Polymorphism of the CAST gene and association of its genotypes with indicators of sheep meat productivity

Abstract

The article presents studies aimed at a comparative assessment of the meat productivity and quality of meat of young sheep of the Stavropol breed of different genotypes (CASTNN and CASTMM) identified by PCR – PDRF and studies of chemical and histological parameters. According to the results of the control slaughter, the superiority of the CASTNN genotype over the CASTMM genotype analogue in terms of the pre-slaughter mass by 9.1%, the mass of the paired carcass and its yield by 12.3 and 3.4% was revealed. Indicators of the morphological composition of CASTNN genotype carcasses meet the requirements of GOST I category. Morphometric analysis indicates a smaller number of muscle, connective-woven fibers, but a larger diameter of muscle fibers in the meat of the CASTNN genotype than in the CASTMM meat, which provided it with a higher score of "marbling": 32.41 vs. 27.17. To identify particularly valuable animals and their wide application in the breeding process, it is advisable to use the PCR analysis method in conjunction with morpho-histological studies.

The Use of Genotyping to Select Desired-Type Animals

“…На сегодняшний день появилась возможность выявления значительного числа генетических полиморфизмов на основе последовательности ДНК и использования их как маркеров с целью оценки генетической основы наблюдаемой фенотипической изменчивости признаков [5,11,13].…”