Polymorphism of exon 2 of BoLA-DRB3 gene and its relationship with somatic cell score in Beijing Holstein cows

Abstract

In the present study, the exon 2 and 3' end sequence of intron 1 of BoLA-DRB3 gene of 211 Beijing Holstein cows was amplified and a uniform fragment of 284 bp was obtained. The genetic polymorphism was investigated by PCR-RFLP using two restriction endonucleases HaeIII and BstYI. Seven genotypes were detected by digesting the PCR products with HaeIII. The frequency of AA, AB, AC, AD, BB, BC and BF genotypes was 0.4638, 0.0193, 0.0193, 0.3478, 0.0290, 0.0386 and 0.0822, respectively. Three genotypes were found by digesting the PCR products with BstYI. The frequency of AA, AB and BB genotypes was 0.0569, 0.2844 and 0.6587, respectively. The relationship between the polymorphisms in exon 2 of BoLA-DRB3 gene and somatic cell score (SCS) in Beijing Holstein cows was analyzed by least squares linear model. No significant difference was detected among least squares means of SCS for seven HaeIII-RFLP genotypes (P > 0.05). As for BstYI-RFLP analysis, least squares mean of SCS for AA was significantly lower than that for AB (P < 0.05) or BB (P < 0.05), and no significant difference was detected between AB and BB genotypes (P > 0.05). BstYI AA was the most favorable genotype and BstYI BB was the most unfavorable genotype for mastitis resistance. The information found in the present study is very important for improving mastitis resistance in dairy cattle by marker assisted selection.

Bovine Mastitis: Frontiers in Immunogenetics

“…The major histocompatability complex (MHC) plays an essential role in the induction and regulation of immune responses ( 39 ). The bovine MHC, bovine lymphocyte antigen (BoLA), has been associated with resistance or susceptibility to mastitis ( 40 – 43 ), somatic cell count ( 42 , 44 , 45 ), and immune response ( 40 , 41 , 46 ). Genetic variation, such as single nucleotide polymorphisms (SNP) in other candidate genes associated with resistance or susceptibility to mastitis have been identified, including TLR4 ( 47 , 48 ), TLR2, and caspase-recruitment domain 15 ( 49 ); IL-10 ( 50 ), osteopontin ( 51 ), IL-8 and its receptor CXCR1 ( 52 – 54 ), CCL2 and its receptor ( 55 ), as well as a variety of other genes ( 56 ).…”

Detection of quantitative trait loci and putative causal variants affecting somatic cell score in dairy sheep by using a 50K SNP chip and whole-genome sequencing

“…Among the 23 harboring genes of these mutations, obvious candidates are included in the ovine MHC (HLA-DRB1, HLA-DQA1, DQA, HLa-B, HLA-C, and BoLA-HLA-A). In cattle, bovine lymphocyte antigen (BoLA) has been associated with resistance to mastitis (Mallard et al, 1995;Rupp et al, 2007) and SCC (Sharif et al, 1998;Chu et al, 2012), with several studies supporting the direct association of these traits with the DRB3 locus. In addition, in Holstein cattle, heterozygosity of the BoLA-DQA1 gene has been associated with resistance to mastitis progression (Takeshima et al, 2008).…”

Melhoramento genético visando à resistência a mastite em bovinos leiteiros

“…O gene BoLA (Bovine Lymphocyte Antigen) tem sido amplamente estudado nos últimos 20 anos em razão de sua influência sobre as características produtivas e às relacionadas à saúde animal, localiza-se no Complexo Principal de Histocompatibilidade (MHCMajor Histocompatibility Complex) do genoma bovino e que são altamente polimórficos, estão envolvidos nos processos celulares de resistência ou suscetibilidade à mastite (Rupp et al, 2007), CCS (Pashmi et al, 2009;Chu et al, 2012), e resposta imunitária (Thompson-Crispi et al, 2014a).…”