POLYMORPHISM OF GRAIN SORGHUM FROM VIR WORLD COLLECTION FOR THE CHARACTERS ASSOCIATED WITH THE CMS-Rf GENETIC SYSTEM

Anisimova, I. N.; Ryabova, Daria; Malinovskaya, E. V.; Alpatieva, N. V.; Karabitsina, Yu. I.; Radchenko, E. E.

doi:10.15389/agrobiology.2017.5.952eng

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…The R-lines grown under controlled greenhouse conditions produced highly fertile pollen (fertility rate 97-100%). The fertility pollen indices of the earlier inbred generations of the same lines grown in the field at KES VIR, turned out to be somewhat lower and varied from 57 to 83%, which can be explained by the effects of environmental conditions (temperature, moisture availability) [27].…”

Section: Discussionmentioning

confidence: 95%

“…The numbering of the sequence is according to alignment with a reference sequence Sobic.002G057050, presented in the PLAZA nucleotide sequence database. With the use of STS primers 2459403fw and 2459403rev [27], a 935 bp fragment of the Rf2 gene localized in the range of 337-1271 bp of the reference sequence was amplified (Table S3). PCR was carried out in a reaction mixture of 25 µL containing 50 ng of genomic DNA, 2.5 µL of 10× buffer, 1.5 µL of 50× MgCl 2 , and 0.5 µL of 10 rM primers: 2.4 µL of a mixture of nucleotides with a concentration of 2.5 mM each and 1 U Taq DNA polymerase.…”

Section: Caps Marker Development and Rf2 Alleles Confirmationmentioning

confidence: 99%

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Newly Developed Restorer Lines of Sorghum [Sorghum bicolor (L.) Moench] Resistant to Greenbug

Radchenko,

Anisimova,

Ryazanova

et al. 2024

Plants

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Eight lines of grain sorghum [Sorghum bicolor (L.) Moench], which can be used as a promising source material in heterotic hybrid breeding as pollen fertility restorers and donors of resistance to the greenbug (Schizaphis graminum Rondani), are characterized. The new restorer lines (R-lines) were developed by crossing the maternal sterile line Nizkorosloe 81s (CMS A1) with two lines selected from the grain sorghum collection accessions VIR-928 and VIR-929 as the paternal forms. The R-lines were genotyped using PCR markers, and also characterized by height, duration of the seedling–flowering period, and some of the technological properties of flour. With the use of microsatellite markers linked to the Rf genes and by hybridological analysis, it was shown that the new lines carry the dominant allele of the gene Rf2. The PCoA analysis demonstrated clear differences of each R-line from the parents. The genotypes of the new lines and their parental forms for the Rf2 locus were confirmed by applying three allele-specific codominant CAPS markers which detected SNPs in the candidate Rf2 gene. All new lines were highly fertile, as demonstrated by cytological analysis of acetocarmine-stained pollen preparations. A high resistance to the greenbug was demonstrated for each new R-line both in the laboratory and field conditions against a severe aphid infestation. Grain quality parameters such as protein content and dough rheological properties varied widely and were quite satisfactory in some R-lines. Characteristics common to all eight sorghum lines studied, such as the ability to restore pollen fertility in the F1 generation, good pollen quality, greenbug resistance, early ripening, spreading panicle, and low stature, allow us to recommend them for producing commercial F1 hybrids with satisfactory grain quality for both fodder and food purposes.

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Section: Discussionmentioning

confidence: 95%

Section: Caps Marker Development and Rf2 Alleles Confirmationmentioning

confidence: 99%

Newly Developed Restorer Lines of Sorghum [Sorghum bicolor (L.) Moench] Resistant to Greenbug

Radchenko,

Anisimova,

Ryazanova

et al. 2024

Plants

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Development and validation of CAPS-marker associated with the <i>Rf2</i> gene in sorghum (<i>Sorghum bicolor</i> (L.) Moench)

Radchenko

Alpatieva

Karabitsina

et al. 2021

Biotehnologiâ i selekciâ rastenij

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Background. The development of heterotic hybrids based on cytoplasmic male sterility (CMS) is the leading strategy in breeding sorghum (Sorghum bicolor (L.) Moench). The trait of pollen fertility restoration in forms with CMS A1 (milo), predominantly used in sorghum breeding, is determined by at least two dominant complementary genes Rf1 and Rf2, and also gene Rf5. The development of accessible molecular markers of sorghum Rf genes is highly relevant for hybrid breeding, since they can significantly accelerate the process of creating female sterile forms (A lines), sterility maintainers (B lines) and pollen fertility restorers (R lines).Material and methods. The studied material included 36 sorghum accessions from the VIR collection, which differed by the ability to restore pollen fertility in forms with A1-type CMS. The nucleotide polymorphism of 935 bp fragments of the PPR genes Sobic.002G057050, Sobic.002G054100, and Sobic.002G054200 located at the chromosome 2 was studied.Results. The fragments obtained with the use of a pair of 2459403fw and 2459403 primers were 935 bp long and included parts of three genes: Sobic.002G057050, Sobic.002G054100, Sobic.002G054200. For identifying the sequence variant Sobic.002G057050-1090 associated with the Rf2 gene, Tru9 I restrictase was chosen, which allows obtaining a 572 bp fragment unique for all the studied R lines. Such a marker was found in 10 sorghum lines from West China and Kyrgyzstan, which are widely used in breeding as fertility restorers. The fragment was found neither in three lines with sterile cytoplasm and their fertile analogues, nor in 7 accessions of kafir sorghum, which lacked functional alleles of Rf genes.Conclusions. It has been demonstrated that the marker can be used for selection and checking purity of R and B/A lines. It is also applicable for verifying hybridity of F1 seeds and analyzing hybrid populations from crosses of R lines 924-4, 928-1, 929-3, 931-1, 933-1/6, 1237-3, 1243-2, 1251, 1150-1, F10BC2 with A lines Nizkorosloe 81s, А-83 and А-10598. It may be suggested that the ability to restore pollen fertility in R lines, which lack the marker CAPS- 572, is determined by another Rf gene. The studied 935 bp fragment of Sobic.002G057050 harbours 22 SNP, therefore the development of CAPS-markers for their identification and differentiation can be promising.

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POLYMORPHISM OF GRAIN SORGHUM FROM VIR WORLD COLLECTION FOR THE CHARACTERS ASSOCIATED WITH THE CMS-Rf GENETIC SYSTEM

Cited by 2 publications

References 26 publications

Newly Developed Restorer Lines of Sorghum [Sorghum bicolor (L.) Moench] Resistant to Greenbug

Newly Developed Restorer Lines of Sorghum [Sorghum bicolor (L.) Moench] Resistant to Greenbug

Development and validation of CAPS-marker associated with the <i>Rf2</i> gene in sorghum (<i>Sorghum bicolor</i> (L.) Moench)

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