the formation of a interdigitated subgel L(c) (L(c)I) phase. Moreover, the phase transitions related to the L(c) phase were reversible transitions under high pressure. Taking into account the fluorescence behavior of Prodan for the L(c) phase, we concluded that the structure of the L(c) phase is highly probably a staggered structure, which can transform into the L(c)I phase easily.

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Section: Resultssupporting

confidence: 52%

Study on the Subgel-Phase Formation Using an Asymmetric Phospholipid Bilayer Membrane by High-Pressure Fluorometry

et al. 2012

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“…It may also be recalled that the lipid MPPC doesn't even have a gel phase; 32 this is consistent with MPPC having two fewer methylenes on its sn-1 chain than on its sn-2 chain, and therefore likely to have less mini-interdigitation. Another notable modeling result is that the greater range of |F(q)| data gives higher resolution in the electron density profile in Fig.…”

Section: Discussionmentioning

confidence: 66%

Structure of gel phase DPPC determined by X-ray diffraction

Nagle

Cognet

Dupuy

et al. 2019

Chemistry and Physics of Lipids

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High resolution low angle x-ray data are reported for the gel phase of DPPC lipid bilayers, extending the previous q range of 1.0 Å -1 to 1.3 Å -1 , and employing a new technique to obtain more accurate intensities and form factors |F(q)| for the highest orders of diffraction. Combined with previous wide angle x-ray and volumetric data, a space filling model is employed to obtain gel phase structure at a mesoscopic level. A new conclusion from this analysis is that the hydrocarbon chains from opposing monolayers are mini-interdigitated; this would help explain the previously well-established result that the opposing monolayers are strongly coupled with respect to their chain tilt directions.Even more detailed structural features are described that have not been obtained from experiment but that could, in principle, be obtained from simulations that would first be validated by agreement with the wide angle and the new low angle |F(q)| x-ray data.

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“…They also share with MPPC the property of having no gel phase (Serrallach et al, 1984), although MPPC does have a ripple phase, which these lipids do not have. Mini-interdigitation was proposed to be an important factor for MPPC subgel formation (Tristram-Nagle et al, 1999); the chains of the (Cn) 2 -Glu-C 2 -COOH lipids appear to be fully interdigitated in the subgel phase so this predisposition is already present. As the chain length increases, the total van der Waals interactions between chains increases.…”

Section: Discussionmentioning

confidence: 99%

Thermodynamic and structural characterization of amino acid-linked dialkyl lipids

Tristram-Nagle

Lewis

Blickenstaff

et al. 2005

Chemistry and Physics of Lipids

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Using differential scanning calorimetry (DSC), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), we determined some thermodynamic and structural parameters for a series of amino acid-linked dialkyl lipids containing a glutamic acid-succinate headgroup and di-alkyl chains: C12, C14, C16 and C18 in CHES buffer, pH 10. Upon heating, DSC shows that the C12, C14 and annealed C16 lipids undergo a single transition which XRD shows is from a lamellar, chain ordered subgel phase to a fluid phase. This single transition splits into two transitions for C18, and FTIR shows that the upper main transition is predominantly the melting of the hydrocarbon chains whereas the lower transition involves changes in the headgroup ordering as well as changes in the lateral packing of the chains. For short incubation times at low temperature, the C16 lipid appears to behave like the C18 lipid, but appropriate annealing at low temperatures indicates that its true equilibrium behavior is like the shorter chain lipids. XRD shows that the C12 lipid readily converts into a highly ordered subgel phase upon cooling and suggests a model with untilted, interdigitated chains and an area of 77.2A(2)/4 chains, with a distorted orthorhombic unit subcell, a=9.0A, b=4.3A and beta=92.7 degrees . As the chain length n increases, subgel formation is slowed, but untilted, interdigitated chains prevail.

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Polymorphism in Myristoylpalmitoylphosphatidylcholine

Cited by 15 publications

References 45 publications

Study on the Subgel-Phase Formation Using an Asymmetric Phospholipid Bilayer Membrane by High-Pressure Fluorometry

Study on the Subgel-Phase Formation Using an Asymmetric Phospholipid Bilayer Membrane by High-Pressure Fluorometry

Structure of gel phase DPPC determined by X-ray diffraction

Thermodynamic and structural characterization of amino acid-linked dialkyl lipids

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