Polymorphism of the Myostatin (MSTN) Gene in Landes and Kielecka Geese Breeds

Abstract

Simple Summary: Identification of mutations in the myostatin gene, affecting the occurrence of the double muscling phenotype in some breeds of beef cattle, was an impetus for further analysis and identification of mutations within this gene in other animal breeds, characterized by increased meat performance parameters. The number of geese in poultry livestock production in Poland is small. The native geese breeds can be successfully used to produce high-quality poultry meat and can be a very good source of goose meat production for regional and organic products. The aim of the study was to identify a mutation in the MSTN (Myostatin) gene and investigate whether this polymorphism can affect body weight in different periods of life in Landes and Kielecka breeds. Measurements of the examined trait were taken with time intervals to demonstrate the putative effect of the identified SNP (Single Nucleotide Polymorphism) on body weight over the course of bird growth. In conclusion, the identified c.1231C>T polymorphism suggests a possible link between the polymorphism and the BW (body weight) of Kielecka geese in the 12th week of life. The most significant factors affecting the BW values in geese are breed and sex.Abstract: Myostatin, also known as growth differentiation factor 8 (GDF8), belongs to the TGF-β superfamily of proteins. MSTN is a highly conserved protein that acts as a negative regulator of skeletal muscle growth. Loss of myostatin functionality causes the phenotype to appear in the form of 'double musculature', among others in cattle, sheep, and house mice. The presented results of the research were carried out on two geese breeds-Landes and Kielecka. The aim of the study was to identify mutations in the MSTN gene and study their impact on body weight in both geese breeds in different periods of life. Analysis of the obtained results showed the existence of polymorphism in exon 3 (c.1231C>T) and suggested a possible association (p < 0.05) between BW and genotype in 12 weeks of life in male Kielecka geese breed. The identified polymorphism may be one of the factors important for improving body weight in the studied Kielecka breed, therefore, it is necessary to conduct further research on a larger population of geese breeds in order to more accurately estimate the effect of the identified SNP c.1231C>T on BW in geese.

Identification and Validation of Marketing Weight-Related SNP Markers Using SLAF Sequencing in Male Yangzhou Geese

“…Using reduced representation (RR) sequencing, 277,362 SNPs were detected for pink-footed goose [48]. In additional, one SNP in exon three of Myostatin gene was detected in Landes and Kielecka geese breeds [49]. Recently, a total of 26 SNPs and 14 annotated genes significantly associated with quality traits and egg production were identified in Sichuan white geese by GWAS [50].…”

A novel (ATC)n microsatellite locus is associated with litter size in an indigenous Chinese pig

“…The specific primers and genome DNA of Qinghai Bamei sows were used to perform polymerase chain reaction (PCR; Smołucha et al, 2019 ). The products were sent to Saisike Biotechnology Co., Ltd. for TOF-MS analyses.…”

A Preliminary Investigation of Myostatin Gene (MSTN) Variation in Red Deer (Cervus elaphus) and Its Implications for Venison Production in New Zealand

“…Accordingly, research on MSTN variation and its effect on meat production has been of great interest to livestock farming. Variation in MSTN has been associated with various meat production traits in cattle [2][3][4][5], sheep [6,7], goats [8][9][10], pigs [11,12], rabbits [13,14], chicken [15,16], and geese [17].…”